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产品简介 人液泡型H+-ATPase（V-ATPase）elisa试剂盒
厦门仑昌硕生物科技有限公司 本公司集研发、生产、销售、服务及品牌代理为体的生物技术公司，目可提供各种抗体、免疫学相关的检测试剂盒、胎牛血清、动物抗血清等高品质产品及服务，涵盖分子生物学、细胞生物学、免疫学等生命科学域。价格实惠，质量有保证，请您放心选购，欢迎来电咨询！
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人液泡型H+-ATPase（V-ATPase）elisa试剂盒
Objective To observe the role of receptor tyrosine kinase (Axl) in liver cancer cell metastasis ａnd its effect on phosphoinositide 3 kinase (PI3K)/ protein kinase B(Akt)-p21 activation kinase -1(PAK1). The regulation of signal pathway. methods liver cancer cell lines MHCC97-H ａnd MHCC97-L were cultured in vitro. the protein expression level of Axl in liver cancer cell lines MHCC97-H ａnd MHCC97-L was studied by Western blot. the expression of Axl was inhibited by transfection of short hairpin RNA(shRNA). The regulation mechanism of Axl on PI3 K/Akt-PAK1 signaling pathway was explored, ａnd the relationship between Axl protein expression ａnd invasion ａnd metastasis of hepatoma cells was analyzed. results compared with MHCC97-L cell line, Axl had higher expression level in MHCC97-H cell line. the down-regulation of Axl expression in MHCC97-H cell reduced the phosphorylation level of PI3K, Akt ａnd pak1 signaling pathway molecules, ａnd significantly inhibited the phosphorylation of hepatoma cells. The result of invasion experiment showed that the number of cells passing through matrix glue in AxlRNA (siRNA transfection group was (23 3), which was significantly lower than that in quality control group (37 5) ａnd normal cell MHCC97-H group (38 5) (P<0.05). Invasive experiment showed that the number of cells passing through the matrix glue in Akt-siRNA transfection group ａnd PI3K specific inhibitor LY294002 inhibition group was (23 3) ａnd (18 3), which was significantly lower than that in quality control group (37 5) ａnd dimethyl sulfoxide (DMSO) group (38 5) (P<0.05).
样品的处理及保存方法人液泡型H+-ATPase（V-ATPase）elisa试剂盒
1、 血清－－－－-操作过程中避免任何细胞刺激。使用不含热原和内毒素的试管。收集血液后，1000×g离心10分钟将血清和红细胞迅速小心地分离。
2、 血浆－－－－-EDTA、柠檬酸盐、肝素血浆可用于检测。1000×g离心30分钟去除颗粒。
3、 细胞上清液－－-1000×g离心10分钟除颗粒和聚合物。
4、 组织匀浆－－－－-将组织加入适量生理盐水捣碎。1000×g离心10分钟，取上清液
5、 保存－－－－－－如果样品不立即使用，应将其分成小部分-70 ℃保存，避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒，检测先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。
公司介绍人液泡型H+-ATPase（V-ATPase）elisa试剂盒
厦门仑昌硕生物科技有限公司 本公司集研发、生产、销售、服务及品牌代理为体的生物技术公司，目可提供各种抗体、免疫学相关的检测试剂盒、胎牛血清、动物抗血清等高品质产品及服务，涵盖分子生物学、细胞生物学、免疫学等生命科学域。公司非常注重企业信息化的建设及企业平台的建设，我们内部采用了的信息处理平台，保证客户的需求可以准确、高效处理。公司成立之初，立足外贸，和部分欧美及亚非国家的客户建立了良好的合作，随着国内生物域的蓬勃发展，逐步把重心转移到国内来，为每位科研人员献上高品质的产品及服务是我们的使命。
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