当前位置: 易推广 > 生物试剂/抗体/细胞 > 试剂盒 > 人ELISA试剂盒 > 今品化学技术(上海)有限公司 > 产品展示 > ELISA检测试剂盒 > 人ELISA检测试剂盒 > 人25羟维生素D3(25(OH)D3)ELISA试剂盒*新闻快讯
人25羟维生素D3(25(OH)D3)ELISA试剂盒*新闻快讯
企业档案
会员类型:会员
已获得易推广信誉 等级评定
(0 -40)基础信誉积累,可浏览访问
(41-90)良好信誉积累,可接洽商谈
(91+ )优质信誉积累,可持续信赖
易推广会员:7年
最后认证时间:
注册号: 【已认证】
法人代表: 【已认证】
企业类型:经销商 【已认证】
注册资金:人民币万 【已认证】
产品数:8783
参观次数:2814977
详细内容
中文名:人25羟维生素D3(25(OH)D3)elisa试剂盒*新闻快讯
英文名:human 25(OH)D3 ELISA Kit
市场规格:96T/48T
制备方法包括以下步骤:
1. 抗原表位的计算机筛选;
2. 抗原的制备;
3. 血清的采集;
4. 间接ELISA方法的建立;
5. 间接ELISA方法的敏感性和特异性验证;
6. 试剂盒的稳定性验证;
人25羟维生素D3(25(OH)D3)ELISA试剂盒*新闻快讯使用注意事项:
1.试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存。
2.浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果。
3.各步加样均应使用加样器,并经常校对其准确性,以避免试验误差。一次加样时间控制在5分钟内,如标本数量多,推荐使用排枪加样。
4.请每次测定的同时做标准曲线,做复孔。如标本中待测物质含量过高(样本OD值大于标准品孔孔的OD值),请先用样品稀释液稀释一定倍数(n倍)后再测定,计算时请*后乘以总稀释倍数(×n×5)。
5.封板膜只限一次性使用,以避免交叉污染。
6.底物请避光保存。
7.严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.
8.所有样品,洗涤液和各种废弃物都应按传染物处理。
9.本试剂不同批号组分不得混用。
10. 如与英文说明书有异,以英文说明书为准。
附:采用全自动酶标仪检测,只需检测前设置好酶标仪的相关参数,如坐标参数(线性,半对数)和计算方式参数(三次回归、四参数或双对数曲线方程),即可直接得到结果。
存放:收到货后贮存于4℃,强烈建议尽快使用配制的试剂1X缓冲液贮存于4℃,将不用的微孔板放回至密封袋内密封起来,贮存于4℃,已稀释的标准品,生物标记物,抗体和HRP贮存于4℃。
人25羟维生素D3(25(OH)D3)ELISA试剂盒*新闻快讯其他相关产品:
IL-15 Polyclonal Antibody | P40933 | H | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | Interleukin-15 (IL-15) | |||
OPN Polyclonal Antibody | P10451 | H, M, R | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | Osteopontin (Bone sialoprotein 1) (Nephropontin) (Secreted phosphoprotein 1) (SPP-1) (Urinary stone protein) (Uropontin) | |||
Epiregulin Polyclonal Antibody | O14944 | H, M, R | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | Proepiregulin [Cleaved into: Epiregulin (EPR)] | |||
MCP-3 Polyclonal Antibody | P80098 | H | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | C-C motif chemokine 7 (Monocyte chemoattractant protein 3) (Monocyte chemotactic protein 3) (MCP-3) (NC28) (Small-inducible cytokine A7) | |||
IL-17F Polyclonal Antibody | Q96PD4 | H | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | Interleukin-17F (IL-17F) (Cytokine ML-1) (Interleukin-24) (IL-24) | |||
IL-17D Polyclonal Antibody | Q8TAD2 | H | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | Interleukin-17D (IL-17D) (Interleukin-27) (IL-27) | |||
Granzyme M Polyclonal Antibody | P51124 | H | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | Granzyme M (EC 3.4.21.-) (Met-1 serine protease) (Hu-Met-1) (Met-ase) (Natural killer cell granular protease) | |||
EYA1/EYA4 Polyclonal Antibody | Q99502/O95677 | H, M | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | Eyes absent homolog 3/4 | |||
CNPY3 Polyclonal Antibody | Q9BT09 | H, M | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | Protein canopy homolog 3 (CTG repeat protein 4a) (Expanded repeat-domain protein CAG/CTG 5) (Protein associated with TLR4) (Trinucleotide repeat-containing gene 5 protein) | |||
NAIP Polyclonal Antibody | Q13075 | H, M | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | Baculoviral IAP repeat-containing protein 1 (Neuronal apoptosis inhibitory protein) | |||
MYH6/MYH7 Polyclonal Antibody | P13533/P12883 | H, M, R | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | Myosin-6 (Myosin heavy chain 6) (Myosin heavy chain, cardiac muscle alpha isoform) (MyHC-alpha) Myosin-7B (Antigen MLAA-21) (Myosin cardiac muscle beta chain) (Myosin heavy chain 7B, cardiac muscle beta isoform) | |||
JAK2/3 (Phospho-Tyr966/939) Antibody | O60674/P52333 | H:Y966/939, M:Y966/935, R:Y966/935 | wb dilution 1:1000 icc dilution 1:200 | Rabbit | JAK2/3 | |||
CTXL Polyclonal Antibody | Q96IQ7 | H | wb dilution 1:1000 icc dilution 1:400 ip dilution 1:100 | Rabbit | CTXL | |||
GLI-1 Polyclonal Antibody | P08151 | H, M | wb dilution 1:2000 | Rabbit | GLI-1 | |||
LIM and SH3 domain protein 1 Polyclonal Antibody | Q14847 | H, M, R | wb dilution 1:1000 icc dilution 1:200 ihc dilution 1:800 | Rabbit | LIM and SH3 domain protein 1 | |||
ACSS1 (Acetyl-K642) Polyclonal Antibody | Q9NUB1 | H:K642, M:K635, R:K635 | wb dilution 1:1000 icc dilution 1:200 | Rabbit | ACSS1 | |||
AR(Acetyl-K631) Polyclonal Antibody | P10275 | H:K631, M:K610, R:K613 | wb dilution 1:2000 icc dilution 1:200 | Rabbit | AR | |||
E2F-1 (Acetyl-K117) Polyclonal Antibody | Q01094 | H:K117, M:K112, R:K115 | wb dilution 1:2000 | Rabbit | E2F-1 | |||
E2F-1 (Acetyl-K125) Polyclonal Antibody | Q01094 | H:K125, M:K120, R:K123 | wb dilution 1:1000 icc dilution 1:200 | Rabbit | E2F-1 | |||
p53 (Acetyl-K120) Polyclonal Antibody | P04637 | H:K120, M:K114, R:K118 | wb dilution 1:1000 icc dilution 1:200 | Rabbit | p53 | |||
p53 (Acetyl-K305) Polyclonal Antibody | P04637 | H:K305 | wb dilution 1:1000 | Rabbit | p53 | |||
PARP-1 (Acetyl-K521) Polyclonal Antibody | P09874 | H:K521, M:K520, R:K521 | wb dilution 1:1000 | Rabbit | PARP-1 | |||
Rb (Acetyl-K873/K874) Polyclonal Antibody | P06400 | H:K873+K874, M:K866+K867, R:K865+K866 | wb dilution 1:1000 | Rabbit | Rb | |||
Histone H3 (Tri-Methyl-K5) Polyclonal Antibody | P68431/Q71DI3/P84243/Q6NXT2 | H:K5 M:K5 R:K5 | wb dilution 1:1000 icc dilution 1:100 ip dilution 1:100 | Rabbit | Histone H3 | |||
Histone H3 (Di-Methyl-K80) Polyclonal Antibody | P68431/Q71DI3/P84243/Q6NXT2 | H:K80 M:K80 R:K80 | wb dilution 1:1000 | Rabbit | Histone H3 | |||
Cardiotrophin-1 Polyclonal Antibody | Q16619 | H, M, R | wb dilution 1:1000 | Rabbit | Cardiotrophin-1 | |||
NUT Polyclonal Antibody | Q86Y26 | H | WB 1:500-2000, ELISA 1:10000-20000 | Rabbit | NUT | |||
Thioredoxin Polyclonal Antibody | P10599 | H, M, R | WB 1:500-2000, ELISA 1:10000-20000 | Rabbit | Thioredoxin | |||
Gl Syn Polyclonal Antibody | P15104 | H, M, R | wb dilution 1:50000 | Rabbit | Gl Syn | |||
Parafibromin Polyclonal Antibody | Q6P1J9 | H, M, R | wb dilution 1:1000 | Rabbit | Parafibromin | |||
FIG4 Polyclonal Antibody | Q92562 | H, M | wb dilution 1:1000 | Rabbit | FIG4 | |||
KCTD7 Polyclonal Antibody | Q96MP8 | H, M, R | WB 1:500-2000, ELISA 1:10000-20000 | Rabbit | KCTD7 | |||
GRIN1 Polyclonal Antibody | Q05586 | H M R | wb dilution 1:2000 icc dilution 1:400 | Rabbit | GRIN1 | |||
LWS Polyclonal Antibody | P48443 | H M R | wb dilution 1:2000 icc dilution 1:400 | Rabbit | LWS | |||
Collagen I Polyclonal Antibody | P08123 | H M R | wb dilution 1:1000 | Rabbit | Collagen I | |||
TNFAIP8L3 Polyclonal Antibody | Q5GJ75 | H M R | wb dilution 1:1000 | Rabbit | TNFAIP8L3 | |||
FA2H Polyclonal Antibody | 55 | Q7L5A8 | H, M, R | WB 1:500-2000, ELISA 1:10000-20000 | Rabbit | FA2H | ||
V-ATPase S1 Polyclonal Antibody | 51 | Q15904 | H, M, R | WB 1:500-2000, ELISA 1:10000-20000 | Rabbit | V-ATPase S1 | ||
Integrin β7 Polyclonal Antibody | 130 | P26010 | H,M | WB 1:500-2000, ELISA 1:10000-20000 | Rabbit | Integrin β7 | ||
Dlx-2 Polyclonal Antibody | 42 | Q07687 | H, M, R | WB 1:500-2000, ELISA 1:10000-20000 | Rabbit | Dlx-2 | ||
PBEF Polyclonal Antibody | 54 | P43490 | H, M, R | WB 1:500-2000, ELISA 1:10000-20000 | Rabbit | PBEF | ||
COL8A2 Polyclonal Antibody | 80 | P25067 | H, M, R | WB 1:500-2000, ELISA 1:10000-20000 | Rabbit | COL8A2 | ||
GCM2 Polyclonal Antibody | 56 | O75603 | H, M, R | WB 1:500-2000, ELISA 1:10000-20000 | Rabbit | GCM2 |