人β-联蛋白β-CateninELISA试剂盒@2022资料已更新
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一、人β-联蛋白β-CateninELISA试剂盒@2022资料已更新实验原理

人β-联蛋白β-CateninELISA试剂盒是采用双抗体一步夹心法酶联免疫吸附试验（elisa）。往预先包被人人β-联蛋白β-Catenin捕获抗体的包被微孔中，依次加入标本、标准品、HRP标记的检测抗体，经过温育并洗涤。用底物TMB显色，TMB在化物酶的催化下转化成蓝色，并在酸的作用下转化成zui终的黄色。颜色的深浅和样品中的人人β-联蛋白β-Catenin抗体呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样品浓度。

二、产品信息

指标名称：人β-联蛋白β-CateninELISA试剂盒

规格：48T/ 96T

品牌：研启生物

检测波长：450nm

所需样本体积：量50ul

适用范围：仅供科研课题研究，不得用于临床诊断。

检测样本种类：血清，血浆，尿液，组织匀浆，细胞上清液，脑脊液，灌洗液，粪便等样本.

臻科供应属种有：人、大鼠、小鼠、猴子、猪牛羊、犬、鸡鸭鹅、植物、鱼、昆虫ELISA试剂盒等

试剂盒保存：2-8℃冷藏保存，保质期6个月。

三、人β-联蛋白β-CateninELISA试剂盒@2022资料已更新独特优势

1、我司产品具有灵敏度高、特强、重复性好等特点，吸附性能好,空白值低，并适用于血清血浆 组织匀浆等多种样本类型的检测，方法简便，操作简单。 

2、产品质量可靠，出库质检把控严格，顺丰快递，运输全程跟踪，有任何质量问题包退换，诚信经营，合作共赢。  

3、凡是订购我司任何一款ELISA试剂盒，享全程技术指导并可免费代测。全程有业技术老师负责代测，收到样本一般5个工作日出结果。

4、我司具有完善的售后服务体系，设立售后服务中心，24小时及时响应，真正做到后顾无忧，不断完善服务体系。

四、标本处理要求

1.血清：室温血液自然凝固10-20分钟，离心20分钟左右（2000-3000转/分）。仔细收集上清，保存过程中如出现沉淀，应再次离心。

2.血浆：应根据标本的要求选择EDTA或柠檬酸钠作为抗凝剂，混合10-20分钟后，离心20分钟左右（2000-3000转/分）。仔细收集上清，保存过程中如有沉淀形成，应该再次离心。

3.尿液：用无菌管收集，离心20分钟左右（2000-3000转/分）。仔细收集上清，保存过程中如有沉淀形成，应再次离心。胸腹水、脑脊液参照实行。

4.细胞培养上清：检测分泌性的成份时，用无菌管收集。离心20分钟左右（2000-3000转/分）。仔细收集上清。检测细胞内的成份时，用PBS（PH7.2-7.4）稀释细胞悬液，细胞浓度达到100万/ml左右。通过反复冻融，以使细胞破坏并放出细胞内成份。离心20分钟左右（2000-3000转/分）。仔细收集上清。保存过程中如有沉淀形成，应再次离心。

5. 组织标本：切割标本后，称取重量。加入一定量的PBS，PH7.4。用液氮迅速冷冻保存备用。标本融化后仍然保持2-8℃的温度。加入一定量的PBS（PH7.4），用手工或匀浆器将标本匀浆充分。离心20分钟左右（2000-3000转/分）。仔细收集上清。分装后一份待检测，其余冷冻备用。

五、人β-联蛋白β-CateninELISA试剂盒@2022资料已更新操作流程

1.从室温平衡20min后的铝箔袋中取出所需板条，剩余板条用自封袋密封放回4℃。

2.设置标准品孔和样本孔，标准品孔各加不同浓度的标准品50μL；

3.样本孔中加入待测样本50μL；空白孔不加。

4.除空白孔外，标准品孔和样本孔中每孔加入辣根化物酶（HRP）标记的检测抗体100μL，用封板膜封住反应孔，37℃水浴锅或恒温箱温育60min。

5.弃去液体，吸水纸上拍干，每孔加满洗涤液（350μL），静置1min，甩去洗涤液，吸水纸上拍干，如此重复洗板5次（也可用洗板机洗板）。

6.每孔加入底物A、B各50μL，37℃避光孵育15min。

7.每孔加入终止液50μL，15min内，在450nm波长处测定各孔的OD值。

六、特别说明

上海研启生物科技有限公司供应各类科研产品，包括elisa试剂盒、农残试剂盒、生化试剂盒、生物试剂、检测卡等高质量产品。公司本着客户至上的原则为客户提供高品质的可靠产品，如有任何质量的问题请打电话与我司工作人员联系,免费包退换（非人为因素造成的）。公司产品优惠活动进行中，欢迎新老客户来选购。
特别提醒：

1.试剂盒保存在2-8℃，使用室温平衡20分钟。从冰箱取出的浓缩洗涤液会有结晶，这属于正常现象，水浴加热使结晶溶解后再使用。

2.实验中不用的板条应立即放回自封袋中，密封（低温干燥）保存。

3.浓度为0的S0号标准品即可视为阴性对照或者空白；按照说明书操作时样本已经稀释5倍，终结果乘以5才是样本实际浓度。

4.严格按照说明书中标明的时间、加液量及顺序进行温育操作。

5.所有液体组分使用充分摇匀。

1、 人β-联蛋白β-CateninELISA试剂盒experimental principle

人β-联蛋白β-CateninELISA试剂盒 is enzyme-linked immunosorbent assay (ELISA) with double antibody one-step sandwich method. Add samples, standards, ａnd HRP labeled test antibodies to the coating wells that are pre coated with human 人β-联蛋白β-Catenin captured antibodies, incubate them ａnd wash them thoroughly. With the substrate TMB for color development, TMB is converted into blue under the catalysis of peroxidase, ａnd into Zui yellow under the action of acid. The depth of color is positively correlated with the human 人β-联蛋白β-Catenin antibody in the sample. Determine the absorbance (OD value) with a microplate reader at the welength of 450nm ａnd calculate the sample concentration.

2、 Product information

Index name: 人β-联蛋白β-CateninELISA试剂盒

Specification: 48t/ 96T

Brand: Yanqi biology

Detection welength: 450nm

Required sample volume: Insurance volume 50ul

Scope of application: only for scientific research, not for clinical diagnosis.

Types of test samples: serum, plasma, urine, tissue homogenate, cell supernatant, cerebrospinal fluid, lage fluid, feces ａnd other samples

The genus ａnd species supplied by Zhenke include: human, rat, mouse, monkey, pig, cattle, sheep, dog, chicken, duck, goose, plant, fish, insect ELISA kit, etc

Storage of Kit: refrigerate at 2-8 ℃, with a shelf life of 6 months.

3、人β-联蛋白β-CateninELISA试剂盒@2022资料已更新 unique advantages

1. Our products he the characteristics of high sensitivity, strong specificity, good repeatability, good adsorption performance, low blank value, ａnd are suitable for the detection of serum, plasma, tissue homogenate ａnd other sample types. The method is simple ａnd the operation is simple.

2. The product quality is reliable, the quality inspection of delivery is strictly controlled, SF express, the whole process of transportation is tracked, any quality problems are returned ａnd replaced, honest operation, ａnd win-win cooperation.

3. Anyone who orders any of our ELISA kits can enjoy the whole process of technical guidance ａnd free proxy testing. The professional technical teacher is responsible for the surrogate test throughout the whole process, ａnd the results are generally released within 5 working days after receiving the samples.

4. Our company has a perfect after-sales service system, set up an after-sales service center, respond in a timely manner 24 hours, truly worry free, ａnd constantly improve the service system.

4、 Specimen handling requirements

1. Serum: Blood naturally coagulates at room temperature for 10-20 minutes ａnd centrifuges for about 20 minutes (2000-3000 RPM). Collect the supernatant carefully. If there is precipitation during storage, centrifuge again.

2. Plasma: EDTA or sodium citrate should be selected as the anticoagulant according to the requirements of the sample. After mixing for 10-20 minutes, centrifuge for about 20 minutes (2000-3000 RPM). Collect the supernatant carefully. If there is sediment formation during storage, it should be centrifuged again.

3. Urine: collect with sterile tube ａnd centrifuge for about 20 minutes (2000-3000 RPM). Collect the supernatant carefully. If there is precipitation during storage, centrifuge again. Pleural effusion, ascites ａnd cerebrospinal fluid shall be implemented by reference.

4. Cell culture supernatant: collect with sterile tube when detecting secretory components. Centrifuge for about 20 minutes (2000-3000 RPM). Carefully collect the supernatant. When detecting the components in cells, dilute the cell suspension with PBS (ph7.2-7.4), ａnd the cell concentration reaches about 1million /ml. Through repeated freezing ａnd thawing, the cells are destroyed ａnd intracellular components are released. Centrifuge for about 20 minutes (2000-3000 RPM). Carefully collect the supernatant. If sediment is formed during storage, it should be centrifuged again.

5. Tissue specimen: weigh the specimen after cutting. Add a certain amount of PBS, pH7.4. Quickly freeze with liquid nitrogen for standby. The specimen remains at a temperature of 2-8 ℃ after melting. Add a certain amount of PBS (pH7.4) ａnd homogenize the specimen fully by hand or homogenizer. Centrifuge for about 20 minutes (2000-3000 RPM). Carefully collect the supernatant. After subpackaging, one copy is to be tested, ａnd the rest is frozen for standby.

5、 [title] operation process

1. Take out the required strips from the aluminum foil bag after the room temperature is balanced for 20min, ａnd seal the remaining strips with a self sealing bag ａnd put them back at 4 ℃.

2. Set the standard hole ａnd sample hole, ａnd add standard 50 of different concentrations to the standard hole μ L；

3. Add 50 samples to be tested into the sample hole μ L； Blank hole is not added.

4. In addition to the blank hole, add horseradish peroxidase (HRP) labeled detection antibody 100 to each hole in the standard hole ａnd sample hole μ 50. Seal the reaction hole with a sealing film, ａnd incubate in a 37 ℃ water bath or incubator for 60min.

5. Discard the liquid, pat it dry on the absorbent paper, ａnd fill each hole with detergent (350 μ 50) Let it stand for 1min, throw away the washing liquid, pat it dry on the absorbent paper, ａnd repeat washing the plate for 5 times (you can also use the plate washing machine to wash the plate).

6. Add 50 substrates A ａnd B to each hole μ 50. Incubate at 37 ℃ away from light for 15min.

7. Add termination liquid 50 to each hole μ 50. Measure the OD value of each hole at the welength of 450nm within 15min.

6、 Special instructions

Shanghai Yanqi Biotechnology Co., Ltd. supplies all kinds of scientific research products, including ELISA kits, pesticide residue kits, biochemical kits, biological reagents, test cards ａnd other high-quality products. Based on the principle of customer first, the company provides customers with high-quality ａnd reliable products. If you he any quality problems, please call our staff ａnd return them for free (not caused by human factors). The company＇s product preferential activities are in progress. New ａnd old customers are welcome to buy.

Special reminder:

1. Keep the kit at 2-8 ℃ and balance the room temperature for 20 minutes before use. The concentrated detergent taken out from the refrigerator will crystallize, which is a normal phenomenon. It can be used after the crystallization is completely dissolved by heating in the water bath.

2. The strips not used in the experiment should be immediately put back into the self sealing bag ａnd sealed (dry at low temperature) for storage.

3. S0 standard with concentration of 0 can be regarded as negative control or blank; When operating according to the instructions, the sample has been diluted 5 times, ａnd the final result multiplied by 5 is the actual concentration of the sample.

4. The incubation operation shall be carried out in strict accordance with the time, liquid dosage ａnd sequence indicated in the instructions.

5. Shake all liquid components well before use.

人β痕迹蛋白PELISA试剂盒2022报价已更新

原创作者：上海研启生物科技有限公司