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人戊肝抗体IgG(HEV IgG)ELISA试剂盒*新闻快讯
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产品名称:人戊肝抗体IgG(HEV IgG)elisa试剂盒*新闻快讯
英文名称:HEV IgG ELISA Kit
检测范围:来电咨询(或者发邮件至kmsbio@sina.cn)索取
检测目标:人/动物
样品形式:血清/血浆/细胞培养上清/其它生物液体
保存与运输:短期2-8℃/长期-20℃保存
应用范围:仅用于科研,不得用于医学诊断
人戊肝抗体IgG(HEV IgG)ELISA试剂盒*新闻快讯样本处理及要求:
1. 血清:室温血液自然凝固10-20分钟,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如出现沉淀,应再次离心。
2. 血浆:应根据标本的要求选择EDTA或柠檬酸钠作为抗凝剂,混合10-20分钟后,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如有沉淀形成,应该再次离心。
3. 尿液:用无菌管收集,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如有沉淀形成,应再次离心。胸腹水、脑脊液参照实行。
4. 细胞培养上清:检测分泌性的成份时,用无菌管收集。离心20分钟左右(2000-3000转/分)。仔细收集上清。检测细胞内的成份时,用PBS(PH7.2-7.4)稀释细胞悬液,细胞浓度达到100万/ml左右。通过反复冻融,以使细胞破坏并放出细胞内成份。离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。
5. 组织标本:切割标本后,称取重量。加入一定量的PBS,PH7.4。用液氮迅速冷冻保存备用。标本融化后仍然保持2-8℃的温度。加入一定量的PBS(PH7.4),用手工或匀浆器将标本匀浆充分。离心20分钟左右(2000-3000转/分)。仔细收集上清。分装后一份待检测,其余冷冻备用。
6. 标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验。若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融.
7. 不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的(HRP)活性。
人戊肝抗体IgG(HEV IgG)ELISA试剂盒*新闻快讯注意事项:
· 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存。
· 浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果。
· 各步加样均应使用加样器,并经常校对其准确性,以避免试验误差。一次加样时间控制在5分钟内,如标本数量多,推荐使用排枪加样。
· 请每次测定的同时做标准曲线,做复孔。如标本中待测物质含量过高(样本OD值大于标准品孔孔的OD值),请先用样品稀释液稀释一定倍数(n倍)后再测定,计算时请*后乘以总稀释倍数(×n×5)。
· 封板膜只限一次性使用,以避免交叉污染。
· 底物请避光保存。
· 严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.
· 所有样品,洗涤液和各种废弃物都应按传染物处理。
· 本试剂不同批号组分不得混用。
10. 如与英文说明书有异,以英文说明书为准。
计算:
以标准物的浓度为横坐标,OD值为纵坐标,在坐标纸上绘出标准曲线,根据样品的OD值由标准曲线查出相应的浓度;再乘以稀释倍数;或用标准物的浓度与OD值计算出标准曲线的直线回归方程式,将样品的OD值代入方程式,计算出样品浓度,再乘以稀释倍数,即为样品的实际浓度。
保存条件及有效期:
1.试剂盒保存:;2-8℃。
2.有效期:6个月
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500 mL MEM (Minimum Essential Medium) with Earle's salts, without L-glutamine and phenol red | MEM培养基 (不含谷氨酰胺,酚红) | Media Standard |
500 mL HBSS (Hank's Balanced Salt Solution), 10x without sodium bicarbonate, calcium and magnesium | HBSS平衡盐溶液 (不含碳酸氢铵、钙和镁) | Media Standard |
500 mL HBSS (Hank's Balanced Salt Solution), 10x with calcium and magnesium without phenol red and sodium bicarbonate | HBSS平衡盐溶液 (含钙和镁、不含碳酸氢铵) | Media Standard |
500 mL DPBS (Dulbecco's Phosphate-Buffered Saline), 10x with calcium and magnesium | DPBS缓冲液 (含钙, 镁) | Media Standard |
500 mL DPBS (Dulbecco's Phosphate-Buffered Saline), 10x without calcium and magnesium | DPBS缓冲液 (不含钙, 镁) | Media Standard |
1 L HBSS (Hank's Balanced Salt Solution), 1x with calcium and magnesium | HBSS平衡盐溶液 (含钙, 镁) | Media Standard |
1 L HBSS (Hank's Balanced Salt Solution), 1x without calcium and magnesium | HBSS平衡盐溶液 (不含钙, 镁) | Media Standard |
1 L HBSS (Hank's Balanced Salt Solution), 1x without calcium, magnesium and phenol red | HBSS平衡盐溶液 (不含钙, 镁, 酚红) | Media Standard |
1 L HBSS (Hank's Balanced Salt Solution), 1x with calcium and magnesium without phenol red | HBSS平衡盐溶液 (含钙, 镁, 不含酚红) | Media Standard |
500 mL HBSS (Hank's Balanced Salt Solution), 1x with calcium and magnesium without phenol red | HBSS平衡盐溶液 (含钙, 镁, 不含酚红) | Media Standard |
1 L DPBS (Dulbecco's Phosphate-Buffered Saline), 1x with calcium and magnesium | DPBS缓冲液 (含钙, 镁) | Media Standard |
500 mL DPBS (Dulbecco's Phosphate-Buffered Saline), 1x with calcium and magnesium | DPBS缓冲液 (含钙, 镁) | Media Standard |
1 L DPBS (Dulbecco's Phosphate-Buffered Saline), 1x without calcium and magnesium | DPBS缓冲液 (不含钙, 镁) | Media Standard |
20 L DPBS (Dulbecco's Phosphate-Buffered Saline), 1x without calcium and magnesium | DPBS缓冲液 (不含钙, 镁) | Media Standard |
10 L DPBS (Dulbecco's Phosphate-Buffered Saline), 1x without calcium and magnesium | DPBS缓冲液 (不含钙, 镁) | Media Standard |
1 L PBS (Phosphate-Buffered Saline), 1x without calcium and magnesium | PBS缓冲液 (不含钙, 镁) | Media Standard |
1 L PBS (Phosphate-Buffered Saline), 1x without calcium and magnesium | PBS), 1x 不含钙镁 | Media Standard |
500 mL L-Glutamine 200mM solution | 200mM谷氨酸钠溶液 | Media Standard |
100 mL MEM Vitamins 100x Solution | MEM维生素(100X) | Media Standard |
100 mL Trace Elements A, 1000x | 微量元素 A, 1000x | Media Standard |
100 mL Trace Elements B, 1000x | 微量元素 B, 1000x | Media Standard |
100 mL Trace Elements C, 1000x | 微量元素 C, 1000x | Media Standard |
100 mL MEM Amino Acids, 50x Solution without L-glutamine | MEM氨基酸(不含谷氨酰胺) | Media Standard |
100 mL Sodium Bicarbonate, 7.5% solution | 7.5%碳酸氢钠溶液 | Media Standard |
100 mL HAT (Hypoxanthine, Aminopterin, Thymidine) | HAT (次黄嘌呤、氨喋呤、胸苷) | Media Standard |
100 mL HT (Hypoxanthine, Thymidine) | HT (次黄嘌呤、胸腺嘧啶核苷) | Media Standard |
100 mL Trypsin 1x 0.25% Trypsin in HBSS without calcium and magnesium, Porcine Parvovirus tested | 胰酶 1x 0.25% 胰酶( HBSS,不含钙镁) | Media Standard |
100 mL Trypsin EDTA 1x 0.05% Trypsin/0.53 mM EDTA in HBSS without calcium and magnesium, Porcine Parvovirus tested | 1x 0.05% 胰酶(0.53 mM EDTA in HBSS ,不含钙镁) | Media Standard |
500 mL Trypsin EDTA 1x 0.05% Trypsin/0.53 mM EDTA in HBSS without calcium and magnesium, Porcine Parvovirus tested | 1x 0.05% 胰酶(0.53 mM EDTA in HBSS ,不含钙镁) | Media Standard |
100 mL Trypsin 10x 2.5% Trypsin in HBSS without calcium, magnesium, and phenol red, Porcine Parvovirus tested | 10x 2.5%胰酶( HBSS,含钙镁,酚红) | Media Standard |
100mL, Cell Culture Grade Water; Tested to USP Sterile Water for Injection Specifications | 细胞培养级水,USP级别注射用级水 | Media Standard |
500mL, Cell Culture Grade Water; Tested to USP Sterile Water for Injection Specifications | 细胞培养级水,USP级别注射用级水 | Media Standard |
20L, Cell Culture Grade Water; Tested to USP Sterile Water for Injection Specifications | 细胞培养级水,USP级别注射用级水 | Media Standard |
100L, Cell Culture Grade Water; Tested to USP Sterile Water for Injection Specifications | 细胞培养级水,USP级别注射用级水 | Media Standard |
200L,Cell Culture Grade Water; Tested to USP Sterile Water for Injection Specifications | 细胞培养级水,USP级别注射用级水 | Media Standard |
20L, Cell Culture Grade Water; Tested to USP and EP Sterile Water for Injection Specifications | 细胞培养用水USP、EP灭菌注射用水 | Media Standard |
100L, Cell Culture Grade Water; Tested to USP and EP Sterile Water for Injection Specifications | 细胞培养用水USP、EP灭菌注射用水 | Media Standard |
200L, Cell Culture Grade Water; Tested to USP and EP Sterile Water for Injection Specifications | 细胞培养用水USP、EP灭菌注射用水 | Media Standard |
100 mL Trypan Blue Solution (w/v) in PBS 0.4% (w/v) in normal saline (8.1 g/L NaCl with 0.6 g/L K2HPO4) | 台盼蓝溶液0.4% (w/v) (PBS) | Media Standard |
Corning® 250 mL DMSO (Dimethyl Sulfoxide) | DMSO溶液 | Media Standard |
100 mL Penicillin-Streptomycin Solution, 50x, 5,000 I.U. Penicillin (per mL) 5,000 µg/mL Streptomycin | 50x 青链霉素溶液 | Media Standard |
Penicillin-Streptomycin Solution, 100X 10,000 I.U. Penicillin 10,000 µg/mL Streptomycin | 100x 青链霉素溶液 | Media Standard |
50 mL Amphotericin B, Liquid 250 µg/mL solubilized | 两性霉素 B,(液体) | Media Standard |
Antibiotic-Antimycotic Solution 10,000 I.U. Penicillin (per mL) 10,000 µg/mL Streptomycin 25 µg/mL Amphotericin with 8.5 g/L NaCI | 抗生素和抗真菌剂 10,000 I.U.青霉素(per mL) 10,000 µg/mL链霉素25 µg/mL 两性霉素 with 8.5 g/L NaCI | Media Standard |
10 mL Gentamicin Sulfate, Liquid 50 mg/mL Solution | 硫酸庆大霉素( 50 mg/mL) | Media Standard |
50 mL Kanamycin Sulfate, Liquid, 5,000 µg/mL Solution | 卡那霉素( 5,000 µg/mL ) | Media Standard |
100 mL Penicillin-Streptomycin-L-Glutamine, 100x, 10,000 I.U. Penicillin (per mL) 10,000 µg/mL Streptomycin 29.2 mg/mL L-glutamine | 100x,青霉素-链霉素-谷氨酸钠 (10,000 I.U. 青霉素 (per mL) 10,000 µg/mL 链霉素 29.2 mg/mL 谷氨酸钠) | Media Standard |
50 mg Blasticidin S HCl | 灭瘟素 | Media Standard |
100 mL G418 Sulfate, Liquid 50 mg/mL Solution | G418硫酸 ( 50 mg/mL) | Media Standard |
20 mL G418 Sulfate, Liquid 50 mg/mL Solution | G418硫酸 ( 50 mg/mL) | Media Standard |
Hygromycin B Solution | 潮霉素 B 溶液 | Media Standard |
Corning® 500 mL Fetal Bovine Serum, Regular | 常规胎牛血清 | Serum |
Corning® 500 mL Donor Horse Serum, U.S. Sourced | 北美 供体马血清 | Serum |
Corning® 500 mL Fetal Bovine Serum, Premium (Gamma Irradiated) | 特优胎牛血清 | Serum |
500 mL SF Medium, with L-glutamine and 1 g/L BSA | SF培养基(含谷氨酸钠1 g/L BSA) | Specialty Products |
500 mL SF Medium with L-glutamine, 1 g/L BSA, without phenol red | SF培养基(含谷氨酸钠1 g/L BSA,不含酚红) | Specialty Products |
500 mL PF Medium without L-glutamine | PF 培养基(不含谷氨酸钠) | Specialty Products |
hybriGRO SF | hybriGRO SF培养基 | Specialty Products |
Corning® 100 mL Molecular Biology Grade Water Tested to USP Sterile Purified Water Specifications | 分子生物级水(USP级蒸馏纯净水) | Media Standard |
1L, Molecular Biology Grade Water; Tested to USP Sterile Purified Water Specifications | 分子生物级水(USP级蒸馏纯净水) | Media Standard |
500mL, Molecular Biology Grade Water; Tested to USP Sterile Purified Water Specifications | 分子生物级水(USP级蒸馏纯净水) | Media Standard |
4L,Reagent Grade Water; Tested to USP Purified Water Specifications | 试剂级水(USP级蒸馏纯净水) | Media Standard |
10 mL S.O.C. Medium | S.O.C. 培养基 | Media Standard |
1 L TE Buffer, 1x, Liquid | 1x TE 缓冲液 | Media Standard |
1 L TAE Buffer, 10x | 10x TAE缓冲液 | Media Standard |
1 L TBE Buffer, 10x, Liquid | 10x TBE缓冲液 | Media Standard |
1 L Tris Buffered Saline, 10x, Liquid | 10x Tris -cl缓冲液 | Media Standard |
1 L SSC Buffer, 20x | 20x SSC 缓冲液 | Media Standard |
1 L SSPE, 20x, Liquid | 20x SSPE 缓冲液 | Media Standard |
1 L 1M Tris-Hydrochloride Buffers, 1M Tris-HCI, pH 7.5, Liquid | 1M Tris-HCI缓冲液, pH 7.5 | Media Standard |
1 L 1M Tris-Hydrochloride Buffers, 1M Tris-HCI, pH 8.0, Liquid | 1M Tris-HCI缓冲液, pH 8.0 | Media Standard |
500 mL Sodium Chloride, 5M | 5M 氯化钠, | Media Standard |
100 mL Sodium Acetate, 3M | 3M 氯化钠 | Media Standard |
100 mL 0.5M EDTA, pH 8.0 | 0.5M EDTA, pH 8.0 | Media Standard |
100 mL SDS (Sodium Dodecyl Sulfate), 10% | 10% SDS溶液 | Media Standard |
1 L Miller's LB Broth | Miller's LB 肉汤 | Media Standard |
1 L Terrific Broth | Terrific 肉汤 | Media Standard |
100 mL Tryptic Soy Broth, animal free, Liquid | TBS, 无动物源性 | Specialty Products |
TSB (Tryptic Soy Broth), animal free, Liquid | TBS, 无动物源性 | Specialty Products |
20 L TSB (Tryptic Soy Broth), animal free, Liquid | TBS, 无动物源性 | Specialty Products |
热门标签:人戊肝抗体IgG HEV IgG ELISA 人戊肝抗体IgG试剂盒