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人巨细胞病毒抗体IgG(CMV IgG)ELISA试剂盒*今品化学新闻
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品牌名称：$brandModel.Title(进口品牌)型号：48T/96T 原产地：中国大陆 发布时间：2018/7/26 15:06:19更新时间：2024/10/17 11:31:32
产品摘要：人巨细胞病毒抗体IgG(CMV IgG)ELISA试剂盒*今品化学新闻为今品化学技术（上海）有限公司主打产品，质量好,发货及时,灵敏度高，特异性强，重复性好，其他动物类试剂盒种属一应俱全,指标数千种。公司配有专业实验室可寄样免费代检测支持现场观摩,提供售前售中售后全方位服务，欢迎来电询价或在线咨询索取产品说明书~
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人巨细胞病毒抗体IgG(CMV IgG)elisa试剂盒*今品化学新闻操作步骤
1. 标准品的稀释与加样：在酶标包被板上设标准品孔10孔，在、第二孔中分别加标准品100μl，然后在、第二孔中加标准品稀释液50μl，混匀；然后从孔、第二孔中各取100μl分别加到第三孔和第四孔，再在第三、第四孔分别加标准品稀释液50μl，混匀；然后在第三孔和第四孔中先各取50μl弃掉，再各取50μl分别加到第五、第六孔中，再在第五、第六孔中分别加标准品稀释液50ul，混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中，再在第七、第八孔中分别加标准品稀释液50μl，混匀后从第七、第八孔中分别取50μl加到第九、第十孔中，再在第九第十孔分别加标准品稀释液50μl，混匀后从第九第十孔中各取50μl弃掉。（稀释后各孔加样量都为50μl，浓度分别为9U/L，6U/L，3U/L，1.5U/L，0.75U/L）。
2. 加样：分别设空白孔（空白对照孔不加样品及酶标试剂，其余各步操作相同）、待测样品孔。在酶标包被板上待测样品孔中先加样品稀释液40μl，然后再加待测样品10μl（样品*终稀释度为5倍）。加样将样品加于酶标板孔底部，尽量不触及孔壁，轻轻晃动混匀。
3. 温育：用封板膜封板后置37℃温育30分钟。
4. 配液：将20倍浓缩洗涤液用蒸馏水20倍稀释后备用。
5. 洗涤：小心揭掉封板膜，弃去液体，甩干，每孔加满洗涤液，静置30秒后弃去，如此重复5次，拍干。
6. 加酶：每孔加入酶标试剂50μl，空白孔除外。
7. 温育：操作同3。
8. 洗涤：操作同5。
9. 显色：每孔先加入显色剂A50μl，再加入显色剂B50μl，轻轻震荡混匀，37℃避光显色15分钟.
10. 终止：每孔加终止液50μl，终止反应（此时蓝色立转黄色）。
11. 测定：以空白空调零，450nm波长依序测量各孔的吸光度（OD值）。测定应在加终止液后15分钟以内进行
每个试剂盒操作是不同的，请按说明书操作。具体事项请来电咨询！
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| 人巨细胞病毒抗体(CMV)ELISA试剂盒 |
| CD1E Antibody | P15812 | Human (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:10000 | |
| CD6 Antibody | P30203 | Human (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
| TAGAP Antibody | Q8N103 | Human (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:1000 | |
| TBX18 Antibody | O95935 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:20000 | |
| SDC2 Antibody | P34741 | Human (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:1000 | |
| SYTL4 Antibody | Q96C24 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
| SYT16 Antibody | Q17RD7 | Human (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
| SYT13 Antibody | Q7L8C5 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%) Rat (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:10000 | |
| SYT11 Antibody | Q9BT88 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%) Rat (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:10000 | |
| SVOP Antibody | Q8N4V2 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
| UBASH3A Antibody | P57075 | Human (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:1000 | |
| ZNF280C Antibody | Q8ND82 | Human (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
| ZNF280A Antibody | P59817 | Human (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
| STK39 Antibody | Q9UEW8 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
| STMN4 Antibody | Q9H169 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
| STMN3 Antibody | Q9NZ72 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:10000 | |
| SFRS8 Antibody | Q12872 | Human (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:20000 | |
| SFRS7 Antibody | Q16629 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
| SFRS2B Antibody | Q9BRL6 | Human (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
| SFRS19 Antibody | Q9H7N4 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 92%, Positives = 92%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
| SFRS15 Antibody | O95104 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 93%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
| SFRS11 Antibody | Q05519 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:10000 | |
| SF3B4 Antibody | Q15427 | Human (Identities = 100%, Positives = 100%)Mouse (Identities = 100%, Positives = 100%) Rat (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000 | |
| SF3B3 Antibody | Q15393 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%) | WB: 1:500~1:3000 ELISA: 1:10000 | |
| SPTBN5 Antibody | Q9NRC6 | Human (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:10000 | |
| SPTB2 Antibody | Q01082 | Human | WB: 1:500~1:1000 ELISA: 1:10000 | |
| SNX4 Antibody | O95219 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 92%, Positives = 100%); Rat (Identities = 92%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:10000 | |
| SNX3 Antibody | O60493 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:5000 | |
| SNX1 Antibody | Q13596 | Human; Mouse; Rat | WB: 1:500~1:3000 ELISA: 1:40000 | |
| SOS2 Antibody | Q07890 | Human (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:10000 | |
| SLCO1B1 Antibody | Q9Y6L6 | Human (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
| SLCO1A2 Antibody | O60493 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:5000 | |
| SLC4A8/10 Antibody | Q6U841/Q2Y0W8 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
| SLC17A2 Antibody | O00624 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
| SLC5A6 Antibody | Q9Y289 | Human | WB: 1:500~1:1000 ELISA: 1:10000 | |
| SLC6A3 Antibody | Q01959 | Human; Mouse; Rat | WB: 1:500~1:3000 ELISA: 1:10000 | |
| ATP1B3 Antibody | P54709 | Human | WB: 1:500~1:3000 ELISA: 1:10000 | |
| ATP1A2 Antibody | P50993 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:1000 | |
| SLC24A6 Antibody | Q8NFF2 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 94%, Positives = 100%);Rat (Identities = 94%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
| SLC24A4 Antibody | Q8NFF2 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 94%, Positives = 100%);Rat (Identities = 94%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
| SLC24A3 Antibody | Q9HC58 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:20000 | |
| SLC24A1 Antibody | O60721 | Human (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:10000 | |
| SLC28A2 Antibody | O43868 | Human (Identities = 100%, Positives = 100%);Rat (Identities = 86%, Positives = 100%) | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 | |
| SLC5A3 Antibody | P53794 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 93%, Positives = 100%); Rat (Identities = 93%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:10000 | |
| SLC9A9 Antibody | Q8IVB4 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 91%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
| SLC9A8 Antibody | Q9Y2E8 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 84%, Positives = 92%);Rat (Identities = 84%, Positives = 92%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
| SLC9A7 Antibody | Q96T83 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:10000 | |
| SLC9A6 Antibody | Q92581 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
| SLC5A2 Antibody | P31639 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 91%, Positives = 91%);Rat (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
| SLC10A7 Antibody | Q0GE19 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
| SCN2B Antibody | O60939 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 93%, Positives = 93%); Rat (Identities = 93%, Positives = 93%) | WB: 1:500~1:1000 ELISA: 1:20000 | |
| SCN9A Antibody | Q15858 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 92%, Positives = 100%);Rat (Identities = 92%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:40000 | |
| SCN7A Antibody | Q01118 | Human (Identities = 100%, Positives = 100%) | IF: 1:100~1:500 ELISA: 1:20000 | |
| SCN4A Antibody | P35499 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:10000 | |
| SCN4B Antibody | P16949 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:10000 | |
| SLC4A11 Antibody | Q8NBS3 | Human | WB: 1:500~1:1000 ELISA: 1:20000 | |
| SLC6A6 Antibody | P31641 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:10000 | |
| SLC6A1 Antibody | P30531 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:40000 | |
| SLC6A8 Antibody | P48029 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:10000 | |
| SIRPG Antibody | Q9P1W8 | Human | WB: 1:500~1:3000 ELISA: 1:10000 | |
| SIRPB1 Antibody | O00241 | Human (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:1000 | |
| SGOL2 Antibody | Q562F6 | Human (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:10000 | |
| SHC3 Antibody | P16949 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 91%, Positives = 91%);Rat (Identities = 91%, Positives = 91%) | IHC: 1:50~1:100 ELISA: 1:10000 | |
| SHC2 Antibody | P98077 | Human (Identities = 100%, Positives = 100%);Rat (Identities = 84%, Positives = 84%) | WB: 1:500~1:1000 ELISA: 1:10000 | |
| SH3GL2 Antibody | Q99962 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:10000 | |
| SH3BGR Antibody | P55822 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 91%, Positives = 100%); Rat (Identities = 91%, Positives = 100%) | WB: 1:500~1:1000 ELISA: 1:10000 | |
| SH3GLB2 Antibody | Q9NR46 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000 |
热门标签：人巨细胞病毒抗体IgG CMV IgG ELISA 人巨细胞病毒抗体IgG试剂盒
