µ±Ç°Î»Ö㺠Ò×Íƹã > ÉúÎïÊÔ¼Á/¿¹Ìå/ϸ°û > ÊÔ¼ÁºÐ > Å£ELISAÊÔ¼ÁºÐ > ÉϺ£ÑÐÆôÉúÎï¿Æ¼¼ÓÐÏÞ¹«Ë¾ > ²úƷչʾ > ELISAÊÔ¼ÁºÐ > Å£ELISA¼ì²âÊÔ¼ÁºÐ > Å£Ó²Ö¬õ£¸¨Ã¸AÈ¥±¥ºÍø(SCD£©ELISA¼ì²âÊÔ¼ÁºÐ
Å£Ó²Ö¬õ£¸¨Ã¸AÈ¥±¥ºÍø(SCD£©ELISA¼ì²âÊÔ¼ÁºÐ
¼Û¸ñ£º£¤µçÒé
Æ·ÅÆÃû³Æ£º$brandModel.Title(½ø¿ÚÆ·ÅÆ)Ðͺţº Ô²úµØ£ºÖйú´ó½ ·¢²¼Ê±¼ä£º2020/9/9 11:01:21¸üÐÂʱ¼ä£º2024/10/23 13:32:22
²úÆ·ÍêÉƶȣº ·ÃÎÊ´ÎÊý£º1146
ÆóÒµµµ°¸
»áÔ±ÀàÐÍ£º»áÔ±
ÒÑ»ñµÃÒ×ÍƹãÐÅÓþ µÈ¼¶ÆÀ¶¨
(0 -40)»ù´¡ÐÅÓþ»ýÀÛ£¬¿Éä¯ÀÀ·ÃÎÊ
(41-90)Á¼ºÃÐÅÓþ»ýÀÛ£¬¿É½ÓÇ¢ÉÌ̸
(91+ )ÓÅÖÊÐÅÓþ»ýÀÛ£¬¿É³ÖÐøÐÅÀµ
Ò×Íƹã»áÔ±£º5Äê
×îºóÈÏ֤ʱ¼ä£º
×¢²áºÅ£º ¡¾ÒÑÈÏÖ¤¡¿
·¨ÈË´ú±í£º ¡¾ÒÑÈÏÖ¤¡¿
ÆóÒµÀàÐÍ£º¾ÏúÉÌ ¡¾ÒÑÈÏÖ¤¡¿
×¢²á×ʽð£ºÈËÃñ±ÒÍò ¡¾ÒÑÈÏÖ¤¡¿
²úÆ·Êý£º8913
²Î¹Û´ÎÊý£º1275917
ÊÖ»úÍøÕ¾£ºhttp://m.yituig.com/c152855/
Æì½¢°æµØÖ·£ºhttp://www.shyqbio.com
ÏêϸÄÚÈÝ
Ö¸±êÃû³Æ£ºÅ£Ó²Ö¬õ£¸¨Ã¸AÈ¥±¥ºÍø(SCD£©ELISA¼ì²âÊÔ¼ÁºÐ
¹æ¸ñ£º48T/ 96T
Æ·ÅÆ£ºÑÐÆôÉúÎï
¼ì²â²¨³¤£º450nm
ËùÐèÑù±¾Ìå»ý£º±£ÏÕÁ¿50ul
ÊÊÓ÷¶Î§£º½ö¹©¿ÆÑпÎÌâÑо¿£¬²»µÃÓÃÓÚÁÙ´²Õï¶Ï¡£
¼ì²âÑù±¾ÖÖÀࣺѪÇ壬Ѫ½¬£¬ÄòÒº£¬×éÖ¯ÔȽ¬£¬Ï¸°ûÉÏÇåÒº£¬ÄÔ¼¹Òº£¬¹àÏ´Òº£¬·à±ãµÈÑù±¾.
Õé¿Æ¹©Ó¦ÊôÖÖÓУºÈË¡¢´óÊó¡¢Ð¡Ê󡢺ï×Ó¡¢ÖíÅ£Ñò¡¢È®¡¢¼¦Ñ¼¶ì¡¢Ö²Îï¡¢Óã¡¢À¥³æELISAÊÔ¼ÁºÐµÈ
ÊÔ¼ÁºÐ±£´æ£º2-8¡æÀä²Í±£´æ£¬±£ÖÊÆÚ6¸öÔ¡£
¶þ¡¢¶ÀÌØÓÅÊÆ
1¡¢ÎÒ˾²úÆ·¾ßÓÐÁéÃô¶È¸ß¡¢ÌØÒìÐÔÇ¿¡¢Öظ´ÐԺõÈÌص㣬Îü¸½ÐÔÄܺÃ,¿Õ°×ÖµµÍ£¬²¢ÊÊÓÃÓÚѪÇåѪ½¬ ×éÖ¯ÔȽ¬µÈ¶àÖÖÑù±¾ÀàÐ͵ļì²â£¬·½·¨¼ò±ã£¬²Ù×÷¼òµ¥¡£
2¡¢²úÆ·±£Ö¤ÖÊÁ¿£¬³ö¿âÖʼì°Ñ¿ØÑϸñ£¬Ë³·á¿ìµÝ£¬ÔËÊäÈ«³Ì¸ú×Ù£¬¹«Ë¾³ÐŵÓÐÈκÎÖÊÁ¿ÎÊÌâ°üÍË»»£¬³ÏОӪ£¬ºÏ×÷¹²Ó®¡£
3¡¢¹«Ë¾³Ðŵ¶©¹ºÎÒ˾ÈκÎÒ»¿îELISAÊÔ¼ÁºÐ£¬ÏíÈ«³Ì¼¼ÊõÖ¸µ¼²¢¿ÉÃâ·Ñ´ú²â¡£È«³ÌÓÐרҵ¼¼ÊõÀÏʦ¸ºÔð´ú²â£¬ÊÕµ½Ñù±¾Ò»°ã5¸ö¹¤×÷ÈÕ³ö½á¹û¡£
4¡¢ÎÒ˾¾ßÓÐÍêÉƵÄÊÛºó·þÎñÌåϵ£¬ÉèÁ¢ÊÛºó·þÎñÖÐÐÄ£¬24Сʱ¼°Ê±ÏìÓ¦£¬ÕæÕý×öµ½ºó¹ËÎÞÓÇ£¬²»¶ÏÍêÉÆ·þÎñÌåϵ¡£¹«Ë¾²úÆ·ÓŻݻ½øÐÐÖУ¬»¶ÓÐÂÀÏ¿Í»§Ç°À´Ñ¡¹º¡£
Èý¡¢ÊÔ¼ÁºÐ×é³É
Ãû³Æ | 96¿×ÅäÖà | 48¿×ÅäÖà | ±¸×¢ |
΢¿×ø±ê°å | 12¿××8Ìõ | 12¿××8Ìõ | ÎÞ |
±ê׼Ʒ | 0.3mL*6¹Ü | 0.3mL*6¹Ü | ÎÞ |
Ñù±¾Ï¡ÊÍÒº | 6mL | 3mL | ÎÞ |
¼ì²â¿¹Ìå-HRP | 10mL | 5mL | ÎÞ |
20×Ï´µÓ»º³åÒº | 25mL | 15mL | °´ËµÃ÷Êé½øÐÐÏ¡ÊÍ |
µ×ÎïA | 6mL | 3mL | ÎÞ |
µ×ÎïB | 6mL | 3mL | ÎÞ |
ÖÕÖ¹Òº | 6mL | 3mL | ÎÞ |
·â°åĤ | 2ÕÅ | 2ÕÅ | ÎÞ |
˵Ã÷Êé | 1·Ý | 1·Ý | ÎÞ |
×Ô·â´ü | 1¸ö | 1¸ö | ÎÞ |
ËÄ¡¢Å£Ó²Ö¬õ£¸¨Ã¸AÈ¥±¥ºÍø(SCD£©ELISA¼ì²âÊÔ¼ÁºÐ²Ù×÷Á÷³Ì
1.´ÓÊÒÎÂƽºâ20minºóµÄÂÁ²´üÖÐÈ¡³öËùÐè°åÌõ£¬Ê£Óà°åÌõÓÃ×Ô·â´üÃÜ·â·Å»Ø4¡æ¡£
2.ÉèÖñê׼Ʒ¿×ºÍÑù±¾¿×£¬±ê׼Ʒ¿×¸÷¼Ó²»Í¬Å¨¶ÈµÄ±ê׼Ʒ50μL£»
3.Ñù±¾¿×ÖмÓÈë´ý²âÑù±¾50μL£»¿Õ°×¿×²»¼Ó¡£
4.³ý¿Õ°×¿×Í⣬±ê׼Ʒ¿×ºÍÑù±¾¿×ÖÐÿ¿×¼ÓÈëÀ±¸ù¹ýÑõ»¯Îïø£¨HRP£©±ê¼ÇµÄ¼ì²â¿¹Ìå100μL£¬Ó÷â°åĤ·âס·´Ó¦¿×£¬37¡æˮԡ¹ø»òºãÎÂÏäÎÂÓý60min¡£
5.ÆúÈ¥ÒºÌ壬ÎüˮֽÉÏÅĸɣ¬Ã¿¿×¼ÓÂúÏ´µÓÒº£¨350μL£©£¬¾²ÖÃ1min£¬Ë¦È¥Ï´µÓÒº£¬ÎüˮֽÉÏÅĸɣ¬Èç´ËÖظ´Ï´°å5´Î£¨Ò²¿ÉÓÃÏ´°å»úÏ´°å£©¡£
6.ÿ¿×¼ÓÈëµ×ÎïA¡¢B¸÷50μL£¬37¡æ±Ü¹â·õÓý15min¡£
7.ÿ¿×¼ÓÈëÖÕÖ¹Òº50μL£¬15minÄÚ£¬ÔÚ450nm²¨³¤´¦²â¶¨¸÷¿×µÄODÖµ¡£
Îå¡¢×¢ÒâÊÂÏî
1£® ÊÔ¼ÁºÐ´ÓÀä²Ø»·¾³ÖÐÈ¡³öÓ¦ÔÚÊÒÎÂƽºâ15-30·ÖÖӺ󷽿ÉʹÓã¬Ã¸±ê°ü±»°å¿ª·âºóÈçδÓÃÍ꣬°åÌõӦװÈëÃÜ·â´üÖб£´æ¡£Å¨Ï´µÓÒº¿ÉÄÜ»áÓнᾧÎö³ö£¬Ï¡ÊÍʱ¿ÉÔÚˮԡÖмÓÎÂÖúÈÜ£¬Ï´µÓʱ²»Ó°Ïì½á¹û¡£
2.¸÷²½¼ÓÑù¾ùӦʹÓüÓÑùÆ÷£¬²¢¾³£Ð£¶ÔÆä׼ȷÐÔ£¬ÒÔ±ÜÃâÊÔÑéÎó²î¡£Ò»´Î¼ÓÑùʱ¼ä¿ØÖÆÔÚ5·ÖÖÓÄÚ£¬Èç±ê±¾ÊýÁ¿¶à£¬ÍƼöʹÓÃÅÅǹ¼ÓÑù¡£
3.Çëÿ´Î²â¶¨µÄͬʱ×ö±ê×¼ÇúÏߣ¬×ö¸´¿×¡£Èç±ê±¾Öдý²âÎïÖʺ¬Á¿¹ý¸ß£¨Ñù±¾ODÖµ´óÓÚ±ê׼Ʒ¿×diÒ»¿×µÄODÖµ£©£¬ÇëÏÈÓÃÑùÆ·Ï¡ÊÍҺϡÊÍÒ»¶¨±¶Êý£¨n±¶£©ºóÔٲⶨ£¬¼ÆËãʱÇë*ºó³ËÒÔ×ÜÏ¡Êͱ¶Êý£¨×n×5£©¡£
4.·â°åĤֻÏÞÒ»´ÎÐÔʹÓã¬ÒÔ±ÜÃâ½»²æÎÛȾ¡£
5.µ×ÎïÇë±Ü¹â±£´æ¡£
6.Ñϸñ°´ÕÕÅ£Ó²Ö¬õ£¸¨Ã¸AÈ¥±¥ºÍø(SCD£©ELISA¼ì²âÊÔ¼ÁºÐ˵Ã÷ÊéµÄ²Ù×÷½øÐУ¬ÊÔÑé½á¹ûÅж¨±ØÐëÒÔø±êÒǶÁÊýΪ׼.
7.ËùÓÐÑùÆ·£¬Ï´µÓÒººÍ¸÷ÖÖ·ÏÆúÎﶼӦ°´´«È¾Îï´¦Àí¡£
8.±¾ÊÔ¼Á²»Í¬ÅúºÅ×é·Ö²»µÃ»ìÓá£
9.²»ÄÜʹÓùýÆÚ²úÆ·¡£
10. ÈçÓëÓ¢ÎÄ˵Ã÷ÊéÓÐÒ죬ÒÔÓ¢ÎÄ˵Ã÷ÊéΪ׼¡£
Áù¡¢¸ü¶àÓÅÊƲúÆ·ÍƼö
´óÊó´Æ¶þ´¼ÊÜÌå(ER£©ELISA¼ì²âÊÔ¼ÁºÐ
Ö²Îï¹ûÌÇ-1,6-¶þÁ×Ëáø(FBPase£©ELISA¼ì²âÊÔ¼ÁºÐ
ÈËÁÁ°±Ëá°±ëÄø(LAP£©ELISA¼ì²âÊÔ¼ÁºÐ
´óÊóĸÇ×DDPͬԴÎï3(Smad3£©ELISA¼ì²âÊÔ¼ÁºÐ
΢ÉúÎﻹÔÐ͸¨Ã¸I(NADH£©ELISA¼ì²âÊÔ¼ÁºÐ
ÈËÓÄÃÅÂݸ˾ú¿¹Ô(HP£©ELISA¼ì²âÊÔ¼ÁºÐ
СÊóάÉúËØD(VD£©ELISA¼ì²âÊÔ¼ÁºÐ
ÈËÑõ»¯µÍÃܶÈÖ¬µ°°×(OxLDL£©ELISA¼ì²âÊÔ¼ÁºÐ
´óÊóapelin 12(AP12£©ELISA¼ì²âÊÔ¼ÁºÐ
ëàÊó¦Ã¸ÉÈÅËØ(IFN-¦Ã£©ELISA¼ì²âÊÔ¼ÁºÐ
Ö²ÎïÏßÁ£ÌåºôÎüÁ´¸´ºÏÎïII(MRCC II£©ELISA¼ì²âÊÔ¼ÁºÐ
ÈË4-ôÇ»ù±½±ûͪËáË«¼ÓÑõø(HPD£©ELISA¼ì²âÊÔ¼ÁºÐ
´óÊó¸Æµ÷Á×Ëáø(CaN£©ELISA¼ì²âÊÔ¼ÁºÐ
Ѽ̹²¼ËÕ²¡¶¾(TV£©ELISA¼ì²âÊÔ¼ÁºÐ