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小鼠低氧诱导因子1α(HIF-1α)ELISA试剂盒*新闻快讯
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小鼠低氧诱导因子1α(HIF-1α)elisa试剂盒*新闻快讯样品收集、处理及保存方法
1. 血清:使用不含热原和内毒素的试管,操作过程中避免任何细胞刺激,收集血液后,3000转离心10分钟将血清和红细胞迅速小心地分离。
2. 血浆:EDTA、柠檬酸盐或肝素抗凝。3000转离心30分钟取上清。
3. 细胞上清液:3000转离心10分钟去除颗粒和聚合物。
4. 组织匀浆:将组织加入适量盐水捣碎。3000转离心10分钟取上清。
5. 保存:如果样本收集后不及时检测,请按一次用量分装,冻存于-20℃,避免反复冻融,在室温下解冻并确保样品均匀地充分解冻。
自备物品
1.酶标仪(450nm)
2.高精度加样器及枪头:0.5-10uL、2-20uL、20-200uL、200-1000uL
3.37℃恒温箱
小鼠低氧诱导因子1α(HIF-1α)ELISA试剂盒*新闻快讯操作注意事项
1.试剂盒保存在2-8℃,使用前室温平衡20分钟。从冰箱取出的浓缩洗涤液会有结晶,这属于正常现象,水浴加热使结晶完全溶解后再使用。
2.实验中不用的板条应立即放回自封袋中,密封(低温干燥)保存。
3.标准品稀释液即可视为阴性对照或者空白;预处理后的样本无需稀释,直接取10μL加样即可。
4.严格按照说明书中标明的时间、加液量及顺序进行温育操作。
5.所有液体组分使用前充分摇匀。
小鼠低氧诱导因子1α(HIF-1α)ELISA试剂盒*新闻快讯相关产品发布:
I-TRAF Antibody | Q92844 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:10000 | |
T3JAM Antibody | Q9Y228 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000 | |
Serpin B5 Antibody | P36952 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:20000 | |
RAD23A Antibody | P54725 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:20000 | |
RAD54 Antibody | Q92698 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:1000 | |
RAD50 Antibody | Q92878 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:20000 | |
RAD21 Antibody | O60216 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:1000 | |
RAD51L3 Antibody | O75771 | Human | WB: 1:500~1:1000 ELISA: 1:20000 | |
RAD51L1 Antibody | O15315 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000 | |
PRPF19 Antibody | Q9UMS4 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:40000 | |
PMS2 Antibody | P54278 | Human | WB: 1:500~1:1000 ELISA: 1:10000 | |
PMS1 Antibody | P54277 | Human (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:10000 | |
PHLDA3 Antibody | Q9Y5J5 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:40000 | |
PHLA1 Antibody | Q8WV24 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:10000 | |
PEA15 Antibody | Q15121 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 92%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IF: 1:100~1:500 ELISA: 1:1000 | |
GPR132 Antibody | Q9UNW8 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:5000 | |
PARP4 Antibody | Q9UKK3 | Human (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:10000 | |
PARP3 Antibody | Q9Y6F1 | Human (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:1000 | |
PARP2 Antibody | Q9UGN5 | Human (Identities = 100%, Positives = 100%)Mouse (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000 | |
PARK7 Antibody | Q99497 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:20000 | |
NF1 Antibody | P21359 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000 | |
NBN Antibody | O60934 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:20000 | |
MUTYH Antibody | Q9UIF7 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000 | |
MSH6 Antibody | P52701 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:20000 | |
MSH3 Antibody | P20585 | Human | WB: 1:500~1:1000 ELISA: 1:20000 | |
MSH2 Antibody | P43246 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 92%, Positives = 92%);Rat (Identities = 92%, Positives = 92%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 | |
MRE11A Antibody | P49959 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:10000 | |
MLH3 Antibody | Q9UHC1 | Human | WB: 1:500~1:1000 ELISA: 1:40000 | |
MLH1 Antibody | P40692 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:20000 | |
MIPT3 Antibody | Q8TDR0 | Human (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 | |
MGMT Antibody | P16455 | Human, Rat | WB: 1:500~1:1000 ELISA: 1:5000 | |
MEN1 Antibody | O00255 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 | |
MALT1 Antibody | Q9UDY8 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:10000 | |
LEG9 Antibody | O00182 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000 | |
LEG8 Antibody | O00214 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 | |
LEG7 Antibody | P47929 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:5000 | |
LEG4 Antibody | P56470 | Human | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 | |
LEG2 Antibody | P05162 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 81%, Positives = 100%);Rat (Identities = 80%, Positives = 93%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
LEG1 Antibody | P09382 | Human; Rat | WB: 1:500~1:1000 ELISA: 1:40000 | |
LAMC3 Antibody | Q9Y6N6 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 85%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000 | |
LAMC1 Antibody | P11047 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:40000 | |
LAMB3 Antibody | Q13751 | Human (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
LAMB2 Antibody | P55268 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:5000 | |
LAMB1 Antibody | P07942 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 | |
LAMA5 Antibody | O15230 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 92%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:20000 | |
LAMA4 Antibody | Q16363 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000 | |
LAMA3 Antibody | Q16787 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
LAMA2 Antibody | P24043 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 85%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 | |
LAMA1 Antibody | P25391 | Human (Identities = 100%, Positives = 100%) | IF: 1:100~1:500 ELISA: 1:40000 | |
XRCC5 Antibody | P13010 | Human | WB: 1:500~1:1000 ELISA: 1:40000 | |
EIF4G2 Antibody | P78344 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:20000 | |
GRAK Antibody | P49863 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 | |
GRAH Antibody | P20718 | Human | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000 | |
GRAA Antibody | P12544 | Human (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
GA45G Antibody | O95257 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 92%, Positives = 92%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
FAF1 Antibody | Q9UNN5 | Human (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:1000 | |
FADD Antibody | Q13158 | Human (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 | |
EXO1 Antibody | Q9UQ84 | Human, Mouse | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000 | |
ELOA3 Antibody | Q8NG57 | Human | WB: 1:500~1:1000 ELISA: 1:20000 | |
ELOA2 Antibody | Q8IYF1/Q8NG57 | Human (Identities = 100%, Positives = 100%) | IF: 1:100~1:500 ELISA: 1:1000 | |
ELOA1 Antibody | Q14241 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
DNL4 Antibody | P49917 | Human | WB: 1:500~1:1000 ELISA: 1:20000 | |
DNL1 Antibody | P18858 | Human | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 | |
DMC1 Antibody | Q14565 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 85%, Positives = 100%); | IHC: 1:50~1:100 ELISA: 1:10000 | |
DIDO1 Antibody | Q9BTC0 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:10000 | |
DFF-45 Antibody | O00273 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 | |
DCC Antibody | P43146 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IF: 1:100~1:500 ELISA: 1:20000 | |
DAXX Antibody | Q9UER7 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000 | |
CYB5 Antibody | P00167 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000 | |
CLUS Antibody | P10909 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 | |
CIDEB Antibody | Q9UHD4 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000 | |
BCLW Antibody | Q92843 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000 | |
BCL10 Antibody | O95999 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 85%, Positives = 85%);Rat (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000 | |
BAX Antibody | P55269 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
BAX Antibody | Q07815 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:20000 | |
BAX Antibody | Q07814 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 IHC: 1:50~1:100 ELISA: 1:20000 | |
BARD1 Antibody | Q99728 | Human | WB: 1:500~1:1000 ELISA: 1:5000 | |
AIFM1 Antibody | O95831 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:5000 | |
HER2 Antibody | P04626 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 | |
GPR126 Antibody | Q86SQ4 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%) | IF: 1:100~1:500 ELISA: 1:20000 | |
PRKAB1 Antibody | Q9Y478 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:10000 | |
APC8 Antibody | Q9UJX2 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:20000 | |
Cyclosome 1 Antibody | Q9H1A4 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%) | IF: 1:100~1:500 ELISA: 1:10000 | |
GPR120 Antibody | Q5NUL3 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000 | |
MAPK9 Antibody | P45984 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:10000 | |
GPR119 Antibody | Q8TDV5 | Human | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000 | |
GPR116 Antibody | Q8IZF2 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000 | |
Werner Syndrome Helicase (Phospho-Ser1141) Antibody | Q14191 | Human | WB: 1:500~1:1000 ELISA: 1:5000 | |
IFM3 Antibody | Q01628 | Human | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:40000 | |
Vitamin D Receptor (Ab-208) Antibody | P11473 | Human | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 | |
Vitamin D Receptor (Phospho-Ser208) Antibody | P11473 | Human | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000 | |
IFIT1 Antibody | P09914 | Human | WB: 1:500~1:1000 ELISA: 1:10000 | |
IBP7 Antibody | Q16270 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:40000 | |
Vimentin (Ab-56) Antibody | P08670 | Human | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 |