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小鼠维生素K1(VK1)ELISA试剂盒*今品化学新闻
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小鼠维生素K1(VK1)elisa试剂盒*今品化学新闻特点:
1.专一性强。抗原与抗体的免疫反应是专一反应,而免疫酶技术以免疫反应为基础,所检测的对象是抗原(或抗体),使用的抗体除标记了酶以外,与普通抗体的免疫反应特性并无多大差别。
2.灵敏度高。由于抗体联结上了酶,因此,借助于酶与底物的显色反应,显示抗原与抗体的结合,大大提高了检测的灵敏性,使检测水平接近放射免疫测定法。
3.样品易保存。经过酶反应显示的有色产物大多比较稳定,因此有利于样品的保存。
4.结果易观察。对检测结果即可用肉眼观察,又可用显微镜观察,也可以用分光光度计进行比色测定,还可用显微镜观察。这是因为某些酶反应产物能使电子密度发生改变,从而引起被检测物的显示。
5.可以定量测定。溶液中的抗原物质,应用酶免吸附技术进行比色测定,依据光密度值的变化,可以定量。预计用细胞分光光度计可对组织内的抗原进行免疫酶技术的定量测定。
6.可以大规模测定样品。如有成千上万份样品需要进行检测,免疫酶技术都能在较短时间内完成。
7.仪器和试剂简单。对免疫没技术来说,不需要荧光显微镜,也不需要测定放射性的特殊仪器,所用仪器及试剂均属一般性仪器与试剂,普通实验室及生产应用单位均易购置
小鼠维生素K1(VK1)ELISA试剂盒*今品化学新闻液体类标本:包括血清、血浆、尿液、胸腹水、脑脊液、细胞培养上清等。1)血清:室温血液自然凝固10-20分钟后,离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。2)血浆:应根据标本的要求选择EDTA、柠檬酸钠或肝素作为抗凝剂,混合10-20分钟后,离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。3)尿液:用无菌管收集。离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。胸腹水、脑脊液参照此实行。4)细胞培养上清:A、检测分泌性的成份时,用无菌管收集。离心20分钟左右(2000-3000转/ 分)。仔细收集上清。B、检测细胞内的成份时,用PBS(PH7.2-7.4)稀释细胞悬液,细胞浓度达到100万/ml左右。通过反复冻融,以使细胞破坏并 放出细胞内成份。离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。5)组织标本:切割标本后,称取重量。加入一定量的PBS,PH7.4。用液氮迅速冷冻保存备用。标本融化后仍然保持2-8℃的温度。加入一定量的PBS(PH7.4),用手工或匀浆器将标本匀浆充分。离心20分钟左右(2000-3000转/分)。仔细收集上清。分装后一份待检测,其余冷冻备用。
小鼠维生素K1(VK1)ELISA试剂盒*今品化学新闻其他相关产品发布:
100μg/100μl | CREB (Phospho-Ser129) Antibody | P16220 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
100μg/100μl | Cortactin(Phospho-Tyr466) Antibody | Q14247 | Human, Mouse | WB: 1:500~1:1000 | |
100μg/100μl | Tau (Phospho-Ser422) Antibody | P10636 | Human, Mouse, Rat | WB:1:500~1000 | |
100μg100μl | p70 S6 Kinase(Phospho-Ser411) Antibody | P23443 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50-1:100 | |
100μg/100μl | Histone H2A.X (Phospho-Ser139) Antibody | P16104 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
100μg/100μl | synapsin (Phospho-Ser9) Antibody | P17600 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
100μg/100μl | Merlin (Phospho-Ser518) Antibody | P35240 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
100μg/100μl | MARCKS (Phospho-Ser162) Antibody | P29966 | Human, Mouse, Rat | IF:1:100~1:200 | |
100μg/100μl | EGFR (phospho-Tyr1110) Antibody | P00533 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
100μg/100μl | HSF1 (phospho-Ser303) Antibody | Q00613 | Human | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
100μg/100μl | GluR1(phospho-Ser849) Antibody | P42261 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
100μg/100μl | NFκB-p65((phospho-Ser311) Antibody | Q04206 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50-1:100 | |
100μg/100μl | Connexin 43 (phospho-Ser367) Antibody | P17302 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50-1:100 | |
100μg/100μl | Tau (Phospho-Thr212) Antibody | P10636 | Human, Mouse, Rat | WB:1:500~1000 | |
100μg/100μl | α-Synuclein (Phospho-Tyr125) Antibody | P37840 | Human | IF:1:100~1:200 | |
100μg/100μl | RelB (Phospho-Ser552) Antibody | Q01201 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50-1:100 | |
100μg/100μl | p70 S6 Kinase (Phospho-Thr421) Antibody | P23443 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50-1:100 | |
100μg/100μl | P38 MAPK(Phospho-Tyr182) Antibody | Q16539 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50-1:100 | |
100μg/100μl | P38 MAPK(Phospho-Thr180) Antibody | Q16539 | Human, Mouse, Rat | IHC: 1:50~1:100 | |
100μg/100μl | NFκB-p105/p50(Phospho-Ser932) Antibody | P19838 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
100μg/100μl | SAPK/JNK (Phospho-Thr183) Antibody | P45984; P53779 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
100μg/100μl | HSP27(Phospho-Ser82) Antibody | P04792 | Human | WB: 1:500~1:1000 IHC: 1:50-1:100 | |
100μg/100μl | HSP27(Phospho-Ser78) Antibody | P04792 | Human, | WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200 | |
100μg/100μl | p44/42 MAP Kinase (Phospho-Tyr204) Antibody | P27361 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100-200 | |
100μg/100μl | p44/42 MAP Kinase(Phospho-Thr202) Antibody | P27361 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50-1:100 IF:1:100~1:200 | |
100μg/100μl | CDC2 (Phospho-Tyr15) Antibody | P06493 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
100μg/100μl | BRCA1(Phospho-Ser1423) Antibody | P38398 | Human | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
100μg/100μl | c-Kit (Phospho-Tyr721) Antibody | P10721 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
100μg/100μl | Met (Phospho-Tyr1349) Antibody | P08581 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
100μg/100μl | BRCA1 (Phospho-Ser988) Antibody | P38398 | Human | IF:1:100~1:200 | |
100μg/100μl | IRS-1 (Phospho-Ser307) Antibody | P35568 | Human, Mouse, Rat | IHC: 1:50~1:100 | |
100μg/100μl | Stathmin 1 (Phospho-Ser15) Antibody | P16949 | Human, Mouse, Rat | IHC: 1:50~1:100 | |
100μg/100μl | eIF4E (Phospho-Ser209) Antibody | P06730 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
100μg/100μl | S6 Ribosomal Protein (Phospho-Ser235) Antibody | P62753 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
100μg/100μl | IRS-1 (Phospho-Ser639) Antibody | P35568 | Human, Mouse, Rat | IHC: 1:50~1:100 | |
100μg/100μl | IRS-1(Phospho-Ser636) Antibody | P35568 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50-1:100 | |
100μg/100μl | EGFR (Phospho-Tyr869) Antibody | P00533 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
100μg/100μl | EGFR (Phospho-Tyr1197) Antibody | P00533 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
100μg/100μl | Met (Phospho-Tyr1234) Antibody | P08581 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
100μg/100μl | Stathmin 1 (Phospho-Ser24) Antibody | P16949 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
100μg/100μl | 4E-BP1 (Phospho-Thr45) Antibody | Q13541 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
100μg/100μl | 4E-BP1 (Phospho-Thr36) Antibody | Q13541 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
100μg/100μl | mTOR(Phospho-Ser2448) Antibody | P42345 | Human, Mouse, Rat | IHC: 1:50~1:100 | |
100μg/100μl | EGFR(Phospho-Tyr1172) Antibody | P00533 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
100μg/100μl | β-Catenin (Phospho-Ser37) Antibody | P35222 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
100μg/100μl | β-Catenin (Phospho-Ser33) Antibody | P35222 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50-1:100 | |
100μg/100μl | NFκB-p65 (Phospho-Ser529) Antibody | Q04206 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
100μg/100μl | Pyk2 (Phospho-Tyr402) Antibody | Q14289 | Human, Mouse, Rat | WB:1:500~1:1000 IHC: 1:50~1:100 | |
100μg/100μl | FAK (Phospho-Tyr397) Antibody | Q05397 | Human, Mouse, Rat | WB:1:500~1000 | |
100μg/100μl | VASP (Phospho-Ser157) Antibody | P50552 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
100μg/100μl | IkB-ε (Phospho-Ser22) Antibody | O00221 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
100μg/100μl | Tyrosine Hydroxylase (Phospho-Ser40) Antibody | P07101 | Human, Mouse, Rat | IF:1:100~1:200 | |
100μg/100μl | Synaptotagmin II (Phospho-Thr202) Antibody | Q8N9I0 | Human, Mouse, Rat | IF:1:100~1:200 | |
100μg/100μl | Synaptotagmin (Phospho-Ser309) Antibody | P21579 | Human, Mouse, Rat | IF:1:100~1:200 | |
100μg/100μl | JAK2 (Phospho-Tyr221) Antibody | O60674 | Human, Mouse, Rat | IHC: 1:50~1:100 | |
100μg/100μl | p53 (Phospho-Ser46) Antibody | P04637 | Human | WB: 1:500~1:1000 | |
100μg/100μl | p53 (Phospho-Ser15) Antibody | P04637 | Human | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100-200 | |
100μg/100μl | VEGFR2 (Phospho-Tyr951) Antibody | P35968 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200 | |
100μg/100μl | PKD/PKCμ (Phospho-Ser738) Antibody | Q15139 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
100μg/100μl | Estrogen Receptor-α (Phospho-Ser104) Antibody | P03372 | Human, Mouse | IHC: 1:50~1:100 | |
100μg/100μl | STAT6 (Phospho-Tyr641) Antibody | P42226 | Human | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
100μg/100μl | ATF2 (Phospho-Ser112 or 94) Antibody | P15336 | Human, Mouse, Rat | IHC: 1:50~1:100 | |
100μg/100μl | c-Jun (Phospho-Ser243) Antibody | P05412 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
100μg/100μl | NFκB-p65 (Phospho-Ser536) Antibody | Q04206 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200 | |
100μg/100μl | p21Cip1 (Phospho-Thr145) Antibody | P38936 | Human | IHC: 1:50~1:100 | |
100μg/100μl | MEK1 (Phospho-Ser217) Antibody | Q02750 | Human, Mouse, Rat | IHC: 1:50~1:100 | |
100μg/100μl | Raf1 (Phospho-Ser338) Antibody | P04049 | Human, Mouse | WB:1:500~1000 | |
100μg/100μl | Rac1 (Phospho-Ser71) Antibody | P15154 | Human, Mouse, Rat | WB:1:500~1000 | |
100μg/100μl | Ezrin (Phospho-Thr566) Antibody | P15311 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
100μg/100μl | Paxillin (Phospho-Tyr31) Antibody | P49023 | Human | WB: 1:500~1:1000 | |
100μg/100μl | Bcr (Phospho-Tyr177) Antibody | P11274 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
100μg/100μl | SMC1(Phospho-Ser957) Antibody | Q14683 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50-1:100 | |
100μg/100μl | HSP90B (Phospho-Ser254) Antibody | P08238 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
100μg/100μl | HSF1 (Phospho-Ser307) Antibody | Q00613 | Human | WB: 1:500~1:1000 IF: 1:100-200 | |
100μg/100μl | HDAC5 (Phospho-Ser498) Antibody | Q9UQL6 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
100μg/100μl | HDAC4 (Phospho-Ser632) Antibody | P56524 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
100μg/100μl | HDAC2 (Phospho-Ser394) Antibody | Q92769 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100-200 | |
100μg/100μl | APP (Phospho-668) Antibody | P05067 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
100μg/100μl | EGFR (Phospho-Thr693) Antibody | P00533 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
100μg/100μl | EGFR (Phospho-Thr678) Antibody | P00533 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
100μg/100μl | DARPP-32 (Phospho-Thr75) Antibody | Q9UD71 | Human, Mouse, Rat | IF:1:100~1:200 | |
100μg/100μl | Histone H3.1 (Phospho-Ser10) Antibody | P68431 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100-200 | |
100μg/100μl | AMPK1 (Phospho-Thr174) Antibody | Q13131 | Human, Mouse, Rat | IHC: 1:50-1:100 | |
100μg/100μl | ADD1 (Phospho-Ser726) Antibody | P35611 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
100μg/100μl | 14-3-3 ζ (Phospho-Ser58) Antibody | P63104 | Human, Mouse, Rat | WB: 1:500-1000 IHC: 1:50-1:100 IF: 1:100-200 |
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