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小鼠幽门螺旋菌IgG(Hp-IgG)ELISA试剂盒*上海新闻
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详细内容
小鼠幽门螺旋菌IgG(Hp-IgG)elisa试剂盒*上海新闻标本收集注意事项:
在收集标本前都必须有一个完整的计划,必须清楚要检测的成份是否足够稳定。
2)我们提倡新鲜标本尽早检测,对收集后及时进行检测。
1.检测原理:采用双抗体夹心ABC-ELISA法
2.试剂盒保存:2-8℃(频繁使用时);-20℃(较长时间不用时)。
3.刚开启的酶联板孔中可能会含有少许水样物质,此为正常现象,不会对实验结果造成任何影响。
4.中、英文说明书可能会有不一致之处,请以英文说明书为准。
5.浓洗涤液低温保存会有盐析出,稀释时可在水浴中加温助溶。
6. 不要用其它生产厂家的试剂替换试剂盒中的试剂。
间接法是检测抗体常用的方法。其原理为利用酶标记的抗抗体(抗人免疫球蛋白抗体)以检测与固相抗原结合的受检抗体,故称为间接法操作步骤如下:
1)将特异性抗原与固相载体联结,形成固相抗原。洗涤除去未结合的抗原及杂质。
2)加稀释的受检血清,保温反应。血清中的特异抗体与固相抗原结合,形成固相抗原抗体复合物。经洗涤后,固相载体上只留下特异性抗体,血清中的其他成份在洗涤过程中被洗去。
3)加酶标抗抗体。可用酶标抗人Ig以检测总抗体,但一般多用酶标抗人IgG检测IgG抗体。固相免疫复合物中的抗体与酶标抗体抗体结合,从而间接地标记上酶。洗涤后,固相载体上的酶量与标本中受检抗体的量正相关。
4)加底物显色
小鼠幽门螺旋菌IgG(Hp-IgG)ELISA试剂盒*上海新闻主要规格/保存方式:
规格:48T/96T,可以提供定量和定性。
2-8℃(频繁使用时);-20℃(较长时间不用时)。
公司致力于建立并完善供货系统为广大科研单位提供免疫学,分子学,蛋白质表达纯化及检测,细胞学相关实验等。主营产品:Elisa试剂盒、生物试剂、化学试剂、标准品对照品、培养基、抗体、血清、免疫组化试剂盒、生化试剂。(凡够买我公司试剂盒可免费提供代测服务。)敬请您的来电询购。
主要用于科研使用,不得用于医学诊断.使用前仔细阅读本说明书。
小鼠幽门螺旋菌IgG(Hp-IgG)ELISA试剂盒*上海新闻其他相关产品发布:
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100μg | Csk (Ab-364) Antibody | P41240 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 | |
100μg | Csk (Phospho-Ser364) Antibody | P41240 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:1000 | |
100μg/100μl | Chk2 (Ab-387) Antibody | O96017 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
100μg/100μl | Chk2 (Phospho-Thr387) Antibody | O96017 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~100 ELISA: 1:5000 | |
100μg/100μl | CDC6 (Ab-54) Antibody | Q99741 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
100μg/100μl | CDC6 (Phospho-Ser54) Antibody | Q99741 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~100 ELISA: 1:5000 | |
100μg | CD19 (Ab-531) Antibody | P15391 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:1000 | |
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