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小鼠骨形成蛋白(BMPs)ELISA试剂盒*上海新闻
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详细内容
小鼠骨形成蛋白(BMPs)elisa试剂盒*上海新闻液体类标本:
包括血清、血浆、尿液、胸腹水、脑脊液、细胞培养上清等。
1)血清:
室温血液自然凝固10-20分钟后,离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。
2)血浆:
应根据标本的要求选择EDTA、柠檬酸钠或肝素作为抗凝剂,混合10-20分钟后,离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。
3)尿液:
用无菌管收集。离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。胸腹水、脑脊液参照此实行。
4)细胞培养上清:
A、检测分泌性的成份时,用无菌管收集。离心20分钟左右(2000-3000转/ 分)。仔细收集上清。
B、检测细胞内的成份时,用PBS(PH7.2-7.4)稀释细胞悬液,细胞浓度达到100万/ml左右。通过反复冻融,以使细胞破坏并放出细胞内成份。离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。
5)组织标本:
切割标本后,称取重量。加入一定量的PBS,PH7.4。用液氮迅速冷冻保存备用。标本融化后仍然保持2-8℃的温度。加入一定量的 PBS(PH7.4),用手工或匀浆器将标本匀浆充分。离心20分钟左右(2000-3000转/分)。仔细收集上清。分装后一份待检测,其余冷冻备用
规格:48T/96T(凡购买本公司试剂盒提供免费代测服务)
Elisa试剂盒报价,Elisa试剂盒价格,Elisa试剂盒说明书,Elisa试剂盒技术,Elisa试剂盒售后,Elisa试剂盒代测详情咨询
1.准确性:标准品线性回归与预期浓度相关系数R值,大于等于0.9900。
2.灵敏度:检测浓度小于10 pg/ml。
3.特异性:不与其它可溶性结构类似物交叉反应。
4.重复性:板内、板间变异系数均小于15%。
贮藏:2-8℃,避光防潮保存。
有效期:6个月
1.请每次测定的同时做标准曲线,做复孔。如标本中待测物质含量过高(样本OD值大于标准品孔孔的OD值),请先用样品稀释液稀释一定倍数(n倍)后再测定,计算时请*后乘以总稀释倍数(×n×5)。
2.封板膜只限一次性使用,以避免交叉污染。
3.底物请避光保存。
4.严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准。
100μg | Ataxin 1 Antibody | P54253 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 88%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
100μg | Ataxin 1 (Phospho-Ser776) Antibody | P54253 | Human, Mouse | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000 | |
100μg | A-RAF (Ab-301/302) Antibody | P10398 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 | |
100μg | A-RAF (Phospho-Tyr302) Antibody | P10398 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 | |
100μg | DRD1 Antibody | P21728 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 84%, Positives = 84%);Rat (Identities = 84%, Positives = 84%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
100μg | Arachidonate 5 Lipoxygenase (Ab-271) Antibody | P09917 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 | |
100μg | Arachidonate 5 Lipoxygenase (Phospho-Ser271) Antibody | P09917 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 | |
100μg | Aquaporin 2 (Ab-256) Antibody | P41181 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 | |
100μg | Aquaporin 2 (Phospho-Ser256) Antibody | P41181 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:40000 | |
100μg | Androgen Receptor (Ab-94) Antibody | P10275 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 88%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000 | |
100μg | Androgen Receptor (Phospho-Ser94) Antibody | P10275 | Human, Mouse | IF: 1:100~1:500 ELISA: 1:5000 | |
100μg | AMPK β1 (Ab-181) Antibody | Q9Y478 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000 | |
100μg | AMPK β1 (Phospho-Ser181) Antibody | Q9Y478 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 | |
100μg | AhR (Ab-36) Antibody | P35869 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
100μg | AhR (Phospho-Ser36) Antibody | P35869/A9YTQ3 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
100μg | Adrenergic Receptor B2 (Phospho-Ser355+Ser356) Antibody | P07550 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
100μg | ADAM 17 Antibody | P78536 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%)Rat (Identities = 100%, Positives = 100%) | IF: 1:100~1:500 ELISA: 1:1000 | |
100μg | ADAM 17 (Phospho-Thr735) Antibody | P78536 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:20000 | |
100μg | Actin-pan (α/β/γ) (Ab-53/55) Antibody | P60709/P63261 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 | |
100μg | Actin-pan (α/γ) (Phospho-Tyr55/53) Antibody | P60709/P63261 | Human, Mouse | IHC: 1:50~1:100 ELISA: 1:5000 | |
100μg | PFKFB2 (Ab-483) Antibody | O60825 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
100μg | PFKFB2 (Phospho-Ser483) Antibody | O60825 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000 | |
100μg | 53BP1 (Ab-6) Antibody | Q12888 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000 | |
100μg | 53BP1 (Phospho-Ser6) Antibody | Q12888 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
100μg | CYSLTR2 Antibody | Q9NS75 | Human | WB: 1:500~1:1000 ELISA: 1:20000 | |
100μg | 14-3-3 ζ/δ (Ab-232) Antibody | P63104 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 | |
100μg | 14-3-3 ζ/δ (Phospho-Thr232) Antibody | P63104 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 | |
100μg | 14-3-3 θ/τ (Ab-232) Antibody | P27348 | Human, Mouse | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000 | |
100μg | 14-3-3 θ/τ (Phospho-Ser232) Antibody | P27348 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
100μg | YAP (Ab-127) Antibody | P46937 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:1000 | |
100μg | YAP (Phospho-Ser127) Antibody | P46937 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000 | |
100μg | CYSLTR1 Antibody | Q9Y271 | Human | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000 | |
100μg | TLK1 Antibody | Q9Y4F6 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%) | IF: 1:100~1:500 ELISA: 1:5000 | |
100μg | TIE2 Antibody | Q02763 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:5000 | |
100μg | TIE2 (Phospho-Tyr1102) Antibody | Q02763 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:40000 | |
100μg | CRHR2 Antibody | Q13324 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000 | |
100μg | SHIP1 (Ab-1020) Antibody | Q92835 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:5000 | |
100μg | SHIP1 (Phospho-Tyr1021) Antibody | Q92835 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:40000 | |
100μg | SGK (Ab-78) Antibody | O00141 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
100μg | SGK (Phospho-Ser78) Antibody | O00141 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:10000 | |
100μg | CRHR1 Antibody | P34998 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000 | |
100μg | Ras-GRF1 (Ab-916) Antibody | Q13972 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 88%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:1000 | |
100μg | Ras-GRF1 (Phospho-Ser916) Antibody | Q13972 | Mouse, Rat | IHC: 1:50~1:100 ELISA: 1:20000 | |
100μg | PP1-α (Ab-320) Antibody | P62136 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
100μg | PP1-α (Phospho-Thr320) Antibody | P62136 | Human, Mouse, Rat | IHC: 1:50~1:100 ELISA: 1:10000 | |
100μg | PLD1 Antibody | Q13393 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:1000 | |
100μg | PLD1 (Phospho-Ser561) Antibody | Q13393 | Human, Mouse, Rat | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
100μg | PLC β3 Antibody | Q01970 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 88%, Positives = 100%);Rat (Identities = 88%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
100μg | PLC β3 (Phospho-Ser537) Antibody | Q01970 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 | |
100μg | PKD1/PKC μ (Ab-910) Antibody | Q15139 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:5000 | |
100μg | PKD1/PKC μ (Phospho-Ser910) Antibody | Q15139 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:10000 | |
100μg | PKD1/2/3/PKC μ (Ab-744/748) Antibody | Q15139 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:1000 | |
100μg | PKD1/2/3/PKC μ (Phospho-Ser738+Ser742) Antibody | Q15139 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
100μg | PKC θ (Ab-538) Antibody | Q04759 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
100μg | PKC θ (Phospho-Thr538) Antibody | Q04759 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
100μg | PKC δ (Phospho-Tyr313) Antibody | Q05655 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 | |
100μg | PKC δ (Ab-505) Antibody | Q05655 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
100μg | PKC δ (Phospho-Thr505) Antibody | Q05655 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 | |
100μg | PKC α (Ab-638) Antibody | P17252 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
100μg | PKC α (Phospho-Thr638) Antibody | P17252 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 | |
100μg | Pin1 (Ab-1619) Antibody | Q13526 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
100μg | Pin1 (Phospho-Ser16) Antibody | Q13526 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 | |
100μg | Pim-1 (Ab-309) Antibody | P06803 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
100μg | Pim-1 (Phospho-Tyr309) Antibody | P06803 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:40000 | |
100μg | PEA-15 (Ab-104) Antibody | Q15121 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%)Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:1000 | |
100μg | PEA-15 (Phospho-Ser104) Antibody | Q15121 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 | |
100μg | PBK/TOPK (Ab-9) Antibody | Q96KB5 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:000 | |
100μg | PBK/TOPK (Phospho-Thr9) Antibody | Q96KB5 | Human | WB: 1:500~1:1000 ELISA: 1:40000 | |
100μg | CLPT1 Antibody | O96005 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:10000 |
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