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小鼠组织蛋白去乙酰化酶(HD)ELISA试剂盒*上海新闻
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小鼠组织蛋白去乙酰化酶(HD)elisa试剂盒*上海新闻仅供研究使用。
检测范围: 96T,来电详询。
用于测定人血清、血浆及相关液体样本中指标含量。
小鼠组织蛋白去乙酰化酶(HD)ELISA试剂盒*上海新闻组成
1 30 倍浓缩洗涤液 20ml×1 瓶 7 终止液 6ml×1 瓶
2 酶标试剂 6ml×1 瓶 8 标准品(8μg/L) 0.5ml×1 瓶
3 酶标包被板 12 孔×8 条 9 标准品稀释液 1.5ml×1 瓶
4 样品稀释液 6ml×1 瓶 10 说明书 1 份
5 显色剂A 液 6ml×1 瓶 11 封板膜 2 张
6 显色剂B 液 6ml×1/瓶 12 密封袋 1 个
标本要求
1.标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验。若不能
马上进行试验,可将标本放于-20℃保存,但应避免反复冻融
2.不能检测含NaN3 的样品,因NaN3 抑制辣根过氧化物酶的(HRP)活性。
小鼠组织蛋白去乙酰化酶(HD)ELISA试剂盒*上海新闻操作步骤
1. 标准品的稀释:本试剂盒提供原倍标准品一支,用户可按照下列图表在小试管中进行稀
释。
4μg/L 5 号标准品 150μl 的原倍标准品加入150μl 标准品稀释液
2μg/L 4 号标准品 150μl 的5 号标准品加入150μl 标准品稀释液
1μg/L 3 号标准品 150μl 的4 号标准品加入150μl 标准品稀释液
0.5μg/L 2 号标准品 150μl 的3 号标准品加入150μl 标准品稀释液
0.25μg/L 1 号标准品 150μl 的2 号标准品加入150μl 标准品稀释液
2. 加样:分别设空白孔(空白对照孔不加样品及酶标试剂,其余各步操作相同)、标准孔、
待测样品孔。在酶标包被板上标准品准确加样50μl,待测样品孔中先加样品稀释液40μl,
然后再加待测样品10μl(样品*终稀释度为5 倍)。加样将样品加于酶标板孔底部,尽
量不触及孔壁,轻轻晃动混匀。
3. 温育:用封板膜封板后置37℃温育30 分钟。
4. 配液:将30 倍浓缩洗涤液用蒸馏水30 倍稀释后备用
5. 洗涤:小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30 秒后弃去,如此
重复5 次,拍干。
6. 加酶:每孔加入酶标试剂50μl,空白孔除外。
7. 温育:操作同3。
8. 洗涤:操作同5。
9. 显色:每孔先加入显色剂A50μl,再加入显色剂B50μl,轻轻震荡混匀,37℃避光显色
10 分钟.
10. 终止:每孔加终止液50μl,终止反应(此时蓝色立转黄色)。
11. 测定:以空白空调零,450nm 波长依序测量各孔的吸光度(OD 值)。 测定应在加终止
液后15 分钟以内进行。
小鼠组织蛋白去乙酰化酶(HD)ELISA试剂盒*上海新闻更多热销产品推荐:
n-NOS (Phospho-Ser852) Antibody | P29476 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
NMDAR2B (Ab-1474) Antibody | Q13224 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:1000 | |
NMDAR2B (Phospho-Tyr1474) Antibody | Q01097 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
NF-κB p65 (Ab-505) Antibody | Q04206 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:40000 | |
NF-κB p65 (Phospho-Thr505) Antibody | Q04206 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 92%, Positives = 100%);Rat (Identities = 92%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:1000 | |
NF-κB p105/p50 Antibody | P19838 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 92%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:1000 | |
NFAT4 (Ab-165) Antibody | Q12968 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:10000 | |
NFAT4 (Phospho-Ser165) Antibody | Q12968 | Human, Mouse | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000 | |
NFAT3 (Ab-676) Antibody | Q14934 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
NFAT3 (Phospho-Ser676) Antibody | Q14934 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:1000 | |
NFAT2 (Phospho-Ser294) Antibody | O95644 | Human, Mouse | IHC: 1:50~1:100 ELISA: 1:1000 | |
MYPT1 (Ab-853) Antibody | O14974 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
MYPT1 (Phospho-Thr853) Antibody | O14974 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
MYPT1 (Ab-696) Antibody | O14974 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
MYPT1 (Phospho-Thr696) Antibody | O14974 | Human, Mouse, Rat | IHC: 1:50~1:100 IF: 1:500~1:1000 ELISA: 1:1000 | |
MYOD (Phospho-Ser200) Antibody | P15172 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:5000 | |
MYB (Ab-12) Antibody | P10242 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 | |
MYB (Phospho-Ser12) Antibody | P10242 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
MUNC-18a (Ab-313) Antibody | P61764 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000 | |
MUNC-18a (Phospho-Ser313) Antibody | P61764 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:1000 | |
MRLC2 Antibody | P19105 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 92%, Positives = 92%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
MITF (Ab-180/73) Antibody | O75030 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 | |
MITF (Phospho-Ser180/73) Antibody | O75030 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:1000 | |
Met (Phospho-Tyr1356) Antibody | Q09YK0 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:10000 | |
MEF2D Antibody | Q14814 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:1000 | |
MEF2D (Phospho-Ser444) Antibody | Q14814 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 | |
MEF2C (Phospho-Ser387) Antibody | Q06413 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 92%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:10000 | |
MDM2 (Ab-166) Antibody | Q00987 | Human, Mouse | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000 | |
MDM2 (Phospho-Ser166) Antibody | Q00987 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
M-CSF Receptor (Ab-809) Antibody | P07333 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:10000 | |
M-CSF Receptor (Phospho-Tyr809) Antibody | P07333 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:1000 | |
Lck (Ab-192) Antibody | P06239 | Human, Mouse | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000 | |
Lck (Phospho-Tyr192) Antibody | P06239 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:10000 | |
Lck (Ab-505) Antibody | P06239 | Human, Mouse | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000 | |
Lck (Phospho-Tyr505) Antibody | P06239 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:60000 | |
LAT (Ab-191) Antibody | O43561 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:5000 | |
LAT (Phospho-Tyr191) Antibody | O43561 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
Lamin A/B (Ab-392) Antibody | P02545 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
Lamin A/C (Phospho-Ser392) Antibody | P02545 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
KIT (Phospho-Tyr703) Antibody | P10721 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
KIT (Ab-936) Antibody | P10721 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:5000 | |
KIT (Phospho-Tyr936) Antibody | P10721 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
JNK1/2/3 (Ab-183/185) Antibody | P45983/P45984/P53779 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000 | |
JNK1/2/3 (Phospho-Thr183+Tyr185) Antibody | P45983/P45984/P53779 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:10000 | |
JAK2 (Ab-570) Antibody | O60674 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000 | |
JAK2 (Phospho-Tyr570) Antibody | O60674 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:1000 | |
IRS-1 Antibody | P35568 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
IRS-1 (Phospho-Ser323) Antibody | P35568 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 | |
IRF3 (Ab-385) Antibody | Q14653 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
IRF-3 (Phospho-Ser385) Antibody | Q14653 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 | |
IRAK1 (Ab-387) Antibody | P51617 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:1000 | |
IRAK1 (Phospho-Thr387) Antibody | P51617 | Human, Mouse, Rat | IF: 1:100~1:500 ELISA: 1:1000 | |
IR (Ab-1361) Antibody | P06213 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:1000 | |
IR (Phospho-Tyr1361) Antibody | P06213 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 | |
IR (Ab-1355) Antibody | P06213 | Human (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
IR (Phospho-Tyr1355) Antibody | P06213 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 | |
IR Antibody | P06213 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IF: 1:100~1:500 ELISA: 1:1000 | |
Integrin β1 (Ab-789) Antibody | P05556 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:1000 | |
Integrin β1 (Phospho-Thr789) Antibody | P05556 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
IKK-β Antibody | O14920 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:10000 | |
IKK-β (Phospho-Tyr199) Antibody | O14920 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
HSP20 Antibody | O14558 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:5000 | |
HSP20 (Phospho-Ser16) Antibody | O14558 | Human, Mouse, Rat | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
GRK2 Antibody | P25098 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:1000 | |
GRK2 (Phospho-Ser29) Antibody | P25098 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 | |
FOXO1A/3A Antibody | Q12778 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:5000 | |
FOXO1A/3A (Phospho-Ser322+Ser325) Antibody | Q12778 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:1000 | |
FOXO1A (Ab-329) Antibody | Q12778 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
FOXO1A (Phospho-Ser329) Antibody | Q12778 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
FOXO1/3/4-pan (Ab-24/32) Antibody | Q12778/O43524/P98177 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:1000 | |
FOXO1/3/4-pan (Phospho-Thr24/32) Antibody | Q12778/O43524/P98177 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
FLT3 (Ab-599) Antibody | P36888 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:1000 | |
FLT3 (Phospho-Tyr599) Antibody | P36888 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:1000 | |
FGFR1 (Ab-654) Antibody | P11362 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000 | |
FGFR1 (Phospho-Tyr654) Antibody | P11362 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000 | |
FGFR1 (Ab-766) Antibody | P11362 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
FGFR1 (Phospho-Tyr766) Antibody | P11362 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
FAK (Ab-407) Antibody | Q05397 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
FAK (Phospho-Tyr407) Antibody | Q05397 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
ETS1 (Ab-38) Antibody | P27577 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
ETS1 (Phospho-Thr38) Antibody | P27577 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 | |
ERF Antibody | P50548 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 92%, Positives = 100%); | IHC: 1:50~1:100 IF: 1:500~1:1000 ELISA: 1:10000 | |
ERF (Phospho-Thr526) Antibody | P50548 | Human, Mouse | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 | |
Estrogen Receptor-α (Ab-537) Antibody | P03372 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
Estrogen Receptor-α (Phospho-Tyr537) Antibody | P03372 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
EGFR (Ab-1016) Antibody | P00533 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:2000 | |
CREB (Ab-121) Antibody | P16220 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
CREB (Phospho-Ser121) Antibody | P16220 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:5000 |
热门标签:小鼠组织蛋白去乙酰化酶 HD ELISA 小鼠组织蛋白去乙酰化酶试剂盒