企业档案
- 会员类型:免费会员
- 工商认证: 【已认证】
- 最后认证时间:
- 法人:
- 注册号:
- 企业类型:生产商
- 注册资金:人民币万
联系我们
联系人:邓强
热门标签
技术文章
大鼠胶质肉瘤细胞9L/lacZ
点击次数:338 发布时间:2021/1/8 10:52:07
名称大鼠胶质肉瘤细胞9L/lacZ
别称 9L/LacZ
种属 大鼠
年龄(性别) 雄性
组织来源 脑,胶质细胞
生长特性 贴壁细胞
细胞形态 成纤维细胞样
背景描述
The cells constitutively express the lacZ reporter gene product, E. coli derived beta-gal, as revealed o
sections by histochemical stain, and single tumor cells can be identified. Lymphocytes and other resp
cells can be identified by double labeling with antibodies on the same slide. The contrast between sta
and background facilitates image analysis. The beta-gal expression is very stable, but cells may need
cloned after months of growth in culture.
生物安全等 1
生长培养基 DMEM(PM150210)+10%FBS(164210-500)+1%P/S(PB180120)
推荐传代比例 1:4-1:8
推荐换液频率 2~3次/周
冻存条件 冻存液:55% 基础培养基+40%FBS+5%DMSO
温度:液氮
培养条件 气相:空气,95%;CO2,5%
温度:37℃
致瘤性 Yes, forms tumors in the brains of CD Fischer 344 rats
名称大鼠胶质肉瘤细胞9L/lacZ
别称 9L/LacZ
种属 大鼠
年龄(性别) 雄性
组织来源 脑,胶质细胞
生长特性 贴壁细胞
细胞形态 成纤维细胞样
背景描述
The cells constitutively express the lacZ reporter gene product, E. coli derived beta-gal, as revealed o
sections by histochemical stain, and single tumor cells can be identified. Lymphocytes and other resp
cells can be identified by double labeling with antibodies on the same slide. The contrast between sta
and background facilitates image analysis. The beta-gal expression is very stable, but cells may need
cloned after months of growth in culture.
生物安全等 1
生长培养基 DMEM(PM150210)+10%FBS(164210-500)+1%P/S(PB180120)
推荐传代比例 1:4-1:8
推荐换液频率 2~3次/周
冻存条件 冻存液:55% 基础培养基+40%FBS+5%DMSO
温度:液氮
培养条件 气相:空气,95%;CO2,5%
温度:37℃
致瘤性 Yes, forms tumors in the brains of CD Fischer 344 rats
原创作者:上海圻明生物科技有限公司