TNF-α/ IL-1β/IL-18/MDA/SOD/LDHELISA试剂盒文献
 Epimedium Aqueous Extract Ameliorates Cerebral Ischemia/Reperfusion Injury through Inhibiting ROS/NLRP3- Mediated Pyroptosis
staining blocking buffer (P0102, Beyotime, Shanghai, China). Then, the sections were incubated with the primary antibodies overnight at 4 ◦C; after incubation, sections were again incubated with the corresponding secondary antibody. The antibodies used in this manuscript were glial fibrillary acidic protein (GFAP, 1:50, ab7260, Abcam, Cambridge, UK), ionized calcium-binding adaptor molecule 1 (Iba-1, 1:50, ab178847, Abcam), Neuron (NeuN, 1:100, 66836-1-Ig, Proteintech, Wuhan, China), ａnd NLRP3 (1:100, 27458-1-AP, Proteintech). After 4′ ,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich) staining, the result was observed by fluorescence microscope (BX 53 Olympus, Tokyo, Japan). 2.13. Enzyme-Linked Immunosorbent Assay (ELISA) Serum levels of IL-1β (RJ15465, Renjiebio, Shanghai, China), IL-18 (RJ15463, Renjiebio), TNF-α (RJ16622, Renjiebio), IL-6 (RJ15478, Renjiebio), IL-10 (RJ15453, Renjiebio), inducible nitric oxide synthase (iNOS, RJ16565, Renjiebio), malondialdehyde (MDA, RJ15503, Renjiebio), reactive oxygen species (ROS, RJ15780, Renjiebio), catalase (CAT, RJ15737, Renjiebio), superoxide dismutase (SOD, RJ16691, Renjiebio), ａnd glutathione peroxidase (GSH-Px, RJ25745, Renjiebio) were detected using conventional ELISA kits according to the manufacturer’s instructions. In addition, lactate dehydrogenase (LDH) released in rat serum was measured to assess cell damage using the LDH test kit (RJ16172, Renjiebio) according to the manufacturer’s instructions

原创作者：上海仁捷生物科技有限公司