产品展示
人血管内皮细胞生长因子165（VEGF165）elisa试剂盒方法学
点击次数：14发布时间：2021/9/22 15:06:38
更新日期：2024/9/30 9:38:34
所 在 地：中国大陆
产品型号：
优质供应
详细内容
人血管内皮细胞生长因子165（VEGF165）elisa试剂盒
厦门仑昌硕生物科技有限公司 本公司集研发、生产、销售、服务及品牌代理为体的生物技术公司，目可提供各种抗体、免疫学相关的检测试剂盒、胎牛血清、动物抗血清等高品质产品及服务，涵盖分子生物学、细胞生物学、免疫学等生命科学域。价格实惠，质量有保证，请您放心选购，欢迎来电咨询！
产品资料
Aim To study the injury effect of lipoprotein (a) [Lp(a)] on biological function of mouse bone marrow-derived endothelial progenitor cells (EPC). Methods EPC were divided into control groups ａnd five treatment groups with different concentrations (1, 10, 100, 300 ａnd 600 mg/L) of Lp(a). EPC were isolated by adherence culture ａnd identified by binding of UEA-1, ａnd uptake of acetylated low density lipoprotein (ac-LDL). The abilities of survival, migration ａnd adherence were detected by the MTT, Transwell ａnd gelatin adherence assays respectively. The total length of the tube structures in each photograph was measured using Adobe Photoshop software 7.0. Results Lp(a) dose-dependently decreased the surival rate of EPC, 100 mg/L Lp(a) reduced it significantly ａnd the maximum injury concentration was 300 mg/L. A significantly reduced migratory rate of EPC could be seen after treatment with 1 mg/L Lp(a), the ｃount of the migratory cells was less than 1/6 of the control, so 1/9 for 10 mg/L Lp(a) ａnd 1/14 for 300 mg/L Lp(a). EPC treated with Lp(a) showed a dose-dependent decrease of adhesion to gelatin. 1 mg/L Lp(a) markedly decreased the number of adhesive cells (145.2±8.4/every field vs 115.2±12.6/ every field, P<0.05, n5). When EPC were exposed to 10 or 100 mg/L Lp(a), the capability of adhesion had further descended. When at 300 mg/L, it was remarkably descent to 1/16 of control. Treatment with 10 mg/L Lp(a) impaired the ability of EPC to form tube structures. EPC cultivanted with 100 mg/L had presented decreased tube formation ａnd was less than 1/4 of control (36.34±1.54 mm/field vs 8.76±0.62 mm/field, P<0.001, n5), ａnd when incubated at 300 mg/L of Lp(a), the integrity tube structure was severely disrupted.
样品的处理及保存方法
人血管内皮细胞生长因子165（VEGF165）elisa试剂盒
1、 血清－－－－-操作过程中避免任何细胞刺激。使用不含热原和内毒素的试管。收集血液后，1000×g离心10分钟将血清和红细胞迅速小心地分离。
2、 血浆－－－－-EDTA、柠檬酸盐、肝素血浆可用于检测。1000×g离心30分钟去除颗粒。
3、 细胞上清液－－-1000×g离心10分钟除颗粒和聚合物。
4、 组织匀浆－－－－-将组织加入适量生理盐水捣碎。1000×g离心10分钟，取上清液
5、 保存－－－－－－如果样品不立即使用，应将其分成小部分-70 ℃保存，避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒，检测先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。
公司介绍
人血管内皮细胞生长因子165（VEGF165）elisa试剂盒
厦门仑昌硕生物科技有限公司 本公司集研发、生产、销售、服务及品牌代理为体的生物技术公司，目可提供各种抗体、免疫学相关的检测试剂盒、胎牛血清、动物抗血清等高品质产品及服务，涵盖分子生物学、细胞生物学、免疫学等生命科学域。公司非常注重企业信息化的建设及企业平台的建设，我们内部采用了的信息处理平台，保证客户的需求可以准确、高效处理。公司成立之初，立足外贸，和部分欧美及亚非国家的客户建立了良好的合作，随着国内生物域的蓬勃发展，逐步把重心转移到国内来，为每位科研人员献上高品质的产品及服务是我们的使命。
AMOY LUNCHANGSHUO BIOTECH CO.,LTD.
