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描述:XBridge BEH C18 XP Column, 130Å, 2.5 µm, 2.1 mm X 50 mm, 1/pk
eXtended Performance [XP] 2.5 µm色谱柱
waters XP 2.5 µm色谱柱
waters XP 2.5 µm色谱柱 填料性能,提升HPLC分析效率
粒径2.5 µm,提高分离性能,缩短分析时间
填料化学性能,与HPLC柱[XBridge][XSelect CSH/HSS]完全致
尤其适用于UHPLC系统(色谱柱耐压可达9000psig以上)
waters XP 2.5 µm色谱柱 降低UPLC/UHPLC系统运行压力
与<2 µm UPLC柱粒径相比,同等规格2.5 µm色谱柱背压可降低40%
填料化学性能,与ACQUITY UPLC柱[BEH][CSH][HSS]颗粒完全致
2.1 mm和3.0 mm内径规格,耐压达15000 psi,带有eCord可实现无 纸化追踪
waters XP 2.5 µm色谱柱 选择的灵活性
柱规格丰富:14种填料,3种内径(2.1/3.0/4.6 mm),5种柱长 (30/50/75/100/150),根据实验室系统与应用所需随心搭配
[XP] 2.5 µm色谱柱订购信息
1.waters XP 2.5 µm色谱柱
键合相 粒径 规格 部件编号1/包 部件编号3/包
XBridge BEH C18 2.5 μm 2.1 x 30 mm 186006028 176002546
XBridge BEH C18 2.5 μm 2.1 x 50 mm 186006029 176002547
XBridge BEH C18 2.5 μm 2.1 x 75 mm 186006030 176002548
XBridge BEH C18 2.5 μm 2.1 x 100 mm 186006031 176002549
XBridge BEH C18 2.5 μm 2.1 x 150 mm 186006709 176002879
XBridge BEH C18 2.5 μm 3.0 x 30 mm 186006032 176002550
XBridge BEH C18 2.5 μm 3.0 x 50 mm 186006033 176002551
XBridge BEH C18 2.5 μm 3.0 x 75 mm 186006034 176002552
XBridge BEH C18 2.5 μm 3.0 x 100 mm 186006035 176002553
XBridge BEH C18 2.5 μm 3.0 x 150 mm 186006710 176002880
XBridge BEH C18 2.5 μm 4.6 x 30 mm 186006036 -
XBridge BEH C18 2.5 μm 4.6 x 50 mm 186006037 -
XBridge BEH C18 2.5 μm 4.6 x 75 mm 186006038 -
XBridge BEH C18 2.5 μm 4.6 x 100 mm 186006039 -
XBridge BEH C18 2.5 μm 4.6 x 150 mm 186006711 -
XBridge BEH Shield RP18 2.5 μm 2.1 x 30 mm 186006052 176002562
XBridge BEH Shield RP18 2.5 μm 2.1 x 50 mm 186006053 176002563
XBridge BEH Shield RP18 2.5 μm 2.1 x 75 mm 186006054 176002564
XBridge BEH Shield RP18 2.5 μm 2.1 x 100 mm 186006055 176002565
XBridge BEH Shield RP18 2.5 μm 2.1 x 150 mm 186006715 176002883
XBridge BEH Shield RP18 2.5 μm 3.0 x 30 mm 186006056 176002566
XBridge BEH Shield RP18 2.5 μm 3.0 x 50 mm 186006057 176002567
XBridge BEH Shield RP18 2.5 μm 3.0 x 75 mm 186006058 176002568
XBridge BEH Shield RP18 2.5 μm 3.0 x 100 mm 186006059 176002569
XBridge BEH Shield RP18 2.5 μm 3.0 x 150 mm 186006716 176002884
XBridge BEH Shield RP18 2.5 μm 4.6 x 30 mm 186006060 -
XBridge BEH Shield RP18 2.5 μm 4.6 x 50 mm 186006061 -
XBridge BEH Shield RP18 2.5 μm 4.6 x 75 mm 186006062 -
XBridge BEH Shield RP18 2.5 μm 4.6 x 100 mm 186006063 -
XBridge BEH Shield RP18 2.5 μm 4.6 x 150 mm 186006717 -
XBridge BEH C8 2.5 μm 2.1 x 30 mm 186006040 176002554
XBridge BEH C8 2.5 μm 2.1 x 50 mm 186006041 176002555
XBridge BEH C8 2.5 μm 2.1 x 75 mm 186006042 176002556
XBridge BEH C8 2.5 μm 2.1 x 100 mm 186006043 176002557
XBridge BEH C8 2.5 μm 2.1 x 150 mm 186006712 176002881
XBridge BEH C8 2.5 μm 3.0 x 30 mm 186006044 176002558
XBridge BEH C8 2.5 μm 3.0 x 50 mm 186006045 176002559
XBridge BEH C8 2.5 μm 3.0 x 75 mm 186006046 176002560
XBridge BEH C8 2.5 μm 3.0 x 100 mm 186006047 176002561
XBridge BEH C8 2.5 μm 3.0 x 150 mm 186006713 176002882
XBridge BEH C8 2.5 μm 4.6 x 30 mm 186006048 -
XBridge BEH C8 2.5 μm 4.6 x 50 mm 186006049 -
XBridge BEH C8 2.5 μm 4.6 x 75 mm 186006050 -
XBridge BEH C8 2.5 μm 4.6 x 100 mm 186006051 -
XBridge BEH C8 2.5 μm 4.6 x 150 mm 186006714 -
XBridge BEH Phenyl 2.5 μm 2.1 x 30 mm 186006064 176002570
XBridge BEH Phenyl 2.5 μm 2.1 x 50 mm 186006065 176002571
XBridge BEH Phenyl 2.5 μm 2.1 x 75 mm 186006066 176002572
XBridge BEH Phenyl 2.5 μm 2.1 x 100 mm 186006067 176002573
XBridge BEH Phenyl 2.5 μm 2.1 x 150 mm 186006718 176002885
XBridge BEH Phenyl 2.5 μm 3.0 x 30 mm 186006068 176002574
XBridge BEH Phenyl 2.5 μm 3.0 x 50 mm 186006069 176002575
XBridge BEH Phenyl 2.5 μm 3.0 x 75 mm 186006070 176002576
XBridge BEH Phenyl 2.5 μm 3.0 x 100 mm 186006071 176002577
XBridge BEH Phenyl 2.5 μm 3.0 x 150 mm 186006719 176002886
XBridge BEH Phenyl 2.5 μm 4.6 x 30 mm 186006072 -
XBridge BEH Phenyl 2.5 μm 4.6 x 50 mm 186006073 -
XBridge BEH Phenyl 2.5 μm 4.6 x 75 mm 186006074 -
XBridge BEH Phenyl 2.5 μm 4.6 x 100 mm 186006075 -
XBridge BEH Phenyl 2.5 μm 4.6 x 150 mm 186006720 -
XBridge BEH HILIC 2.5 μm 2.1 x 30 mm 186006076 176002578
XBridge BEH HILIC 2.5 μm 2.1 x 50 mm 186006077 176002579
XBridge BEH HILIC 2.5 μm 2.1 x 75 mm 186006078 176002580
XBridge BEH HILIC 2.5 μm 2.1 x 100 mm 186006079 176002581
XBridge BEH HILIC 2.5 μm 2.1 x 150 mm 186006721 176002887
XBridge BEH HILIC 2.5 μm 3.0 x 30 mm 186006080 176002582
XBridge BEH HILIC 2.5 μm 3.0 x 50 mm 186006081 176002583
XBridge BEH HILIC 2.5 μm 3.0 x 75 mm 186006082 176002584
XBridge BEH HILIC 2.5 μm 3.0 x 100 mm 186006083 176002585
XBridge BEH HILIC 2.5 μm 3.0 x 150 mm 186006722 176002888
XBridge BEH HILIC 2.5 μm 4.6 x 30 mm 186006084 -
XBridge BEH HILIC 2.5 μm 4.6 x 50 mm 186006085 -
XBridge BEH HILIC 2.5 μm 4.6 x 75 mm 186006086 -
XBridge BEH HILIC 2.5 μm 4.6 x 100 mm 186006087 -
XBridge BEH HILIC 2.5 μm 4.6 x 150 mm 186006723 -
XBridge BEH Amide 2.5 μm 2.1 x 30 mm 186006088 176002586
XBridge BEH Amide 2.5 μm 2.1 x 50 mm 186006089 176002587
XBridge BEH Amide 2.5 μm 2.1 x 75 mm 186006090 176002588
XBridge BEH Amide 2.5 μm 2.1 x 100 mm 186006091 176002589
XBridge BEH Amide 2.5 μm 2.1 x 150 mm 186006724 176002889
XBridge BEH Amide 2.5 μm 3.0 x 30 mm 186006092 176002590
XBridge BEH Amide 2.5 μm 3.0 x 50 mm 186006093 176002591
XBridge BEH Amide 2.5 μm 3.0 x 75 mm 186006094 176002592
XBridge BEH Amide 2.5 μm 3.0 x 100 mm 186006095 176002593
XBridge BEH Amide 2.5 μm 3.0 x 150 mm 186006725 176002890
XBridge BEH Amide 2.5 μm 4.6 x 30 mm 186006096 -
XBridge BEH Amide 2.5 μm 4.6 x 50 mm 186006097 -
XBridge BEH Amide 2.5 μm 4.6 x 75 mm 186006098 -
XBridge BEH Amide 2.5 μm 4.6 x 100 mm 186006099 -
XBridge BEH Amide 2.5 μm 4.6 x 150 mm 186006726 -
2.VanGuard? Pre-Column 3 Pack (保护柱)
键合相 粒径 规格 部件编号3/包
XBridge BEH C18 2.5 μm 2.1 x 5 mm 186006291
XBridge BEH Shield RP18 2.5 μm 2.1 x 5 mm 186006293
XBridge BEH C8 2.5 μm 2.1 x 5 mm 186006292
XBridge BEH Phenyl 2.5 μm 2.1 x 5 mm 186006294
XBridge BEH HILIC 2.5 μm 2.1 x 5 mm 186006295
XBridge BEH Amide 2.5 μm 2.1 x 5 mm 186006296
XSelect CSH C18 2.5 μm 2.1 x 5 mm 186006297
XSelect CSH Fluoro-Phenyl 2.5 μm 2.1 x 5 mm 186006298
XSelect CSH Phenyl-Hexyl 2.5 μm 2.1 x 5 mm 186006299
XSelect HSS T3 2.5 μm 2.1 x 5 mm 186006301
XSelect HSS C18 2.5 μm 2.1 x 5 mm 186006300
XSelect HSS C18 SB 2.5 μm 2.1 x 5 mm 186006302
XSelect HSS Cyano 2.5 μm 2.1 x 5 mm 186006304
XSelect HSS PFP 2.5 μm 2.1 x 5 mm 186006303
3.XSelect CSH XP 2.5 μm 色谱柱
键合相 粒径 规格 部件编号1/包 部件编号3/包
XSelect CSH C18 2.5 μm 2.1 x 30 mm 186006100 176002594
XSelect CSH C18 2.5 μm 2.1 x 50 mm 186006101 176002595
XSelect CSH C18 2.5 μm 2.1 x 75 mm 186006102 176002596
XSelect CSH C18 2.5 μm 2.1 x 100 mm 186006103 176002597
XSelect CSH C18 2.5 μm 2.1 x 150 mm 186006727 176002891
XSelect CSH C18 2.5 μm 3.0 x 30 mm 186006104 176002598
XSelect CSH C18 2.5 μm 3.0 x 50 mm 186006105 176002599
XSelect CSH C18 2.5 μm 3.0 x 75 mm 186006106 176002600
XSelect CSH C18 2.5 μm 3.0 x 100 mm 186006107 176002601
XSelect CSH C18 2.5 μm 3.0 x 150 mm 186006728 176002892
XSelect CSH C18 2.5 μm 4.6 x 30 mm 186006108 -
XSelect CSH C18 2.5 μm 4.6 x 50 mm 186006109 -
XSelect CSH C18 2.5 μm 4.6 x 75 mm 186006110 -
XSelect CSH C18 2.5 μm 4.6 x 100 mm 186006111 -
XSelect CSH C18 2.5 μm 4.6 x 150 mm 186006729 -
XSelect CSH Fluoro-Phenyl 2.5 μm 2.1 x 30 mm 186006112 176002602
XSelect CSH Fluoro-Phenyl 2.5 μm 2.1 x 50 mm 186006113 176002603
XSelect CSH Fluoro-Phenyl 2.5 μm 2.1 x 75 mm 186006114 176002604
XSelect CSH Fluoro-Phenyl 2.5 μm 2.1 x 100 mm 186006115 176002605
XSelect CSH Fluoro-Phenyl 2.5 μm 2.1 x 150 mm 186006730 176002893
XSelect CSH Fluoro-Phenyl 2.5 μm 3.0 x 30 mm 186006116 176002606
XSelect CSH Fluoro-Phenyl 2.5 μm 3.0 x 50 mm 186006117 176002607
XSelect CSH Fluoro-Phenyl 2.5 μm 3.0 x 75 mm 186006118 176002608
XSelect CSH Fluoro-Phenyl 2.5 μm 3.0 x 100 mm 186006119 176002609
XSelect CSH Fluoro-Phenyl 2.5 μm 3.0 x 150 mm 186006731 176002894
XSelect CSH Fluoro-Phenyl 2.5 μm 4.6 x 30 mm 186006120 -
XSelect CSH Fluoro-Phenyl 2.5 μm 4.6 x 50 mm 186006121 -
XSelect CSH Fluoro-Phenyl 2.5 μm 4.6 x 75 mm 186006122 -
XSelect CSH Fluoro-Phenyl 2.5 μm 4.6 x 100 mm 186006123 -
XSelect CSH Fluoro-Phenyl 2.5 μm 4.6 x 150 mm 186006732 -
XSelect CSH Phenyl-Hexyl 2.5 μm 2.1 x 30 mm 186006124 176002610
XSelect CSH Phenyl-Hexyl 2.5 μm 2.1 x 50 mm 186006125 176002611
XSelect CSH Phenyl-Hexyl 2.5 μm 2.1 x 75 mm 186006126 176002612
XSelect CSH Phenyl-Hexyl 2.5 μm 2.1 x 100 mm 186006127 176002613
XSelect CSH Phenyl-Hexyl 2.5 μm 2.1 x 150 mm 186006733 176002895
XSelect CSH Phenyl-Hexyl 2.5 μm 3.0 x 30 mm 186006128 176002614
XSelect CSH Phenyl-Hexyl 2.5 μm 3.0 x 50 mm 186006129 176002615
XSelect CSH Phenyl-Hexyl 2.5 μm 3.0 x 75 mm 186006130 176002616
XSelect CSH Phenyl-Hexyl 2.5 μm 3.0 x 100 mm 186006131 176002617
XSelect CSH Phenyl-Hexyl 2.5 μm 3.0 x 150 mm 186006734 176002896
XSelect CSH Phenyl-Hexyl 2.5 μm 4.6 x 30 mm 186006132 -
XSelect CSH Phenyl-Hexyl 2.5 μm 4.6 x 50 mm 186006133 -
XSelect CSH Phenyl-Hexyl 2.5 μm 4.6 x 75 mm 186006134 -
XSelect CSH Phenyl-Hexyl 2.5 μm 4.6 x 100 mm 186006135 -
XSelect CSH Phenyl-Hexyl 2.5 μm 4.6 x 150 mm 186006735 -
4.XSelect HSS XP 2.5 μm 色谱柱
键合相 粒径 规格 部件编号1/包 部件编号3/包
XSelect HSS T3 2.5 μm 2.1 x 30 mm 186006148 176002626
XSelect HSS T3 2.5 μm 2.1 x 50 mm 186006149 176002627
XSelect HSS T3 2.5 μm 2.1 x 75 mm 186006150 176002628
XSelect HSS T3 2.5 μm 2.1 x 100 mm 186006151 176002629
XSelect HSS T3 2.5 μm 2.1 x 150 mm 186006739 176002899
XSelect HSS T3 2.5 μm 3.0 x 30 mm 186006152 176002630
XSelect HSS T3 2.5 μm 3.0 x 50 mm 186006153 176002631
XSelect HSS T3 2.5 μm 3.0 x 75 mm 186006154 176002632
XSelect HSS T3 2.5 μm 3.0 x 100 mm 186006155 176002633
XSelect HSS T3 2.5 μm 3.0 x 150 mm 186006740 176002900
XSelect HSS T3 2.5 μm 4.6 x 30 mm 186006156 -
XSelect HSS T3 2.5 μm 4.6 x 50 mm 186006157 -
XSelect HSS T3 2.5 μm 4.6 x 75 mm 186006158 -
XSelect HSS T3 2.5 μm 4.6 x 100 mm 186006159 -
XSelect HSS T3 2.5 μm 4.6 x 150 mm 186006741 -
XSelect HSS C18 2.5 μm 2.1 x 30 mm 186006136 176002618
XSelect HSS C18 2.5 μm 2.1 x 50 mm 186006137 176002619
XSelect HSS C18 2.5 μm 2.1 x 75 mm 186006138 176002620
XSelect HSS C18 2.5 μm 2.1 x 100 mm 186006139 176002621
XSelect HSS C18 2.5 μm 2.1 x 150 mm 186006736 176002897
XSelect HSS C18 2.5 μm 3.0 x 30 mm 186006140 176002622
XSelect HSS C18 2.5 μm 3.0 x 50 mm 186006141 176002623
XSelect HSS C18 2.5 μm 3.0 x 75 mm 186006142 176002624
XSelect HSS C18 2.5 μm 3.0 x 100 mm 186006143 176002625
XSelect HSS C18 2.5 μm 3.0 x 150 mm 186006737 176002898
XSelect HSS C18 2.5 μm 4.6 x 30 mm 186006144 -
XSelect HSS C18 2.5 μm 4.6 x 50 mm 186006145 -
XSelect HSS C18 2.5 μm 4.6 x 75 mm 186006146 -
XSelect HSS C18 2.5 μm 4.6 x 100 mm 186006147 -
XSelect HSS C18 2.5 μm 4.6 x 150 mm 186006738 -
XSelect HSS C18 SB 2.5 μm 2.1 x 30 mm 186006160 176002634
XSelect HSS C18 SB 2.5 μm 2.1 x 50 mm 186006161 176002635
XSelect HSS C18 SB 2.5 μm 2.1 x 75 mm 186006162 176002636
XSelect HSS C18 SB 2.5 μm 2.1 x 100 mm 186006163 176002637
XSelect HSS C18 SB 2.5 μm 2.1 x 150 mm 186006742 176002901
XSelect HSS C18 SB 2.5 μm 3.0 x 30 mm 186006164 176002638
XSelect HSS C18 SB 2.5 μm 3.0 x 50 mm 186006165 176002639
XSelect HSS C18 SB 2.5 μm 3.0 x 75 mm 186006166 176002640
XSelect HSS C18 SB 2.5 μm 3.0 x 100 mm 186006167 176002641
XSelect HSS C18 SB 2.5 μm 3.0 x 150 mm 186006743 176002902
XSelect HSS C18 SB 2.5 μm 4.6 x 30 mm 186006168 -
XSelect HSS C18 SB 2.5 μm 4.6 x 50 mm 186006169 -
XSelect HSS C18 SB 2.5 μm 4.6 x 75 mm 186006170 -
XSelect HSS C18 SB 2.5 μm 4.6 x 100 mm 186006171 -
XSelect HSS C18 SB 2.5 μm 4.6 x 150 mm 186006744 -
XSelect HSS Cyano 2.5 μm 2.1 x 30 mm 186006184 176002650
XSelect HSS Cyano 2.5 μm 2.1 x 50 mm 186006185 176002651
XSelect HSS Cyano 2.5 μm 2.1 x 75 mm 186006186 176002652
XSelect HSS Cyano 2.5 μm 2.1 x 100 mm 186006187 176002653
XSelect HSS Cyano 2.5 μm 2.1 x 150 mm 186006748 176002905
XSelect HSS Cyano 2.5 μm 3.0 x 30 mm 186006188 176002654
XSelect HSS Cyano 2.5 μm 3.0 x 50 mm 186006189 176002655
XSelect HSS Cyano 2.5 μm 3.0 x 75 mm 186006190 176002656
XSelect HSS Cyano 2.5 μm 3.0 x 100 mm 186006191 176002657
XSelect HSS Cyano 2.5 μm 3.0 x 150 mm 186006749 176002906
XSelect HSS Cyano 2.5 μm 4.6 x 30 mm 186006192 -
XSelect HSS Cyano 2.5 μm 4.6 x 50 mm 186006193 -
XSelect HSS Cyano 2.5 μm 4.6 x 75 mm 186006194 -
XSelect HSS Cyano 2.5 μm 4.6 x 100 mm 186006195 -
XSelect HSS Cyano 2.5 μm 4.6 x 150 mm 186006750 -
XSelect HSS PFP 2.5 μm 2.1 x 30 mm 186006172 176002642
XSelect HSS PFP 2.5 μm 2.1 x 50 mm 186006173 176002643
XSelect HSS PFP 2.5 μm 2.1 x 75 mm 186006174 176002644
XSelect HSS PFP 2.5 μm 2.1 x 100 mm 186006175 176002645
XSelect HSS PFP 2.5 μm 2.1 x 150 mm 186006745 176002903
XSelect HSS PFP 2.5 μm 3.0 x 30 mm 186006176 176002646
XSelect HSS PFP 2.5 μm 3.0 x 50 mm 186006177 176002647
XSelect HSS PFP 2.5 μm 3.0 x 75 mm 186006178 176002648
XSelect HSS PFP 2.5 μm 3.0 x 100 mm 186006179 176002649
XSelect HSS PFP 2.5 μm 3.0 x 150 mm 186006746 176002904
XSelect HSS PFP 2.5 μm 4.6 x 30 mm 186006180 -
XSelect HSS PFP 2.5 μm 4.6 x 50 mm 186006181 -
XSelect HSS PFP 2.5 μm 4.6 x 75 mm 186006182 -
XSelect HSS PFP 2.5 μm 4.6 x 100 mm 186006183 -
XSelect HSS PFP 2.5 μm 4.6 x 150 mm 186006747 -
5.XBridge/XSelect Columns 方法验证套件 [MVK]*
键合相 粒径 色谱柱长度 部件编号2.1 mm ID 部件编号3.0 mm ID 部件编号4.6 mm ID
XBridge BEH C18 2.5 μm 50 mm 186006197 186006199 186006201
XBridge BEH C18 2.5 μm 100 mm 186006198 186006200 186006202
XBridge BEH C18 2.5 μm 150 mm 186006757 186006758 186006759
XBridge BEH Shield
RP18
2.5 μm 50 mm 186006209 186006211 186006213
XBridge BEH Shield
RP18
2.5 μm 100 mm 186006210 186006212 186006214
XBridge BEH Shield
RP18
2.5 μm 150 mm 186006763 186006774 186006775
XBridge BEH C8 2.5 μm 50 mm 186006203 186006205 186006207
XBridge BEH C8 2.5 μm 100 mm 186006204 186006206 186006208
XBridge BEH C8 2.5 μm 150 mm 186006760 186006761 186006762
XBridge BEH Phenyl 2.5 μm 50 mm 186006215 186006217 186006219
XBridge BEH Phenyl 2.5 μm 100 mm 186006216 186006218 186006220
XBridge BEH Phenyl 2.5 μm 150 mm 186006776 186006777 186006778
XBridge BEH HILIC 2.5 μm 50 mm 186006221 186006223 186006225
XBridge BEH HILIC 2.5 μm 100 mm 186006222 186006224 186006226
XBridge BEH HILIC 2.5 μm 150 mm 186006779 186006780 186006781
XBridge BEH Amide 2.5 μm 50 mm 186006227 186006229 186006231
XBridge BEH Amide 2.5 μm 100 mm 186006228 186006230 186006232
XBridge BEH Amide 2.5 μm 150 mm 186006782 186006783 186006784
XSelect CSH C18 2.5 μm 50 mm 186006233 186006235 186006237
XSelect CSH C18 2.5 μm 100 mm 186006234 186006236 186006238
XSelect CSH C18 2.5 μm 150 mm 186006785 186006786 186006787
XSelect CSH Fluoro-
Phenyl
2.5 μm 50 mm 186006239 186006241 186006243
XSelect CSH Fluoro-
Phenyl
2.5 μm 100 mm 186006240 186006242 186006244
XSelect CSH Fluoro-
Phenyl
2.5 μm 150 mm 186006788 186006789 186006790
XSelect CSH Phenyl-
Hexyl
2.5 μm 50 mm 186006245 186006247 186006249
XSelect CSH Phenyl-
Hexyl
2.5 μm 100 mm 186006246 186006248 186006250
XSelect CSH Phenyl-
Hexyl
2.5 μm 150 mm 186006791 186006792 186006793
XSelect HSS T3 2.5 μm 50 mm 186006257 186006259 186006261
XSelect HSS T3 2.5 μm 100 mm 186006258 186006260 186006262
XSelect HSS T3 2.5 μm 150 mm 186006797 186006798 186006799
XSelect HSS C18 2.5 μm 50 mm 186006251 186006253 186006255
XSelect HSS C18 2.5 μm 100 mm 186006252 186006254 186006256
XSelect HSS C18 2.5 μm 150 mm 186006794 186006795 186006796
XSelect HSS C18 SB 2.5 μm 50 mm 186006263 186006265 186006267
XSelect HSS C18 SB 2.5 μm 100 mm 186006264 186006266 186006268
XSelect HSS C18 SB 2.5 μm 150 mm 186006800 186006801 186006802
XSelect HSS Cyano 2.5 μm 50 mm 186006275 186006277 186006279
XSelect HSS Cyano 2.5 μm 100 mm 186006276 186006278 186006280
XSelect HSS Cyano 2.5 μm 150 mm 186006806 186006807 186006808
XSelect HSS PFP 2.5 μm 50 mm 186006815 186006817 186006273
XSelect HSS PFP 2.5 μm 100 mm 186006816 186006818 186006274
XSelect HSS PFP 2.5 μm 150 mm 186006803 186006804 186006805
*每组套件包括用3支不同批次填料的色谱柱。
Waters常规反相色谱柱安装及使用维护指南
何谓常规反相色谱柱?
在硅胶或杂化基质颗粒上键合了如C18、C8、C4或苯基等键合相的色谱填料所装填的色谱柱,按反相色谱条件进行操作和分离。反相是液相色谱中常使用的分离模式,而C18柱是常使用的反相色谱柱之。
新色谱柱开启和安装的推荐步骤:
- 使用新鲜洁净的水与乙腈。冲洗系统,确保系统干净,不含任何缓冲盐和污染物。
- 取用色谱柱时避免磕碰掉落。
- 将色谱柱入口端连接到系统上,柱出口端先不要连接,色谱柱身上有箭头标明正确流向。
- 在0.1 mL/min流速条件下用纯乙腈润洗色谱柱,然后在2分钟内将流速升至0.5 mL/min。
- 当溶剂均匀的从柱出口端流出,停流速,将色谱柱出口端接到系统检测器上(这样可以避免气泡进入检测系统,并且可快速达到基线平衡)。
- 重启流速,按照步骤4的方法逐渐提高流速,至常规分析时所使用的流速。
- 参考柱效测试报告中的方法,使用该流动相条件平衡色谱柱,通常需要使用5-10倍柱体积的流动相,直至压力与基线稳定。
- 测试柱效。如果没有柱效测试报告中的分析物,请联系沃特世化学消耗品部门寻求支持,使用合适的浓度与进样量。柱效结果略低于柱效测试报告属正常情况。如结果明显偏低、峰形拖尾,提示色谱柱和/或所使用的液相系统处于非理想状态,需要进行故障排查。
柱清洗与再生的推荐步骤:
压力升高,色谱柱峰形发生变化如出现严重拖尾、肩峰甚至峰分岔,分离度降低,这些都强烈提示色谱柱受到污染。可采用以下步骤处理:
- 如系统接有在线过滤器和保护柱,先排查在线过滤器与保护柱,进行更新替换。日常操作中,当压力升高10%或柱效降低10%时,就应考虑更换在线过滤器和/或保护柱。
- 使用高比例的有机溶剂进行冲洗(请注意避免盐析出,通常可使用梯度升高法,然后维持在高比例甚至100%有机溶剂条件下)。此操作通常可洗去大部分污染物。
- 如有机溶剂清洗不能解决问题,可采用如下方法进行色谱柱的清洗和再生。根据样品性质以及你所了解的污染物性质选择清洗方法,用20倍柱体积(即,对于4.6x250 mm柱,相当于80 mL)的HPLC溶剂清洗色谱柱,将流动相温度升至35-55˚C可提高清洗效率。(注:该说明摘选自waters产品目录2017-2018版本81页)
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