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小鼠血小板生成素(TPO)ELISA试剂盒*今品化学新闻
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详细内容
1. 抗原固定(包埋):将对应疫苗用0.05M的碳酸钠(Na2CO3)溶液稀释至2 微克/毫升, 在96孔ELISA薄板上每孔加100µL, 于37度2小时或4度冰箱过夜。第二天
2. 封闭(BLOCKING):用PBSt冲洗薄板三次,每次必须把孔中液体拍掉. 再在每孔加1%脱指奶粉或BSA或0.1%吐温(用PBSt稀释)100µL,37度下放置30分钟.
3. 用PBSt冲洗薄板3次,拍干孔中液体。
4. 滴度测试,每个样本用8孔测试。
(1)如样本为血清,则每孔加上100µL含5µg/mL抑制疫苗(用0.1%BSA的PBSt液稀释),抑制疫苗为偶联物和要检测抗体的疫苗的偶联物一样的另一种疫苗。
(2)如样本为已纯化的抗体,则不用加抑制疫苗。
5. (1)血清样本用含5µg/mL抑制疫苗溶液(用0.1%BSA的PBSt液稀释)稀释400倍.在孔加100µL(总量为200µL/孔),混匀后移液100µL到第二孔,同样混匀后从第二孔移液100µL到第三孔•••••如此直至第7孔,留第八孔为空白对照。这样从1-7孔的*终样本稀释度分别为:1:800,1:1600,1:3200, 1:6400, 1: 12800, 1: 25600, 1: 51200。(2)抗体样本则稀释1000倍,混匀后移液50µL到第二孔.....至第七孔,留第八孔为空白对照。
6. 稀释完后在37度反应60分钟.
7. 吸掉每孔中的液体,用PBSt分别清洗4次,每次拍干孔内液体.
8. 上二抗:将抗兔IgG HRP用PBSt稀释,稀释倍数(1:5000-1:20000不等)由其浓度决定,每孔加100µL,37度反应30分钟后重复清洗步奏4次。
9. 拍干后每孔加入100µL的过氧化物酶(HRP)底物TMB生色. 37度大概15-30分钟每孔加100µL2M硫酸终止反应,然后在酶联测试仪于波长450纳米读数.
10. 结果判断、记录:计算OD为50%时,血清(或抗体)的稀释度,以稀释度为样品的滴度,记录滴度,并将实验结果报告相关人员。
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Bacitracin zinc | 1405-89-6 |
Ammonium Glycyrrhizinate (AMGZ) | 1407-03-0 |
Levosimendan | 141505-33-1 |
Sulfamethizole (Proklar) | 144-82-1 |
Diphenhydramine HCl (Benadryl) | 147-24-0 |
Thiamphenicol (Thiophenicol) | 15318-45-3 |
Dimesna | 16208-51-8 |
Ezetimibe (Zetia) | 163222-33-1 |
Clevudine (Levovir) | 163252-36-6 |
Sitafloxacin hydrate | 163253-35-8,163253-37-0(H2O),127254-12-0(freebase),346607-37-2(HCl) |
Levamisole HCl(Ergamisol) | 16595-80-5 |
Conivaptan HCl (Vaprisol) | 168626-94-6 |
Posaconazole | 171228-49-2,177571-33-4,1198769-38-8(H2O) |
Allopurinol Sodium (Aloprim) | 17795-21-0 |
Cabazitaxel (Jevtana) | 183133-96-2,890654-44-1 |
Telithromycin (Ketek) | 191114-48-4 |
Prazosin HCl | 19237-84-4,19216-56-9(freebase) |
Tiopronin (Thiola) | 1953-02-2 |
Resistomycin | 20004-62-0,1393-09-5 |
Deferasirox (Exjade) | 201530-41-8 |
Flucytosine (Ancobon) | 2022-85-7 |
Oxibendazole | 20559-55-1 |
BIBR 953(Dabigatran etexilate, Pradaxa) | 211914-51-1 |
Tempol (4-Hydroxy-TEMPO) | 2226-96-2 |
Cathepsin Inhibitor 1 | 225120-65-0 |
Aspartame | 22839-47-0 |
Peramivir | 229614-55-5,330600-85-6,1041434-82-5(3H2O) |
Articaine HCl | 23964-57-0,23964-58-1(freebase) |
Econazole nitrate (Spectazole) | 24169-02-6 |
Mycophenolic (Mycophenolate) | 24280-93-1 |
Geniposidic acid | 27741-01-1,103530-48-9(monosodiumsalt) |
Bumetanide | 28395-03-1,28434-74-4(sodiumsalt) |
Ciclopirox (Penlac) | 29342-05-0 |
Ticarcillin sodium | 29457-07-6,34787-01-4(freebase) |
Teniposide (Vumon) | 29767-20-2 |
Methoxsalen (Oxsoralen) | 298-81-7,12692-94-3 |
Gossypol | 303-45-7,40112-23-0,732279-01-5 |
Hydralazine HCl | 304-20-1 |
Calcitriol (Rocaltrol) | 32222-06-3 |
Tobramycin | 32986-56-4 |
Fenoprofen calcium | 34597-40-5 |
Amoxicillin sodium (Amox) | 34642-77-8 |
Buflomedil HCl | 35543-24-9 |
Fluocinonide(Vanos) | 356-12-7 |
Ecdysone | 3604-87-3 |
Prostaglandin E2 (Cervidil) | 363-24-6 |
Doripenem Hydrate | 364622-82-2,148016-81-3(freebase) |
Ribavirin (Copegus) | 36791-04-5 |
Chlorhexidine HCl | 3697-42-5,55-56-1(freebase) |
Azalomycin-B | 37318-06-2,11003-23-9,106387-86-4 |
Betamethasone (Celestone) | 378-44-9,956901-32-9(XHCl) |
Streptomycin sulfate | 3810-74-0 |
Sulindac (Clinoril) | 38194-50-2,63804-15-9(sodiumsalt) |
Nalidixic acid (NegGram) | 389-08-2 |
Suprofen (Profenal) | 40828-46-4 |
Flumequine | 42835-25-6,143984-63-8,42835-68-7(sodiumsalt) |
Fenbendazole (Panacur) | 43210-67-9 |
Azathioprine (Azasan, Imuran) | 446-86-6 |
DL-Carnitine HCl | 461-05-2 |
Lactulose (Enulose) | 4618-18-2,149252-91-5(3H2O) |
Nobiletin (Hexamethoxyflavone) | 478-01-3 |
Rhein(Monorhein) | 478-43-3,202053-60-9(monosodiumsalt) |
Vecuronium Bromide | 50700-72-6,86029-43-8(freebase),74531-76-3(Maleicacid) |
Acipimox | 51037-30-0,76958-97-9(sodiumsalt) |
Flurbiprofen (Ansaid) | 5104-49-4 |
L-NAME HCl | 51298-62-5,50903-99-6(freebase) |
Pizotifen malate | 5189-11-7,15574-96-6(freebase) |
Hesperidin | 520-26-3 |
Rheochrysidin (Physcione) | 521-61-9,140670-52-6 |
Pranoprofen | 52549-17-4 |
Kinetin (6-Furfuryladenine) | 525-79-1 |
Penicillamine (Cuprimine) | 52-67-5 |
Haloperidol (Haldol) | 52-86-8 |
Salinomycin (Procoxacin) | 53003-10-4 |
Prednisone(Adasone) | 53-03-2 |
Mitotane (Lysodren) | 53-19-0,102976-59-0 |
Ethionamide | 536-33-4 |
Oxfendazole | 53716-50-0 |
Indomethacin (Indocid, Indocin) | 53-86-1 |
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