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HORIBA 经济型荧光寿命LRET系统
点击次数：123发布时间：2020/4/17 14:30:12
更新日期：2020/4/17 14:30:12
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详细内容
技术参数 | |
| 寿命范围 | From 150 ps to 3 μs |
| 仪器尺寸 | 15" x 13" x 8.8" (WxDxH) |
| 重量 | 25 lbs |
| 灵敏度 | 400 pM fluorescein or better |
| 激发光源 | OBB proprietary pulsed nanosecond LEDs |
| 脉冲宽度 | 1.5 ns (typical) |
| 激发光谱范围 | 280–670 nm (LED dependent) |
| 发射光谱范围 | 200–650 nm (optional to 900 nm) |
| 激发波长选择 | By filters |
| 探测器 | OBB patented lifetime detector |
| 采集时间 | 20 s (sample dependent) |
| Timescale (menu selectable) | Linear, arithmetic and logarithmic |
| 采集模式 | Fluorescence Decay or Lifetime Kinetics |
| 样品仓 | Single 1 x 1 cm cuvette (micro-cuvettes available) |
| 软件 | EasyLife? X |
| 寿命分析 | Complete package: 1-to-4 exp, global, non-exponential, micelle kinetics, lifetime distribution (ESM, MEM), anisotropy, FRET calculator |
| QuickStart DVD | Included |
脉冲LED光源
Nothing could be easier. OBB engineers have developed a broad range of simple, inexpensive, nanosecond pulsed light emitting diodes (LED's) to be used as excitation sources with the EasyLife? systems. These small but robust light sources are available in a broad range of wavelengths, from UV to NIR. They have excellent pulse to pulse, and long term, stability in their intensity as well as their temporal pulse profiles as is evidenced by the high quality of data the system produces. Switching from one LED to another requires no alignment, and takes only a few seconds. You could not possibly work with any simpler, maintenance-free, light source for fluorescence lifetime research.
脉冲LED的优势
The EasyLife? pulsed LEDs have certain distinct advantages over more conventional light sources, such as nanosecond flash lamps and lasers.
These advantages are:
Inexpensive
Durable
Require no maintenance
Very stable pulse sources
Fast
Wavelengths from UV to NIR (currently 280–670 nm)
Small size
Easily interchangeable (snap in—snap out)
Impressive Pulse Stability
 |  |
6 Hours Repeat Acquisition | Repeat After 4 Days |
Click on images to enlarge. | |
Available LEDs
What you need to do is to select a source or sources. The following list shows you the current choices. To exchange sources is real easy, just snap off and then snap on and it is done—no alignment required.
Below 280 nm we are limited by the current state of the technology for LED’s. Do not despair the future should offer even more choices.
LED Sources: | Central Wavelength |
EL 265 | excitation LED: 266 +\- 10 nm |
EL 278 | excitation LED: 280 +\- 10 nm |
EL 295 | excitation LED: 297 +\- 10 nm |
EL 310 | excitation LED: 310 +\- 10 nm |
EL 340 | excitation LED: 340 +\- 10 nm |
EL 366 | excitation LED: 368 +\- 10 nm |
EL 375 | excitation LED: 375 +\- 10 nm |
EL 405 | excitation LED: 403 +\- 10 nm |
EL 410 | excitation LED: 407 +\- 10 nm |
EL 435 | excitation LED: 432 +\- 10 nm |
EL 445 | excitation LED: 444 +\- 10 nm |
EL 450 | excitation LED: 456 +\- 10 nm |
EL 490 | excitation LED: 486 +\- 10 nm |
EL 505 | excitation LED: 510 +\- 10 nm |
EL 525 | excitation LED: 518 +\- 10 nm |
EL 570 | excitation LED: 572 +\- 10 nm |
LD 630 | excitation laser diode: 633 +\- 10 nm |
LD 650 | excitation laser diode: 649 +\- 10 nm |
LD 670 | excitation laser diode: 667 +\- 10 nm |
Other LED's and laser diodes are available on request. | |
