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人莱姆病IgG,IgM(Lyme IgG IgM)ELISA试剂盒*新闻快讯
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人莱姆病IgG,IgM(Lyme IgG IgM)elisa试剂盒*新闻快讯操作步骤:
1.编号:将样品对应微孔按序编号,每板应设阴性对照2孔、阳性对照2孔、空白对照1孔(空白对照孔不加样品及酶标试剂,其余各步操作相同)
2.加样:分别在阴、阳性对照孔中加入阴性对照、阳性对照50μl。然后在待测样品孔先加样品稀释液50μl,然后再加待测样品10μl。加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀,
3.温育:用封板膜封板后置37℃温育30分钟。
4.配液:将30(48T的20倍)倍浓缩洗涤液加蒸馏水至600ml后备用
5.洗涤:小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干。
6.加酶:每孔加入酶标试剂50μl,空白孔除外。
7.温育:操作同3。
8.洗涤:操作同5。
9.显色:每孔先加入显色剂A 50μl,再加入显色剂B 50μl,轻轻震荡混匀,37℃避光显色15分钟
10.终止:每孔加终止液50μl,终止反应(此时蓝色立转黄色)。
11.测定:以空白空调零,450nm波长依序测量各孔的吸光度(OD值)。 测定应在加终止液后15分钟以内进行。
人莱姆病IgG,IgM(Lyme IgG IgM)ELISA试剂盒*新闻快讯注意事项
1.操作严格按照说明书进行,本试剂不同批号组分不得混用。
2.试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存。
3.浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果。
4.封板膜只限一次性使用,以避免交叉污染。
5.底物请避光保存。
6.试验结果判定必须以酶标仪读数为准,使用双波长检测时,参考波长为630nm
7.所有样品,洗涤液和各种废弃物都应按传染物处理。终止液为2M的硫酸,使用时必须注意安全。
人莱姆病IgG,IgM(Lyme IgG IgM)ELISA试剂盒*新闻快讯相关产品发布:
Dulbecco's Modification of Eagle's Medium/Ham's F-12 50/50 Mix, Powder without sodium bicarbonate and L-glutamine,1X50L | DMEM/F12培养基 (含丙酮酸钠,不含谷氨酰胺和碳酸氢钠) | Media Standard |
Collection Container 25L, Metallocene Film | 25L收集袋(储液袋) | Media Custom |
Collection Container 10L, Metallocene Film | 10L收集袋(储液袋) | Media Custom |
Collection Container 30L, Metallocene Film | 50L收集袋(储液袋) | Media Custom |
Collection Container 100L, Metallocene Film | 100L收集袋(储液袋) | Media Custom |
Collection Container 1L, EVA Film | 1L收集袋(储液袋) | Media Custom |
Collection Container 2L, EVA Film | 2L收集袋(储液袋) | Media Custom |
Collection Container 5L, EVA Film | 5L收集袋(储液袋) | Media Custom |
Collection Container 10L, EVA Film | 10L收集袋(储液袋) | Media Custom |
Collection Container 20L, EVA Film | 20L收集袋(储液袋) | Media Custom |
Collection Container 10L, EVA Film | 10L收集袋(储液袋) | Media Custom |
Collection Container 20L, EVA Film | 20L收集袋(储液袋) | Media Custom |
Collection Container 500 mL, EVA Film | 500ml储液袋 | Media Custom |
Collection Container 1L, EVA Film | 1L储液袋 | Media Custom |
Collection Container 5L, EVA Film | 5L储液袋 | Media Custom |
Collection Container 10L, EVA Film | 10L储液袋 | Media Custom |
Collection Container 20L, EVA Film | 20L储液袋 | Media Custom |
Collection Container 50L, EVA Film | 50L储液袋 | Media Custom |
50L Gusseted Bag w/PE film | 50L角撑袋 PE材质 | Media Custom |
Single-use Trypsin Bag for HYPERStack vessel, 5L | 胰酶袋 | Media Custom |
Single-use Quench bag for HYPERStack vessel, 5L | 缓冲液袋 | Media Custom |
Single-use Media bag for HYPERStack vessel, 20L | 培养基袋 | Media Custom |
Rocker Cell Culture Bag, 20L | 20L 摇摆式细胞培养袋 | Media Custom |
Rocker Cell Culture Bag, 10L | 10L 摇摆式细胞培养袋 | Media Custom |
Rocker Cell Culture Bag, 2L | 2L 摇摆式细胞培养袋 | Media Custom |
Rocker Cell Culture Bag, 50L | 50L 摇摆式细胞培养袋 | Media Custom |
Collection Container 100L, EVA Film | 100L储液袋 | Media Custom |
Collection Container 200L, EVA Film | 200L储液袋 | Media Custom |
Cell Expansion Bag, 500mL | 500ML 细胞扩增袋 | Media Custom |
Cell Expansion Bag, 1L | 1L 细胞扩增袋 | Media Custom |
Cell Expansion Bag, 3L | 3L 细胞扩增袋 | Media Custom |
Cell Expansion Bag, 5L | 5L 细胞扩增袋 | Media Custom |
Cryopreservation Bag, 50mL | 10-20ml 冻存袋 | Media Custom |
Cryopreservation Bag, 250mL | 30-70ml 冻存袋 | Media Custom |
Cryopreservation Bag, 500mL | 55-100ml 冻存袋 | Media Custom |
Cryopreservation Bag, 750mL | 80-190ml 冻存袋 | Media Custom |
Tank Liner 50L, Gusseted, 3D | 50L 角撑衬套 | Media Custom |
Tank Liner 130L | 130L 衬套 | Media Custom |
Tank Liner 100L, Gusseted, 3D | 100L 角撑衬套 | Media Custom |
Tank Liner 200L | 200L 衬套 | Media Custom |
Tank Liner 200L, Gusseted, 3D | 200L 角撑衬套 | Media Custom |
560 L 2D Tank Liner | 560L衬套 | Media Custom |
Tank Liner 1090L | 1090L 衬套 | Media Custom |
C-FLEX tubing, 3- 1/4" ID, with Male Luer with cap to Female Luer | 管套 C-Flex 3'x1/4" ID | Media Custom |
TUBE SET C-Flex 3'x1/4" ID | 管套 C-Flex 3'x1/4" ID | Media Custom |
C-FLEX tubing, 3- 1/4" ID, with Female MPC connector to Female Luer w/cap | 管套 C-Flex 3'x1/4" ID | Media Custom |
TUBE SET C-Flex 3'x3/8" ID w/1 | 管套 C-Flex 3'x3/8" ID w/1 | Media Custom |
TUBE SET C-Flex 3'x3/8" ID w/M | 管套 C-Flex 3'x3/8" ID w/M | Media Custom |
C-FLEX tubing, 2-1/4" ID, with Female MPC Connector with 1/4" Barb with Pinch Clamp and Filling Bell | 灌装钟 | Media Custom |
500 mL MEM (Minimum Essential Medium) with 1.5 g/L sodium bicarbonate, NEAA, L-glutamine, and sodium pyruvate | MEM,含非必需氨基酸 | Media Standard |
1 L MEM (Minimum Essential Medium) with Earle's salts and L-glutamine | MEM培养基 (含谷氨酰胺) | Media Standard |
1 L DMEM (Dulbecco's Modification of Eagle's Medium) 4.5 g/L glucose, L-glutamine, and sodium pyruvate | DMEM培养基 (高糖, 含谷氨酰胺,丙酮酸钠) | Media Standard |
20 L DMEM (Dulbecco's Modification of Eagle's Medium) 4.5 g/L glucose, L-glutamine, and sodium pyruvate | DMEM培养基 (高糖, 含谷氨酰胺,丙酮酸钠) | Media Standard |
10 L DMEM (Dulbecco's Modification of Eagle's Medium) 4.5 g/L glucose, L-glutamine, and sodium pyruvate | DMEM培养基 (高糖, 含谷氨酰胺,丙酮酸钠) | Media Standard |
1 L DMEM (Dulbecco's Modification of Eagle's Medium) with 1 g/L glucose, L-glutamine, and sodium pyruvate | DMEM培养基 (低糖, 含谷氨酰胺,丙酮酸钠) | Media Standard |
1 L Iscove's Modification of DMEM with L-glutamine and 25 mM HEPES without ß-thioglycerol and ß-mercaptoethanol | IMDM培养基 (含谷氨酰胺, HEPES,无α-甘油、β-巯基乙) | Media Standard |
Dulbecco's Modification of Eagles Medium (DMEM) with 4.5 g/L | DMEM培养基 (高糖, 不含丙酮酸钠) | Media Standard |