产品名称：禽流感（通用、H5型）检测试剂盒(双色实时荧光PCR法)
通用名称：禽流感（通用、H5型）检测试剂盒(双色实时荧光PCR法)

包装规格：50T/盒

储存及有效期：避光-20℃以下保存，避免反复冻融，有效期为12个月。

适用仪器 ： ABI 系列、Bio-Rad系列、Agilent Stratagene MX系列 、Roche LightCycler R480、Cepheid SmartCycler、Rotor-Gene系列、杭州博日系列等多通道实时荧光定量PCR仪

实时荧光PCR的原理
实时荧光定量PCR（Quantitative Real-time PCR）是一种在DNA扩增反应中，以荧光化学物质测每次聚合酶链式反应（PCR）循环后产物总量的方法。通过内参或者外参法对待测样品中的特定DNA序列进行定量分析的方法。

Real-timePCR是在PCR扩增过程中，通过荧光信号，对PCR进程进行实时检测。由于在PCR扩增的指数时期，模板的Ct值和该模板的起始拷贝数存在线性关系，所以成为定量的依据。

禽流感（通用、H5型）检测试剂盒(双色实时荧光PCR法)特点

1.灵敏度高，操作简便。

2.节省时间。  

3.准确性高，特好，假阳性低。

4.一管式荧光定量pcr检测，避免后续污染。

5.对原始材料质t要求低。

6.仅供科研不得用于临床诊断使用。

试剂盒的判定

1.试剂盒有效性判定：

（1）阳性对照：有典型S型扩增曲线或Ct值≤35。

（2）阴性对照：Ct值＞38或无Ct值，线形为直线或轻微斜线，无指数增长期。

2.标本结果判定：

（1）阳性：标本检测结果Ct值≤35或有明显指数增长期。

（2）可疑：标本检测结果Ct值在35～38范围内。此时应对标本进行重复检测，如重复实验结果Ct值仍在35～38范围内，有明显指数增长期，则判定为阳性，否则为阴性。

（3）阴性：标本检测结果Ct值＞38或无Ct值。

禽流感（通用、H5型）检测试剂盒(双色实时荧光PCR法)注意事项

1.整个检测过程应严格按照本说明书要求分别在试剂准备区、样本处理区和PCR扩增区进行操作，各区实验服、仪器、耗材应独立使用，不能混用；实验用吸头采用带滤芯吸头；样本处理区应配有生物安全柜，样本处理在生物安全柜中进行操作；三个区应该配有紫外线杀菌装置。

2.每次实验应该设置阴、阳性对照。

3.为保证试剂不受标本污染，必须将混合酶液及PCR反应液保存于试剂准备区。

4.试剂盒所有试剂在使用应该在常温下充分融化混匀，并应瞬时离心。

5.试剂盒内所配阴性和阳性对照在di一次使用应全部转移至标本准备区，并单独存放。

6.为防止荧光干扰，应避免裸手直接接触PCR反应管，并应避免在PCR反应管上进行任何标记。
7.仪器扩增相关参数应按照本说明书相关要求进行设置；不同批号试剂不能混用。

8.ABI系列荧光定量PCR仪参数设置中不选ROX校正，淬灭基因选择None。

9.实验过程中产品的废弃物应该进行无害化处理后方可丢弃。

更多详细信息请来电咨询，期待与您的合作共赢。

1. The whole testing process should be operated in the reagent preparation area, sample processing area ａnd PCR amplification area in strict accordance with the requirements of this manual. The laboratory clothes, instruments ａnd consumables in each area should be used independently ａnd cannot be mixed; The suction head with filter element is used in the experiment; The sample processing area shall be equipped with a biosafety cabinet in which the sample processing is conducted; The three zones should be equipped with ultriolet sterilization devices.

2. Negative ａnd positive controls should be set for each experiment.

3. To ensure that the reagent is not contaminated by the sample, the mixed enzyme solution ａnd PCR reaction solution must be stored in the reagent preparation area.

4. All reagents in the kit should be fully melted ａnd mixed at room temperature before use, ａnd should be centrifuged instantaneously.

5. All the negative ａnd positive controls in the kit should be transferred to the sample preparation area ａnd stored separately before the first use of di.

6. In order to prevent fluorescence interference, it is necessary to oid direct contact of bare hands with the PCR reaction tube ａnd oid any marking on the PCR reaction tube.

7. Relevant parameters of instrument amplification shall be set according to relevant requirements of this manual; Reagents of different batch numbers cannot be mixed.

8. In the parameter setting of ABI series fluorescence quantitative PCR instrument, ROX correction is not selected, ａnd None is selected for quenching gene.

9. During the experiment, the waste of the product should be disposed innocuously before being discarded.

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