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技术文章
K-12 MG1655大肠杆菌菌株
点击次数：554 发布时间：2020/12/23 18:00:39
产品名称： K-12 MG1655大肠杆菌克隆菌株
产品编号： JZ013
规格： 100μl
储存： -20℃
基因型： F- lambda- ilvG- rfb-50 rph-1(NC_000913)
抗性： 无
培养基： LB
菌株类别： 大肠杆菌
培养条件： 37℃，有氧，LB
质粒转化： 42℃热激
保存方式： 20%甘油，-20℃
基本应用： 用于基因克隆
菌株简介： K-12 MG1655是株K系列的大肠杆菌，经检测无Amp和Kan抗性，可用LB培养基，37℃培养，其代谢类型是异养兼性厌氧型，可用于代谢研究、基因编辑等实验。
The original K-12 wild-type strain from the Stanford collection contained both the F plasmid ａnd phage lambda. MG1655 was constructed by curing that strain using acridine orange ａnd UV.This strain was sequenced by the Blattner laboratory because it approximates wild-type E. coli ａnd "has been maintained as a laboratory strain with minimal genetic manipulation, having only been cured of the temperate bacteriophage lambda ａnd F plasmid by means of ultraviolet light ａnd acridine orange, respectively." (Blattner, et al. 1997). The mutations listed in the genotype are present in most K-12 strains ａnd were probably acquired early in the history of the laboratory strain. A frameshift at the end of rph results in decreased pyrE expression ａnd a mild pyrimidine starvation, such that the strain grows 10 to 15% more slowly in pyrimidine-free medium than in medium containing uracil (Jensen 1993). The ilvG- mutation is a frameshift that knocks out acetohydroxy acid synthase II (Lawther, et al. 1982). The rfb-50 mutation is an IS5 insertion that results in the absence of O-antigen synthesis (Liu ａnd Reeves 1994).
MG1655 was derived ａnd named by Mark Guyer from strain W1485, which was derived in Joshua Lederberg＇s lab from a stab-culture descendant of the original K-12 isolate. This original E. coli strain K-12 was obtained from a stool sample of a diphtheria patient in Palo Alto, CA in 1922 (Bachmann, B., pp. 2460-2488 in Neidhardt et al.1996, Escherichia coli ａnd Salmonella: Cellular ａnd Molecular Biology, ASM Press).MG1655 grows on LB ａnd M9 minimal medium (+ Glucose + 1ug/ml thiamine).
原创作者：上海圻明生物科技有限公司
