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小鼠细胞间粘附分子2(ICAM-2/CD102)ELISA试剂盒*新闻快讯
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详细内容
每套试剂盒中内容(96T)材料和数量:
标准品 96T(2vial)
预包被抗体的96孔板 96T(8×12)
生物素标记人ANG抗体 96T (1:100)
ABC(亲和素-过氧化物酶复合物) 96T (1:100)
样品稀释液 96T×2
抗体稀释液 96T×1
ABC稀释液 96T×1
TMB显色液A 96T×1
TMB显色液B 96T×1
TMB终止液 96T×1
ELISA专用洗涤液 96T×1(1:25)
【需要而未提供的试剂和器材】
1. 37℃恒温箱。
2. 标准规格酶标仪。
3. 自动洗板机。
4. 单通道或多通道可调节移液器以及其吸头。
5. 蒸馏水,容量瓶等
6. 干净的试管和Eppendof管。
【小鼠细胞间粘附分子2(ICAM-2/CD102)ELISA试剂盒*新闻快讯注意事项】
1. 从-20℃取出的试剂盒,在4℃解冻过夜。盒内每一瓶解冻的溶液在开启瓶盖之前都要轻轻摇匀,避免解冻时出现的分层,否则会出现浓度不均一的现象。
2. 开启试剂盒之前要室温平衡至少30分钟。
3. 配制各种试剂时,切记混匀!
4. 用户在初次使用试剂盒时,应将各种试剂管离心数分钟,以便试剂集中到管底。
5. 建议所有标准品、样本都做双份检测。
6. 要严格避免操作过程中酶标板干燥。干燥会使酶标板上生物成份迅速失活!
7. 为免交叉污染,要避免重复使用手中的吸头和试管。
【样品的准备和保存】
样品如果不立即分析,应分装后冷冻保存,且避免反复冻融。
血清——用干净试管收集血液,室温凝固2小时或4℃过夜,离心1000×g 10分钟,收集血清。立即分析或分装后-20℃冷冻保存。
细胞培养、组织匀浆、体液——离心去除沉淀,立即分析或分装后-20℃冷冻保存。
【样品稀释的一般原则】
用户须查阅文献了解标本内待测因子的含量,决定适当的稀释倍数,以使稀释后样品中待测因子的浓度处于ELISA试剂盒的检测范围。样品的稀释应有详细记录。
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