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小鼠热休克蛋白60(Hsp-60)ELISA试剂盒*新闻快讯
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小鼠热休克蛋白60(Hsp-60)elisa试剂盒*新闻快讯操作注意事项
1. 试剂应按标签说明书储存,使用前恢复到室温。稀稀过后的标准品应丢弃,不可保存。
2. 实验中不用的板条应立即放回包装袋中,密封保存,以免变质。
3. 不用的其它试剂应包装好或盖好。不同批号的试剂不要混用。保质前使用。
4. 使用一次性的吸头以免交叉污染,吸取终止液和底物A、B液时,避免使用带金属部分的加样器。
5. 使用干净的塑料容器配置洗涤液。使用前充分混匀试剂盒里的各种成份及样品。
6. 洗涤酶标板时应充分拍干,不要将吸水纸直接放入酶标反应孔中吸水。
7. 底物A应挥发,避免长时间打开盖子。底物B对光敏感,避免长时间暴露于光下。避免用手接触,有毒。实验完成后应立即读取OD值。
8. 加入试剂的顺序应一致,以保证所有反应板孔温育的时间一样。
9. 按照说明书中标明的时间、加液的量及顺序进行温育操作。
小鼠热休克蛋白60(Hsp-60)ELISA试剂盒*新闻快讯样品收集、处理及保存方法
1、 血清-----操作过程中避免任何细胞刺激。使用不含热原和内毒素的试管。收集血液后,1000×g离心10分钟将血清和红细胞迅速小心地分离。
2、 血浆-----EDTA、柠檬酸盐、肝素血浆可用于检测。1000×g离心30分钟去除颗粒。
3、 细胞上清液---1000×g离心10分钟去除颗粒和聚合物。
4、 组织匀浆-----将组织加入适量生理盐水捣碎。1000×g离心10分钟,取上清液
5、 保存------如果样品不立即使用,应将其分成小部分-70 ℃保存,避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒,检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。
小鼠热休克蛋白60(Hsp-60)ELISA试剂盒*新闻快讯相关产品发布:
EIF2AK1 Antibody | Q9BQI3 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 94%, Positives = 94%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
EIF2S2 Antibody | P20042 | Human (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:5000 | |
EIF1AY Antibody | O14602 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 92%, Positives = 100%);Rat (Identities = 92%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:5000 | |
ERD23 Antibody | O43731 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:20000 | |
ERD22 Antibody | P33947 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000 | |
EPN3 Antibody | Q9H201 | Human (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 | |
EPN2 Antibody | O95208 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:20000 | |
EPHB1/2/3 Antibody | P54762/P29323/P54753 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 | |
EPHA10 Antibody | Q5JZY3 | Human (Identities = 100%, Positives = 100%)Mouse (Identities = 92%, Positives = 92%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
MAN1B1 Antibody | Q9UKM7 | Human | WB: 1:500~1:1000 ELISA: 1:1000 | |
NEIL3 Antibody | Q8TAT5 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
NEIL1 Antibody | Q96FI4 | Human (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:20000 | |
ENDOGL1 Antibody | Q9Y2C4 | Human | WB: 1:500~1:1000 ELISA: 1:10000 | |
ELP2 Antibody | Q6IA86 | Human (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:10000 | |
ELOVL6 Antibody | Q9H5J4 | Human (Identities = 100%, Positives = 100%)Mouse (Identities = 100%, Positives = 100%) Rat (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:10000 | |
ELOVL5 Antibody | Q9NYP7 | Human (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000 | |
ELOVL4 Antibody | Q9GZR5 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
ELOVL3 Antibody | Q9HB03 | Human (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:20000 | |
ELOVL2 Antibody | Q9NXB9 | Human (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:10000 | |
ELOVL1 Antibody | Q9BW60 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:40000 | |
TUFM Antibody | P49411 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:40000 | |
GFM2 Antibody | Q969S9 | Human | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000 | |
EEF1G Antibody | P26641 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 92%, Positives = 100%);Rat (Identities = 92%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:20000 | |
EFEMP2 Antibody | O95967 | Human | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 | |
EFEMP1 Antibody | Q12805 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:10000 | |
ENTPD1 Antibody | P49961 | Human (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:20000 | |
INSL4 Antibody | Q14641 | Human | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000 | |
CD69 Antibody | Q07108 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 92%, Positives = 92%);Rat (Identities = 92%, Positives = 92%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
ZNRF2 Antibody | Q8NHG8 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 91%, Positives = 91%) | IF: 1:100~1:500 ELISA: 1:20000 | |
UBR1 Antibody | Q8IWV7 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:20000 | |
RNF19A Antibody | Q9NV58 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 92%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
RNF149 Antibody | Q8NC42 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 92%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:10000 | |
RNF144B Antibody | Q7Z419 | Human; Mouse | WB: 1:500~1:1000 ELISA: 1:40000 | |
RNF144A Antibody | P50876 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:40000 | |
RNF14 Antibody | Q9UBS8 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 92%, Positives = 100%); Rat (Identities = 92%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
RNF138 Antibody | Q8WVD3 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 92%, Positives = 100%); Rat (Identities = 92%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:20000 | |
RNF125 Antibody | Q96EQ8 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:10000 | |
RFWD2 Antibody | Q8NHY2 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:20000 | |
RNF111 Antibody | Q6ZNA4 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
PJA2 Antibody | O43164 | Human (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
MYLIP Antibody | Q8WY64 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 | |
MARCH5 Antibody | Q9NX47 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IF: 1:100~1:500 ELISA: 1:40000 | |
MARCH4 Antibody | Q9P2E8 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 92%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:40000 | |
MARCH3 Antibody | Q86UD3 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000 | |
MARCH2 Antibody | Q9P0N8 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 78%, Positives = 78%);Rat (Identities = 78%, Positives = 78%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000 | |
HECW2 Antibody | Q9P2P5 | Human (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:5000 | |
DZIP3 Antibody | Q86Y13 | Human (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000 | |
CCNB1IP1 Antibody | Q9NPC3 | Human | WB: 1:500~1:1000 ELISA: 1:40000 | |
RNF111 Antibody | Q6ZNA4 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
DYNLRB2 Antibody | Q8TF09 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 91%, Positives = 91%) | IHC: 1:50~1:100 ELISA: 1:10000 | |
DNAL4 Antibody | O96015 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 92%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:40000 | |
DYNLL2 Antibody | Q96FJ2 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:5000 | |
DNAL1 Antibody | Q4LDG9 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:40000 | |
DNAI2 Antibody | Q9GZS0 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:10000 | |
DNAI1 Antibody | Q9UI46 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 92%, Positives = 100%); Rat (Identities = 84%, Positives = 92%) | WB: 1:500~1:1000 ELISA: 1:20000 | |
TESK1 Antibody | Q15569 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 92%, Positives = 92%); Rat (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
DBH Antibody | P09172 | Human (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:5000 | |
DNAJC5 Antibody | Q9H3Z4 | Human (Identities = 100%, Positives = 100%)Mouse (Identities = 100%, Positives = 100%) Rat (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:10000 | |
DNAJB11 Antibody | Q9UBS4 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 | |
RPAB1 Antibody | P19388 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:40000 | |
POLR1C Antibody | O15160 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 92%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
RPC8 Antibody | Q9Y535 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:40000 | |
RPC5 Antibody | Q9NVU0 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 92%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000 | |
RPC4 Antibody | P05423 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 92%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
RPC3 Antibody | Q9BUI4 | Human (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
RPC2 Antibody | Q9NW08 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:20000 | |
RPC1 Antibody | O14802 | Human | WB: 1:500~1:1000 ELISA: 1:5000 | |
POLR2J Antibody | P52435 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:10000 |
热门标签:小鼠热休克蛋白60 Hsp-60 ELISA 小鼠热休克蛋白60试剂盒