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人淋球菌抗体快速检测(NG-Ab)ELISA试剂盒*新闻快讯
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人淋球菌抗体快速检测(NG-Ab)elisa试剂盒*新闻快讯操作步骤:
1.编号:将样品对应微孔按序编号,每板应设阴性对照2孔、阳性对照2孔、空白对照1孔(空白对照孔不加样品及酶标试剂,其余各步操作相同)
2.加样:分别在阴、阳性对照孔中加入阴性对照、阳性对照50μl。然后在待测样品孔先加样品稀释液40μl,然后再加待测样品10μl。加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀,
3.温育:用封板膜封板后置37℃温育30分钟。
4.配液:将30(48T的20倍)倍浓缩洗涤液加蒸馏水至600ml后备用
5.洗涤:小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干。
6.加酶:每孔加入酶标试剂50μl,空白孔除外。
7.温育:操作同3。
8.洗涤:操作同5。
9.显色:每孔先加入显色剂A 50μl,再加入显色剂B 50μl,轻轻震荡混匀,37℃避光显色15分钟
10.终止:每孔加终止液50μl,终止反应(此时蓝色立转黄色)。
11.测定:以空白空调零,450nm波长依序测量各孔的吸光度(OD值)。 测定应在加终止液后15分钟以内进行。
人淋球菌抗体快速检测(NG-Ab)ELISA试剂盒*新闻快讯注意事项
1.操作严格按照说明书进行,本试剂不同批号组分不得混用。
2.试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存。
3.浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果。
4.封板膜只限一次性使用,以避免交叉污染。
5.底物请避光保存。
6.试验结果判定必须以酶标仪读数为准,使用双波长检测时,参考波长为630nm
7.所有样品,洗涤液和各种废弃物都应按传染物处理。终止液为2M的硫酸,使用时必须注意安全。
人淋球菌抗体快速检测(NG-Ab)ELISA试剂盒*新闻快讯相关产品发布:
10-024-CVR | 500 mL Improved MEM (Richter's Mod.) with L-glutamine | IMEM培养基 (含谷氨酰胺) | Media Standard |
10-026-CVR | 500 mL Improved MEM (Richter's Mod.) with L-glutamine, without phenol red | IMEM培养基 (含谷氨酰胺,不含酚红) | Media Standard |
10-027-CVR | DMEM (Dulbecco's Modification of Eagle's Medium) with 25 mM HEPES, 4.5 g/L glucose, L-glutamine, without sodium pyruvate | DMEM培养基(HEPES,含谷氨酸钠,不含丙酮酸钠) | Media Standard |
10-040-CMR | RPMI 1640 with L-glutamine,1L | RPMI1640培养基 (含谷氨酰胺) | Media Standard |
10-040-LBR | 20 L RPMI 1640 with L-glutamine | RPMI1640培养基 (含谷氨酰胺) | Media Standard |
10-040-LXR | 10 L RPMI 1640 with L-glutamine | RPMI1640培养基 (含谷氨酰胺) | Media Standard |
10-041-CMR | 1 L RPMI 1640 with L-glutamine and 25 mM HEPES | RPMI1640培养基 (含谷氨酰胺,HEPES) | Media Standard |
10-043-CVR | 500 mL RPMI 1640 with L-glutamine, without glucose | RPMI1640培养基 (含谷氨酰胺,无糖) | Media Standard |
10-050-CVR | 500 mL McCoy's 5A (Iwakata & Grace Modification) with L-glutamine | 麦考伊5A(含谷氨酰胺) | Media Standard |
10-051-CIR | 100 mL McCoy's 5A (Iwakata & Grace Modification) with L-glutamine and 25 mM HEPES. | 麦考伊5A(含谷氨酰胺,HEPES) | Media Standard |
10-080-CMR | 1 L Ham's F-12 Medium with L-glutamine | Ham's F12 培养基 (含谷氨酰胺) | Media Standard |
10-090-CMR | 1 L DMEM (Dulbecco's Modification of Eagle's Medium)/Ham's F-12 50/50 Mix with L-glutamine | DMEM/F12培养基 (含谷酰胺) | Media Standard |
10-090-CVR | 500 mL DMEM (Dulbecco's Modification of Eagle's Medium)/Ham's F-12 50/50 Mix with L-glutamine | DMEM/F12培养基 (含谷酰胺) | Media Standard |
10-092-CMR | 1 L DMEM (Dulbecco's Modification of Eagle's Medium)/Ham's F-12 50/50 Mix with L-glutamine and 15 mM HEPES | DMEM/F12培养基 (含谷氨酰胺, HEPES) | Media Standard |
10-101-CVR | 500 mL DMEM (Dulbecco's Modification of Eagle's Medium), 1x with glutagro Supplement, 4.5 g/L glucose, sodium pyruvate and Phenol Red | DMEM培养基(含丙酮酸钠和苯酚红) | Media Standard |
10-102-CVR | 500 mL DMEM (Dulbecco's Modification of Eagle's Medium) with glutagrO, 4.5 g/L glucose, phenol red, without sodium pyruvate | DMEM培养基(含丙酮酸钠,不含苯酚红) | Media Standard |
10-103-CVR | 500 mL DMEM (Dulbecco's Modification of Eagle's Medium)/Ham's F-12 50/50 Mix with glutagro Supplement | DMEM/F12培养基 (含谷氨酰胺) | Media Standard |
10-104-CVR | 500 mL RPMI 1640, 1x with glutagro supplement and Phenol Red | RPMI1640培养基(含谷氨酰胺) | Media Standard |
13-100-CV | 500 mL Hink's TNM-FH Medium with L-glutamine lactalbumin hydrolysate, and yeastolate, without insect hemolymph | Hink's TNM-FH 培养基(不含昆虫淋巴液) | Specialty Products |
13-402-CV | 500 mL Insectagro DS2 Serum-Free/Protein-Free Medium, 1x without L-glutamine | Insectagro DS2 无血清/无蛋白培养基 1x 不含谷氨酸钠 | Specialty Products |
13-410-CV | 500 mL Insectagro Sf9 Serum-Free/Protein-Free Medium, with L-glutamine | Insectagro Sf9 无血清/无蛋白培养基,含谷氨酸钠 | Specialty Products |
13-901-CI | 100 mL Poloxamer 188 | 泊咯沙姆188 | Specialty Products |
15-010-CMR | 1 L MEM (Minimum Essential Medium) with Earle's salts, without L-glutamine | EME培养基(含Earle的盐,不含谷氨酰胺) | Media Standard |
15-010-CVR | 500 mL MEM (Minimum Essential Medium) with Earle's salts, without L-glutamine | EME培养基(含Earle的盐,不含谷氨酰胺) | Media Standard |
15-012-CVR | 500 mL MEM (Minimum Essential Medium) Alpha Medium with Earle's salts without ribonucleosides, deoxyribonucleosides, and L-glutamine | EME培养基(含Earle's盐无核糖、脱氧核糖核苷,不含谷氨酰胺 | Media Standard |
15-013-CMR | 1 L DMEM (Dulbecco's Modification of Eagles Medium) with 4.5 g/L glucose, sodium pyruvate, without L-glutamine | DMEM培养基 (高糖, 含丙酮酸钠,不含谷氨酰胺) | Media Standard |
15-013-LBR | 20 L DMEM (Dulbecco's Modification of Eagles Medium) with 4.5 g/L glucose, sodium pyruvate, without L-glutamine | DMEM培养基 (高糖, 含丙酮酸钠,不含谷氨酰胺) | Media Standard |
15-013-LXR | 10 L DMEM (Dulbecco's Modification of Eagles Medium) with 4.5 g/L glucose, sodium pyruvate, without L-glutamine | DMEM培养基 (高糖, 含丙酮酸钠,不含谷氨酰胺) | Media Standard |
15-015-CVR | 500 mL MEM (Minimum Essential Medium) (For Suspension Cultures) without L-glutamine, calcium, and magnesium | EME培养基(不含谷氨酰胺,钙,镁) | Media Standard |
15-016-CVR | Iscove's Modification of DMEM with 25 mM HEPES, without -thioglycerol, -mercaptoethanol, and L-glutamine | DMEM培养基 (含HEPES,不含谷氨酰胺) | Media Standard |
15-017-CMR | 1 L DMEM (Dulbeco's Modification of Eagle's Medium) with 4.5 g/L glucose, without L-glutamine, sodium pyruvate | DMEM培养基 (高糖, 不含谷氨酰胺,丙酮酸钠) | Media Standard |
15-018-CMR | 1 L DMEM (Dulbecco's Modification of Eagle's Medium) with 4.5 g/L glucose, 25 mM HEPES, sodium pyruvate, without L-glutamine | DMEM培养基 (含HEPES,丙酮酸钠,不含谷氨酰胺) | Media Standard |
15-018-CVR | 500 mL DMEM (Dulbecco's Modification of Eagle's Medium) with 4.5 g/L glucose, 25 mM HEPES, sodium pyruvate, without L-glutamine | DMEM培养基 (含HEPES,丙酮酸钠,不含谷氨酰胺) | Media Standard |
15-040-CMR | 1 L RPMI 1640 1x, without L-glutamine | RPMI1640培养基 (不含谷氨酰胺) | Media Standard |
15-040-LBR | 20 L RPMI 1640 1x, without L-glutamine | RPMI1640培养基 (不含谷氨酰胺) | Media Standard |
15-090-CMR | 1 L DMEM (Dulbecco's Modification of Eagle's Medium)/F12 50-50 Mix without L-glutamine | DMEM/F12培养基 (不含谷氨酰胺) | Media Standard |
15-100-CVR | 500 mL MCDB 131, 1x | MCDB 131(含谷氨酰胺) | Media Standard |
17-104-CIR | 100 mL RPMI 1640 without L-glutamine, L-methionine, and L-cystine | RPMI 1640(不含谷氨酰胺) | Media Standard |
17-105-CVR | 500 mL RPMI 1640 without L-glutamine and phenol red | RPMI 1640(不含谷氨酰胺,酚红) | Media Standard |
17-204-CIR | 100 mL DMEM (Dulbecco's Modification of Eagle's Medium) with 4.5 g/L glucose and sodium pyruvate without L-glutamine, L-methionine, and L-cystine | DMEM培养基 (不含谷氨酰胺,含丙酮酸钠) | Media Standard |
17-205-CVR | 500 mL DMEM (Dulbecco's Modification of Eagle's Medium) with 4.5 g/L glucose and sodium pyruvate without L-glutamine and phenol red | DMEM培养基 (不含谷氨酰胺,酚红,含丙酮酸钠) | Media Standard |
17-206-CIR | 100 mL DMEM (Dulbecco's Modification of Eagle's Medium) with 4.5 g/L glucose and sodium pyruvate without L-glutamine, and phosphate | DMEM培养基 (含丙酮酸钠,不含谷氨酰胺,磷酸盐) | Media Standard |
17-207-CVR | 500 mL DMEM (Dulbecco's Modification of Eagle's Medium), without glucose, L-glutamine, and sodium pyruvate | DMEM培养基 (不含谷氨酰胺,丙酮酸钠) | Media Standard |