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小鼠钙通道阻滞剂(CCB)ELISA试剂盒*上海新闻
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品牌名称：$brandModel.Title(进口品牌)型号：48T/96T 原产地：中国大陆 发布时间：2018/6/12 10:42:50更新时间：2024/12/26 14:56:45
产品摘要：小鼠钙通道阻滞剂(CCB)ELISA试剂盒*上海新闻为今品化学技术（上海）有限公司主打产品，质量好,发货及时,灵敏度高，特异性强，重复性好，其他动物类试剂盒种属一应俱全,指标数千种。公司配有专业实验室可寄样免费代检测支持现场观摩,提供售前售中售后全方位服务，欢迎来电询价或在线咨询索取产品说明书~
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详细内容
小鼠钙通道阻滞剂(CCB)elisa试剂盒*上海新闻操作步骤:
1．从已平衡至室温的密封袋中取出试验所需板条，未用的板条和干燥剂请放回铝箔袋内压 实自封条，密封口袋，放回4℃。
2．空白孔加标准品和标本稀释液，其余相应孔中加标本或不同浓度标准品(100ul/孔)，用封板胶纸封住反应孔，36℃孵箱孵育90分钟。
3．提前20分钟准备生物素化抗体工作液。
4．洗板5次。
5．空白孔加生物素化抗体稀释液，其余孔加入生物素化抗体工作液(100ul/孔)。用新封板胶纸封住反应孔，36℃孵箱孵育60分钟。
6．提前20分钟准备酶结合物工作液。避光室温(22-25 ) ℃ 放置。
7．洗板5次。
8．空白孔加酶结合物稀释液，其余孔加入酶结合物工作液(100ul/孔)。用新封板胶纸封住反应孔，36℃孵箱，避光孵育30分钟。
9．打开酶标仪电源，预热仪器，设置好检测程序。
10．洗板5次。
11．加入显色底物(TMB)100ul/孔，避光36℃孵箱，避光孵育15分钟。
12．加入终止液100ul/孔, 混匀后即刻测量OD450值(3分钟内)。在仪器保存读数结果并打印一份纸质结果。
13．实验完毕后将未用完的试剂按规定的保存温度放回电冰箱保存至有效期结束。
建议保存酶标板框，以备下次或者今后试验使用。
小鼠钙通道阻滞剂(CCB)ELISA试剂盒*上海新闻结果判断:
1. 每个标准品和标本的OD值应减去空白孔的OD值。
2. 手工绘制标准曲线。以标准品浓度作横坐标，OD值作纵坐标，以平滑线连接各标准品的坐标点。通过标本的OD值可在标准曲线上查出其浓度。
3. 若标本OD值高于标准曲线上限，应适当稀释后重测，计算浓度时应乘以稀释倍数。
小鼠钙通道阻滞剂(CCB)ELISA试剂盒*上海新闻其他相关产品推荐：
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| 100μg | CK2-β (Ab-209) Antibody | P67870 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg | CKII-β (Phospho-Ser209) Antibody | P05412 | Human, Mouse, Rat | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 | |
| 100μg | c-Jun (Ab-249) Antibody | P05412 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 | |
| 100μg | c-Jun (Ab-231) Antibody | P05412 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
| 100μg | c-Jun (Phospho-Thr231) Antibody | P05412 | Human, Mouse, Rat | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 | |
| 100μg | CHOP (Ab-30) Antibody | P35638 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
| 100μg | CHOP (Phospho-Ser30) Antibody | P35638 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 | |
| 100μg | Chk1 (Ab-301) Antibody | O14757 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 88%, Positives = 100%);Rat (Identities = 88%, Positives = 100%) | IF: 1:100~1:500 ELISA: 1:5000 | |
| 100μg | Chk1 (Phospho-Ser301) Antibody | O14757 | Human | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:40000 | |
| 100μg | Chk1 (Ab-286) Antibody | O14757 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000 | |
| 100μg | Chk1 (Phospho-Ser286) Antibody | O14757 | Human | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000 | |
| 100μg | CFTR (Ab-737) Antibody | P13569 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg | EDG4 Antibody | Q9HBW0 | Human | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000 | |
| 100μg | Centromeric Protein A (Ab-7) Antibody | P49450 | Human (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 | |
| 100μg | Centromeric Protein A (Phospho-Ser7) Antibody | P49450 | Human | IF: 1:100~1:500 ELISA: 1:10000 | |
| 100μg | CDK7 (Ab-170) Antibody | P50613 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 | |
| 100μg | CDK7 (Phospho-Thr170) Antibody | P50613 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 | |
| 100μg | CDC25B (Ab-353) Antibody | P30305 | Human | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
| 100μg | CDC25B (Phospho-Ser353) Antibody | P30305 | Human | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000 | |
| 100μg | CDC25A (Ab-124) Antibody | P30304 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:1000 | |
| 100μg | CDC25A (Phospho-Ser124) Antibody | P30304 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:5000 | |
| 100μg | CD88/C5aR (Ab-338) Antibody | P21730 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
| 100μg | CD88/C5aR (Phospho-Ser338) Antibody | P21730 | Human, Mouse | IHC: 1:50~1:100 ELISA: 1:40000 | |
| 100μg | CD71/TfR (Ab-24) Antibody | P02786 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 | |
| 100μg | CD71/TfR (Phospho-Ser24) Antibody | P02786 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 | |
| 100μg | CD50/ICAM-3 (Ab-518) Antibody | P32942 | Human | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000 | |
| 100μg | CD50/ICAM-3 (Phospho-Ser518) Antibody | P32942 | Human (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
| 100μg | EDG3 Antibody | Q99500 | Human | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000 | |
| 100μg | CD5 (Ab-Tyr453) Antibody | P06127 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:20000 | |
| 100μg | CD5 (Phospho-Tyr453) Antibody | P06127 | Human, Mouse | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000 | |
| 100μg | CD45 Antibody | P08575 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 88%, Positives = 88%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
| 100μg | CD45 (Phospho-Ser1007) Antibody | P08575 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000 | |
| 100μg | CD44 (Ab-706) Antibody | P16070 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg | CD44 (Phospho-Ser706) Antibody | P16070 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:20000 | |
| 100μg | CD4 (Ab-433) Antibody | P16070 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg | CD4 (Phospho-Ser433) Antibody | P01730 | Human | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000 | |
| 100μg | CD28 (Phospho-Tyr218) Antibody | P10747 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:40000 | |
| 100μg | CD227/Mucin 1 (Ab-1243) Antibody | P15941 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
| 100μg | CD227/Mucin 1 (Phospho-Tyr1243) Antibody | P15941 | Human, Mouse | IHC: 1:50~1:100 ELISA: 1:20000 | |
| 100μg | CD226/DNAM-1 (Ab-329) Antibody | Q15762 | Human, Mouse | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000 | |
| 100μg | CD226/DNAM-1(Phospho-Ser329)Antibody | Q15762 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000 | |
| 100μg | CD18/ITGB2 Antibody | P05107 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IF: 1:100~1:500 ELISA: 1:1000 | |
| 100μg | CD18/ITGB2 (Phospho-Thr758) Antibody | P05107 | Human; Mouse; Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:40000 | |
| 100μg | CD171/N-CAML1 (Ab-1181) Antibody | P32004 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000 | |
| 100μg | CD171/N-CAML1 (Phospho-Ser1181) Antibody | P32004 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:20000 | |
| 100μg | EDG2 Antibody | Q92633 | Human, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
| 100μg | CD136/RON (Phospho-Ser1394) Antibody | Q04912 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:40000 | |
| 100μl | CD130/gp130 (Ab-782) Antibody | P40189 | Human, Mouse | WB: 1:500~1:3000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 | |
| 100μg | Catenin-β (Ab-654) Antibody | P35222 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:40000 | |
| 100μg | Catenin-β (Phospho-Tyr654) Antibody | P35222 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:40000 | |
| 100μg | Catalase (Ab-385) Antibody | P04040 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:20000 | |
| 100μg | Catalase (Phospho-Tyr385) Antibody | P04040 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 | |
| 100μg | Caspase 3 (Ab-150) Antibody | P42574 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg | Caspase 3 (Phospho-Ser150) Antibody | P42574 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000 | |
| 100μg | Caspase 9 (Ab-196) Antibody | P55211 | Human | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000 | |
| 100μg | Caspase 9 (Phospho-Ser196) Antibody | P55211 | Human | WB: 1:500~1:1000 ELISA: 1:20000 | |
| 100μg | Caspase 9 (Ab-144) Antibody | P55211 | Human | WB: 1:500~1:1000 ELISA: 1:5000 | |
| 100μg | Caspase 9 (Phospho-Ser144) Antibody | P55211 | Human | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 | |
| 100μg | Caspase 9 (Ab-153) Antibody | P55211 | Human, Mouse, Rat | WB: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
| 100μg | Caspase 9 (Phospho-Tyr153) Antibody | P55211 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000 | |
| 100μg | CaMK4 (Ab-196/200) Antibody | Q16566 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
| 100μg | CaMK4 (Phospho-Thr196/200) Antibody | Q16566 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000 | |
| 100μg | CaMK1-α (Ab-177) Antibody | Q14012 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg | CaMK1-α (Phospho-Thr177) Antibody | Q14012 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
| 100μg | EDG1 Antibody | P21453 | Human | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000 | |
| 100μg | Calmodulin (Ab-79/81) Antibody | P62158 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
| 100μg | Calmodulin (Phospho-Thr79+Ser81) Antibody | P62158 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
| 100μg | Calcium Sensing Receptor (Ab-888) Antibody | P41180 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
| 100μg | Calcium Sensing Receptor (Phospho-Thr888) Antibody | P41180 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:40000 | |
| 100μg | C/EBP-ε (Ab-74) Antibody | Q15744 | Human | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
| 100μg | C/EBP-ε (Phospho-Thr74) Antibody | Q15744 | Human | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 | |
| 100μg | C/EBP-α (Ab-222) Antibody | P49715 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:1000 | |
| 100μg | C/EBP-α (Phospho-Thr226) Antibody | P49715 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:10000 | |
| 100μg | B-Myb (Ab-577/581) Antibody | P10244 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000 |
热门标签：小鼠钙通道阻滞剂 CCB ELISA 小鼠钙通道阻滞剂试剂盒
