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人血管生成素受体/含免疫球蛋白样环和上皮生长因子样域酪氨酸激酶1(Tie1)ELISA试剂盒
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人血管生成素受体/含免疫球蛋白样环和上皮生长因子样域酪氨酸激酶1(Tie1)elisa试剂盒实验原理
用纯化的抗体包被微孔板,制成固相载体,往包被抗该指标抗体的微孔中依次加入标本或标准品、生物素化的抗该指标抗体、HRP标记的亲和素,经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成*终的黄色。颜色的深浅和样品中的该指标呈正相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样品浓度。
特异性:本试剂盒可同时检测天然或重组的,且与其他相关蛋白无交叉反应。
有效期:6个月
预期应用:ELISA法定量测定血清、血浆、细胞培养上清或其它相关生物液体中的含量。
说明
1.试剂盒保存:-20℃(较长时间不用时);2-8℃(频繁使用时)。
2.浓洗涤液低温保存会有盐析出,稀释时可在水浴中加温助溶。
3.中、英文说明书可能会有不一致之处,请以英文说明书为准。
4.刚开启的酶联板孔中可能会含有少许水样物质,此为正常现象,不会对实验结果造成任何影响。
人血管生成素受体/含免疫球蛋白样环和上皮生长因子样域酪氨酸激酶1(Tie1)ELISA试剂盒组成及试剂配制
1. 酶联板(Assay plate ):一块(96孔)。
2. 标准品(Standard):2瓶(冻干品)。
3. 样品稀释液(Sample Diluent):1×20ml/瓶。
4. 生物素标记抗体稀释液(Biotin-antibody Diluent):1×10ml/瓶。
5. 辣根过氧化物酶标记亲和素稀释液 (HRP-avidin Diluent):1×10ml/瓶。
6. 生物素标记抗体(Biotin-antibody):1×120μl/瓶(1:100)
7. 辣根过氧化物酶标记亲和素(HRP-avidin):1×120μl/瓶(1:100)
8. 底物溶液(TMB Substrate):1×10ml/瓶。
9. 浓洗涤液(Wash Buffer):1×20ml/瓶,使用时每瓶用蒸馏水稀释25倍。
10. 终止液(Stop Solution):1×10ml/瓶(2N H2SO4)。
在收集样本前都必须有一个完整的计划,必须清楚要检测的成份是否足够稳定。对收集后当天就进行
检测的样本,及时储存在4℃备用。对于隔天再检测的样本,及时分装后冻存在-20℃备用,有条件的,-70℃冻存备用。标本应避免反复冻融。
液体类标本: 包括血清、血浆、尿液、胸腹水、脑脊液、细胞培养上清等。
1. 血清:
室温血液自然凝固10-20分钟后,离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。
2. 血浆:
应根据标本的要求选择EDTA、柠檬酸钠或肝素作为抗凝剂,混合10-20分钟后,离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。
3. 尿液:
用无菌管收集。离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。胸腹水、脑脊液参照此实行。
4. 细胞培养上清:
检测分泌性的成份时,用无菌管收集。离心20分钟左右(2000-3000转/分)。仔细收集上清。
5. 培养细胞
检测细胞内的成份时,用PBS(PH7.2-7.4)稀释细胞悬液,细胞浓度达到100万/ml左右。通过反复冻融或加入组织蛋白萃取试剂,以使细胞破坏并放出细胞内成份。离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。
6. 组织标本
切割标本后,称取重量。加入一定量的PBS,PH7.4。用液氮迅速冷冻保存备用。标本融化后仍然保持2-8℃的温度。加入一定量的PBS(PH7.4),或组织蛋白萃取试剂, 用手工或匀浆器将标本匀浆化。离心20分钟左右(2000-3000转/分)。仔细收集上清。分装后一份待检测,其余冷冻备用。
人血管生成素受体/含免疫球蛋白样环和上皮生长因子样域酪氨酸激酶1(Tie1)ELISA试剂盒其他相关产品发布:
Parkin (Ab-131) Antibody | O60260 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 | |
Parkin (Phospho-Ser131) Antibody | O60260 | Human, Mouse | IHC: 1:50~1:100 ELISA: 1:10000 | |
PAK1/2/3 Antibody | Q13153/Q13177/O75914 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:1000 | |
PAK1/2/3 (Ab-423/402/421) Antibody | Q13153/Q13177/O75914 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 | |
CCR7 Antibody | P32248 | Human | WB: 1:500~1:3000 IF: 1:100~1:500 ELISA: 1:40000 | |
PAK1 (Ab-212) Antibody | Q13153 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
p90 RSK (Ab-573) Antibody | Q15418 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
p90 RSK (Ab-380) Antibody | Q15418 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
p90 RSK (Ab-359/363) Antibody | Q15418 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
p90 RSK (Phospho-Thr359+Ser363) Antibody | Q15418 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
p70 S6 Kinase (Ab-418) Antibody | P23443 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 | |
p70 S6 Kinase (Phospho-Ser418) Antibody | P23443 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 | |
p70 S6 Kinase (Ab-389/412) Antibody | P23443 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:1000 | |
p70 S6 Kinase (Ab-371) Antibody | P23443 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 | |
p70 S6 Kinase (Phospho-Ser371) Antibody | P23443 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 | |
p70 S6 Kinase (Ab-229) Antibody | P23443 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000 | |
p70 S6 Kinase (Phospho-Thr229) Antibody | P23443 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
p53 (Ab-392) Antibody | P04637 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000 | |
p53 (Phospho-Ser392) Antibody | P04637 | Human | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
p53 (Ab-20) Antibody | P04637 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 | |
p53 (Phospho-Ser20) Antibody | P04637 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:1000 | |
p300 (Ab-89) Antibody | Q09472 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:1000 | |
p300 (Phospho-Ser89) Antibody | Q09472 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:1000 | |
nNOS (Ab-852) Antibody | P29476 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000 | |
n-NOS (Phospho-Ser852) Antibody | P29476 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
NMDAR2B (Ab-1474) Antibody | Q13224 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:1000 | |
NMDAR2B (Phospho-Tyr1474) Antibody | Q01097 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
NF-κB p65 (Ab-505) Antibody | Q04206 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:40000 | |
NF-κB p65 (Phospho-Thr505) Antibody | Q04206 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 92%, Positives = 100%);Rat (Identities = 92%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:1000 | |
NF-κB p105/p50 Antibody | P19838 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 92%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:1000 | |
NFAT4 (Ab-165) Antibody | Q12968 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:10000 | |
NFAT4 (Phospho-Ser165) Antibody | Q12968 | Human, Mouse | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000 | |
NFAT3 (Ab-676) Antibody | Q14934 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
NFAT3 (Phospho-Ser676) Antibody | Q14934 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:1000 | |
NFAT2 (Phospho-Ser294) Antibody | O95644 | Human, Mouse | IHC: 1:50~1:100 ELISA: 1:1000 | |
MYPT1 (Ab-853) Antibody | O14974 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
MYPT1 (Phospho-Thr853) Antibody | O14974 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
MYPT1 (Ab-696) Antibody | O14974 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
MYPT1 (Phospho-Thr696) Antibody | O14974 | Human, Mouse, Rat | IHC: 1:50~1:100 IF: 1:500~1:1000 ELISA: 1:1000 | |
MYOD (Phospho-Ser200) Antibody | P15172 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:5000 | |
MYB (Ab-12) Antibody | P10242 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 | |
MYB (Phospho-Ser12) Antibody | P10242 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
MUNC-18a (Ab-313) Antibody | P61764 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000 | |
MUNC-18a (Phospho-Ser313) Antibody | P61764 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:1000 | |
MRLC2 Antibody | P19105 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 92%, Positives = 92%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
MITF (Ab-180/73) Antibody | O75030 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 | |
MITF (Phospho-Ser180/73) Antibody | O75030 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:1000 | |
Met (Phospho-Tyr1356) Antibody | Q09YK0 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:10000 | |
MEF2D Antibody | Q14814 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:1000 | |
MEF2D (Phospho-Ser444) Antibody | Q14814 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 | |
MEF2C (Phospho-Ser387) Antibody | Q06413 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 92%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:10000 | |
MDM2 (Ab-166) Antibody | Q00987 | Human, Mouse | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000 | |
MDM2 (Phospho-Ser166) Antibody | Q00987 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
M-CSF Receptor (Ab-809) Antibody | P07333 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:10000 | |
M-CSF Receptor (Phospho-Tyr809) Antibody | P07333 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:1000 | |
Lck (Ab-192) Antibody | P06239 | Human, Mouse | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000 | |
Lck (Phospho-Tyr192) Antibody | P06239 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:10000 | |
Lck (Ab-505) Antibody | P06239 | Human, Mouse | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000 | |
Lck (Phospho-Tyr505) Antibody | P06239 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:60000 | |
LAT (Ab-191) Antibody | O43561 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:5000 | |
LAT (Phospho-Tyr191) Antibody | O43561 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
Lamin A/B (Ab-392) Antibody | P02545 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
Lamin A/C (Phospho-Ser392) Antibody | P02545 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
KIT (Phospho-Tyr703) Antibody | P10721 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
KIT (Ab-936) Antibody | P10721 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:5000 | |
KIT (Phospho-Tyr936) Antibody | P10721 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
JNK1/2/3 (Ab-183/185) Antibody | P45983/P45984/P53779 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000 | |
JNK1/2/3 (Phospho-Thr183+Tyr185) Antibody | P45983/P45984/P53779 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:10000 | |
JAK2 (Ab-570) Antibody | O60674 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000 | |
JAK2 (Phospho-Tyr570) Antibody | O60674 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:1000 | |
IRS-1 Antibody | P35568 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
IRS-1 (Phospho-Ser323) Antibody | P35568 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 | |
IRF3 (Ab-385) Antibody | Q14653 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
IRF-3 (Phospho-Ser385) Antibody | Q14653 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 | |
IRAK1 (Ab-387) Antibody | P51617 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:1000 | |
IRAK1 (Phospho-Thr387) Antibody | P51617 | Human, Mouse, Rat | IF: 1:100~1:500 ELISA: 1:1000 | |
IR (Ab-1361) Antibody | P06213 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:1000 | |
IR (Phospho-Tyr1361) Antibody | P06213 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 | |
IR (Ab-1355) Antibody | P06213 | Human (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
IR (Phospho-Tyr1355) Antibody | P06213 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 | |
IR Antibody | P06213 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IF: 1:100~1:500 ELISA: 1:1000 |
热门标签:人血管生成素受体 Tie1 ELISA 人血管生成素受体试剂盒