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人神经保护因子(CVNPF)ELISA试剂盒*新闻快讯
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品牌名称：$brandModel.Title(进口品牌)型号：48T/96T 原产地：中国大陆 发布时间：2018/8/28 15:02:59更新时间：2024/12/26 15:06:59
产品摘要：人神经保护因子(CVNPF)ELISA试剂盒*新闻快讯为今品化学技术（上海）有限公司主打产品，质量好,发货及时,灵敏度高，特异性强，重复性好，其他动物类试剂盒种属一应俱全,指标数千种。公司配有专业实验室可寄样免费代检测支持现场观摩,提供售前售中售后全方位服务，欢迎来电询价或在线咨询索取产品说明书~
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详细内容
人神经保护因子(CVNPF)elisa试剂盒*新闻快讯操作步骤：
用于检测未知抗原的双抗体夹心法
1. 包被：用0.05MPH9.牰碳酸盐包被缓冲液将抗体稀释至蛋白质含量为1～10μg／ml。在每个聚苯乙烯板的反应孔中加0.1ml，4℃ 过夜。次日，弃去孔内溶液，用洗涤缓冲液洗3次，每次3分钟。（简称洗涤，下同）。
2. 加样：加一定稀释的待检样品0.1ml于上述已包被之反应孔中，置37℃ 孵育1小时。然后洗涤。（同时做空白孔，阴性对照孔及阳性对照孔）。
3. 加酶标抗体：于各反应孔中，加入新鲜稀释的酶标抗体（经滴定后的稀释度）0.1ml。37℃ 孵育0.5～1小时，洗涤。
4. 加底物液显色：于各反应孔中加入临时配制的TMB底物溶液0.1ml，37℃ 10～30分钟。
5. 终止反应：于各反应孔中加入2M硫酸0.05ml。
用于检测未知抗原的双抗体夹心法
1. 包被：用0.05MPH9.牰碳酸盐包被缓冲液将抗体稀释至蛋白质含量为1～10μg／ml。在每个聚苯乙烯板的反应孔中加0.1ml，4℃ 过夜。次日，弃去孔内溶液，用洗涤缓冲液洗3次，每次3分钟。（简称洗涤，下同）。
2. 加样：加一定稀释的待检样品0.1ml于上述已包被之反应孔中，置37℃ 孵育1小时。然后洗涤。（同时做空白孔，阴性对照孔及阳性对照孔）。
3. 加酶标抗体：于各反应孔中，加入新鲜稀释的酶标抗体（经滴定后的稀释度）0.1ml。37℃ 孵育0.5～1小时，洗涤。
4. 加底物液显色：于各反应孔中加入临时配制的TMB底物溶液0.1ml，37℃ 10～30分钟。
5. 终止反应：于各反应孔中加入2M硫酸0.05ml。
人神经保护因子(CVNPF)ELISA试剂盒*新闻快讯温馨说明：
1仔细阅读说明书
2确定试剂盒在有效期内
3按说明书确定所有试剂齐全（数量、体积）
4标本制备要规范，每份标本体积要按2-3个复孔以上的量制备（贮存），尽量分装做备份。短期内无法实验者，注意低温保存
5准备好所有实验额外所需物品，如试管、吸头、移液器、离心机等
6根据检测标本数量确定所需试剂的量
7按说明书将所用试剂平衡至室温，配制所需试剂。
3按说明书确定所有试剂齐全（数量、体积）
4标本制备要规范，每份标本体积要按2-3个复孔以上的量制备（贮存），尽量分装做备份。短期内无法实验者，注意低温保存
5准备好所有实验额外所需物品，如试管、吸头、移液器、离心机等
6根据检测标本数量确定所需试剂的量
7按说明书将所用试剂平衡至室温，配制所需试剂。
了解更多人神经保护因子(CVNPF)ELISA试剂盒*新闻快讯相关产品：
| 10-027-CVR | DMEM (Dulbecco＇s Modification of Eagle＇s Medium) with 25 mM HEPES, 4.5 g/L glucose, L-glutamine, without sodium pyruvate | DMEM培养基(HEPES,含谷氨酸钠，不含丙酮酸钠） | Media Standard |
| 10-040-CMR | RPMI 1640 with L-glutamine,1L | RPMI1640培养基 (含谷氨酰胺) | Media Standard |
| 10-040-LBR | 20 L RPMI 1640 with L-glutamine | RPMI1640培养基 (含谷氨酰胺) | Media Standard |
| 10-040-LXR | 10 L RPMI 1640 with L-glutamine | RPMI1640培养基 (含谷氨酰胺) | Media Standard |
| 10-041-CMR | 1 L RPMI 1640 with L-glutamine and 25 mM HEPES | RPMI1640培养基 (含谷氨酰胺，HEPES) | Media Standard |
| 10-043-CVR | 500 mL RPMI 1640 with L-glutamine, without glucose | RPMI1640培养基 (含谷氨酰胺,无糖) | Media Standard |
| 10-050-CVR | 500 mL McCoy＇s 5A (Iwakata & Grace Modification) with L-glutamine | 麦考伊5A(含谷氨酰胺) | Media Standard |
| 10-051-CIR | 100 mL McCoy＇s 5A (Iwakata & Grace Modification) with L-glutamine and 25 mM HEPES. | 麦考伊5A(含谷氨酰胺，HEPES) | Media Standard |
| 10-080-CMR | 1 L Ham＇s F-12 Medium with L-glutamine | Ham＇s F12 培养基 (含谷氨酰胺) | Media Standard |
| 10-090-CMR | 1 L DMEM (Dulbecco＇s Modification of Eagle＇s Medium)/Ham＇s F-12 50/50 Mix with L-glutamine | DMEM/F12培养基 (含谷酰胺) | Media Standard |
| 10-090-CVR | 500 mL DMEM (Dulbecco＇s Modification of Eagle＇s Medium)/Ham＇s F-12 50/50 Mix with L-glutamine | DMEM/F12培养基 (含谷酰胺) | Media Standard |
| 10-092-CMR | 1 L DMEM (Dulbecco＇s Modification of Eagle＇s Medium)/Ham＇s F-12 50/50 Mix with L-glutamine and 15 mM HEPES | DMEM/F12培养基 (含谷氨酰胺, HEPES) | Media Standard |
| 10-101-CVR | 500 mL DMEM (Dulbecco＇s Modification of Eagle＇s Medium), 1x with glutagro Supplement, 4.5 g/L glucose, sodium pyruvate and Phenol Red | DMEM培养基（含丙酮酸钠和苯酚红） | Media Standard |
| 10-102-CVR | 500 mL DMEM (Dulbecco＇s Modification of Eagle＇s Medium) with glutagrO, 4.5 g/L glucose, phenol red, without sodium pyruvate | DMEM培养基（含丙酮酸钠，不含苯酚红） | Media Standard |
| 10-103-CVR | 500 mL DMEM (Dulbecco＇s Modification of Eagle＇s Medium)/Ham＇s F-12 50/50 Mix with glutagro Supplement | DMEM/F12培养基 (含谷氨酰胺) | Media Standard |
| 10-104-CVR | 500 mL RPMI 1640, 1x with glutagro supplement and Phenol Red | RPMI1640培养基(含谷氨酰胺) | Media Standard |
| 13-100-CV | 500 mL Hink＇s TNM-FH Medium with L-glutamine lactalbumin hydrolysate, and yeastolate, without insect hemolymph | Hink＇s TNM-FH 培养基（不含昆虫淋巴液） | Specialty Products |
| 13-402-CV | 500 mL Insectagro DS2 Serum-Free/Protein-Free Medium, 1x without L-glutamine | Insectagro DS2 无血清/无蛋白培养基 1x 不含谷氨酸钠 | Specialty Products |
| 13-410-CV | 500 mL Insectagro Sf9 Serum-Free/Protein-Free Medium, with L-glutamine | Insectagro Sf9 无血清/无蛋白培养基，含谷氨酸钠 | Specialty Products |
| 13-901-CI | 100 mL Poloxamer 188 | 泊咯沙姆188 | Specialty Products |
| 15-010-CMR | 1 L MEM (Minimum Essential Medium) with Earle＇s salts, without L-glutamine | EME培养基（含Earle的盐，不含谷氨酰胺） | Media Standard |
| 15-010-CVR | 500 mL MEM (Minimum Essential Medium) with Earle＇s salts, without L-glutamine | EME培养基（含Earle的盐，不含谷氨酰胺） | Media Standard |
| 15-012-CVR | 500 mL MEM (Minimum Essential Medium) Alpha Medium with Earle＇s salts without ribonucleosides, deoxyribonucleosides, and L-glutamine | EME培养基（含Earle＇s盐无核糖、脱氧核糖核苷，不含谷氨酰胺 | Media Standard |
| 15-013-CMR | 1 L DMEM (Dulbecco＇s Modification of Eagles Medium) with 4.5 g/L glucose, sodium pyruvate, without L-glutamine | DMEM培养基 (高糖, 含丙酮酸钠，不含谷氨酰胺) | Media Standard |
| 15-013-LBR | 20 L DMEM (Dulbecco＇s Modification of Eagles Medium) with 4.5 g/L glucose, sodium pyruvate, without L-glutamine | DMEM培养基 (高糖, 含丙酮酸钠，不含谷氨酰胺) | Media Standard |
| 15-013-LXR | 10 L DMEM (Dulbecco＇s Modification of Eagles Medium) with 4.5 g/L glucose, sodium pyruvate, without L-glutamine | DMEM培养基 (高糖, 含丙酮酸钠，不含谷氨酰胺) | Media Standard |
| 15-015-CVR | 500 mL MEM (Minimum Essential Medium) (For Suspension Cultures) without L-glutamine, calcium, and magnesium | EME培养基（不含谷氨酰胺，钙，镁） | Media Standard |
| 15-016-CVR | Iscove＇s Modification of DMEM with 25 mM HEPES, without -thioglycerol, -mercaptoethanol, and L-glutamine | DMEM培养基 (含HEPES，不含谷氨酰胺) | Media Standard |
| 15-017-CMR | 1 L DMEM (Dulbeco＇s Modification of Eagle＇s Medium) with 4.5 g/L glucose, without L-glutamine, sodium pyruvate | DMEM培养基 (高糖, 不含谷氨酰胺，丙酮酸钠) | Media Standard |
| 15-018-CMR | 1 L DMEM (Dulbecco＇s Modification of Eagle＇s Medium) with 4.5 g/L glucose, 25 mM HEPES, sodium pyruvate, without L-glutamine | DMEM培养基 (含HEPES，丙酮酸钠，不含谷氨酰胺) | Media Standard |
| 15-018-CVR | 500 mL DMEM (Dulbecco＇s Modification of Eagle＇s Medium) with 4.5 g/L glucose, 25 mM HEPES, sodium pyruvate, without L-glutamine | DMEM培养基 (含HEPES，丙酮酸钠，不含谷氨酰胺) | Media Standard |
| 15-040-CMR | 1 L RPMI 1640 1x, without L-glutamine | RPMI1640培养基 (不含谷氨酰胺) | Media Standard |
| 15-040-LBR | 20 L RPMI 1640 1x, without L-glutamine | RPMI1640培养基 (不含谷氨酰胺) | Media Standard |
| 15-090-CMR | 1 L DMEM (Dulbecco＇s Modification of Eagle＇s Medium)/F12 50-50 Mix without L-glutamine | DMEM/F12培养基 (不含谷氨酰胺) | Media Standard |
| 15-100-CVR | 500 mL MCDB 131, 1x | MCDB 131(含谷氨酰胺) | Media Standard |
| 17-104-CIR | 100 mL RPMI 1640 without L-glutamine, L-methionine, and L-cystine | RPMI 1640(不含谷氨酰胺) | Media Standard |
| 17-105-CVR | 500 mL RPMI 1640 without L-glutamine and phenol red | RPMI 1640(不含谷氨酰胺，酚红) | Media Standard |
| 17-204-CIR | 100 mL DMEM (Dulbecco＇s Modification of Eagle＇s Medium) with 4.5 g/L glucose and sodium pyruvate without L-glutamine, L-methionine, and L-cystine | DMEM培养基 (不含谷氨酰胺，含丙酮酸钠) | Media Standard |
| 17-205-CVR | 500 mL DMEM (Dulbecco＇s Modification of Eagle＇s Medium) with 4.5 g/L glucose and sodium pyruvate without L-glutamine and phenol red | DMEM培养基 (不含谷氨酰胺，酚红，含丙酮酸钠) | Media Standard |
| 17-206-CIR | 100 mL DMEM (Dulbecco＇s Modification of Eagle＇s Medium) with 4.5 g/L glucose and sodium pyruvate without L-glutamine, and phosphate | DMEM培养基 (含丙酮酸钠，不含谷氨酰胺，磷酸盐） | Media Standard |
| 17-207-CVR | 500 mL DMEM (Dulbecco＇s Modification of Eagle＇s Medium), without glucose, L-glutamine, and sodium pyruvate | DMEM培养基 (不含谷氨酰胺，丙酮酸钠） | Media Standard |
| 17-305-CVR | 500 mL MEM (Minimum Essential Medium) with Earle＇s salts, without L-glutamine and phenol red | MEM培养基 (不含谷氨酰胺，酚红） | Media Standard |
| 20-021-CVR | 500 mL HBSS (Hank＇s Balanced Salt Solution), 10x without sodium bicarbonate, calcium and magnesium | HBSS平衡盐溶液 (不含碳酸氢铵、钙和镁) | Media Standard |
| 20-023-CVR | 500 mL HBSS (Hank＇s Balanced Salt Solution), 10x with calcium and magnesium without phenol red and sodium bicarbonate | HBSS平衡盐溶液 (含钙和镁、不含碳酸氢铵) | Media Standard |
| 20-030-CVR | 500 mL DPBS (Dulbecco＇s Phosphate-Buffered Saline), 10x with calcium and magnesium | DPBS缓冲液 (含钙, 镁) | Media Standard |
| 20-031-CVR | 500 mL DPBS (Dulbecco＇s Phosphate-Buffered Saline), 10x without calcium and magnesium | DPBS缓冲液 (不含钙, 镁) | Media Standard |
| 21-020-CMR | 1 L HBSS (Hank＇s Balanced Salt Solution), 1x with calcium and magnesium | HBSS平衡盐溶液 (含钙, 镁) | Media Standard |
| 21-021-CMR | 1 L HBSS (Hank＇s Balanced Salt Solution), 1x without calcium and magnesium | HBSS平衡盐溶液 (不含钙, 镁) | Media Standard |
| 21-022-CMR | 1 L HBSS (Hank＇s Balanced Salt Solution), 1x without calcium, magnesium and phenol red | HBSS平衡盐溶液 (不含钙, 镁, 酚红) | Media Standard |
| 21-023-CMR | 1 L HBSS (Hank＇s Balanced Salt Solution), 1x with calcium and magnesium without phenol red | HBSS平衡盐溶液 (含钙, 镁, 不含酚红) | Media Standard |
| 21-023-CVR | 500 mL HBSS (Hank＇s Balanced Salt Solution), 1x with calcium and magnesium without phenol red | HBSS平衡盐溶液 (含钙, 镁, 不含酚红) | Media Standard |
| 21-030-CMR | 1 L DPBS (Dulbecco＇s Phosphate-Buffered Saline), 1x with calcium and magnesium | DPBS缓冲液 (含钙, 镁) | Media Standard |
| 21-030-CVR | 500 mL DPBS (Dulbecco＇s Phosphate-Buffered Saline), 1x with calcium and magnesium | DPBS缓冲液 (含钙, 镁) | Media Standard |
| 21-031-CMR | 1 L DPBS (Dulbecco＇s Phosphate-Buffered Saline), 1x without calcium and magnesium | DPBS缓冲液 (不含钙, 镁) | Media Standard |
| 21-031-LBR | 20 L DPBS (Dulbecco＇s Phosphate-Buffered Saline), 1x without calcium and magnesium | DPBS缓冲液 (不含钙, 镁) | Media Standard |
| 21-031-LXR | 10 L DPBS (Dulbecco＇s Phosphate-Buffered Saline), 1x without calcium and magnesium | DPBS缓冲液 (不含钙, 镁) | Media Standard |
| 21-040-CMR | 1 L PBS (Phosphate-Buffered Saline), 1x without calcium and magnesium | PBS缓冲液 (不含钙, 镁) | Media Standard |
| 21-040-CMRX12 | 1 L PBS (Phosphate-Buffered Saline), 1x without calcium and magnesium | PBS), 1x 不含钙镁 | Media Standard |
| 25-005-CV | 500 mL L-Glutamine 200mM solution | 200mM谷氨酸钠溶液 | Media Standard |
| 25-020-CIR | 100 mL MEM Vitamins 100x Solution | MEM维生素（100X) | Media Standard |
| 25-021-CI | 100 mL Trace Elements A, 1000x | 微量元素 A, 1000x | Media Standard |
| 25-022-CI | 100 mL Trace Elements B, 1000x | 微量元素 B, 1000x | Media Standard |
热门标签：人神经保护因子 CVNPF ELISA 人神经保护因子试剂盒
