产品展示
人转化受体电位阳离子通道亚家族C成员6（TRPC6）elisa试剂盒elisa操作的仪器
点击次数：10发布时间：2021/9/23 11:18:54
更新日期：2024/9/30 9:38:48
所 在 地：中国大陆
产品型号：
优质供应
详细内容
人转化受体电位阳离子通道亚家族C成员6（TRPC6）elisa试剂盒
厦门仑昌硕生物科技有限公司 本公司集研发、生产、销售、服务及品牌代理为体的生物技术公司，目可提供各种抗体、免疫学相关的检测试剂盒、胎牛血清、动物抗血清等高品质产品及服务，涵盖分子生物学、细胞生物学、免疫学等生命科学域。价格实惠，质量有保证，请您放心选购，欢迎来电咨询！
Objective: To study the effect of RA on directional differentiation of cord blood stem cells into neuron-like cells, ａnd to explore the conditions of differentiation of cord blood stem cells into neuron-like cells, so as to provide experimental basis for the study of cord blood stem cells transplantation to repair spinal cord injury. Methods: Mononuclear cells (MNCs) were aseptically separated from cord blood by density gradient centrifugation ａnd cultured in DMEM medium containing 20% fetal bovine serum, ａnd then expanded ａnd passaged under the same conditions. Then, five geneRAtions of umbilical cord blood MSCs expanded in vitro were inoculated into a 6-hole culture plate, ａnd 10ng/ml bFGF was added into the culture medium, ａnd pre-induction was carried out at 37℃ ａnd 5% CO2 for 24h. then, the pre-induction liquid was sucked off, ａnd PBS was washed for three times, ａnd then H-DMEM containing 20%FBS ａnd 20g/L DMSO were added. on this basis, ra with different concentrations was added as inducer for induction. According to different concentrations of inducers ａnd different groups of induction time, the morphological ａnd histological changes of cells were observed by static culture. After induction for 24h,48h ａnd 72h, the cells were taken for immunohistochemical detection of neuron enolase (NSE), neurofilament protein (NF-M) ａnd glial fibrillary acidic protein (GFAP), ａnd then 10 non-overlapping fields (×100) were randomly selected under light microscope to calculate the proportion of positive cells to the total number of cells. )+/-s)%, to explore the effect of RA on the differentiation of umbilical cord blood stem cells into neuron-like cells at different time points. then, after induction for 72 hours, the cells in each group containing 1 μ mol/l, 3 μ mol/l ａnd 5 μ mol/l ra were taken to detect the expression of NSE, nf-m ａnd GFAP by immunohistochemistry. under light microscope, 10 non-overlapping fields (×100) were randomly selected, ａnd the total number of positive cells was calculated.
样品的处理及保存方法
人转化受体电位阳离子通道亚家族C成员6（TRPC6）elisa试剂盒
1、 血清－－－－-操作过程中避免任何细胞刺激。使用不含热原和内毒素的试管。收集血液后，1000×g离心10分钟将血清和红细胞迅速小心地分离。
2、 血浆－－－－-EDTA、柠檬酸盐、肝素血浆可用于检测。1000×g离心30分钟去除颗粒。
3、 细胞上清液－－-1000×g离心10分钟除颗粒和聚合物。
4、 组织匀浆－－－－-将组织加入适量生理盐水捣碎。1000×g离心10分钟，取上清液
5、 保存－－－－－－如果样品不立即使用，应将其分成小部分-70 ℃保存，避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒，检测先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。
公司介绍
人转化受体电位阳离子通道亚家族C成员6（TRPC6）elisa试剂盒
厦门仑昌硕生物科技有限公司 本公司集研发、生产、销售、服务及品牌代理为体的生物技术公司，目可提供各种抗体、免疫学相关的检测试剂盒、胎牛血清、动物抗血清等高品质产品及服务，涵盖分子生物学、细胞生物学、免疫学等生命科学域。公司非常注重企业信息化的建设及企业平台的建设，我们内部采用了的信息处理平台，保证客户的需求可以准确、高效处理。公司成立之初，立足外贸，和部分欧美及亚非国家的客户建立了良好的合作，随着国内生物域的蓬勃发展，逐步把重心转移到国内来，为每位科研人员献上高品质的产品及服务是我们的使命。
AMOY LUNCHANGSHUO BIOTECH CO.,LTD.
