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犬I型胶原蛋白（ColⅠ）elisa试剂盒
厦门仑昌硕生物科技有限公司 本公司集研发、生产、销售、服务及品牌代理为体的生物技术公司，目可提供各种抗体、免疫学相关的检测试剂盒、胎牛血清、动物抗血清等高品质产品及服务，涵盖分子生物学、细胞生物学、免疫学等生命科学域。价格实惠，质量有保证，请您放心选购，欢迎来电咨询！
犬I型胶原蛋白（ColⅠ）elisa试剂盒
Mammary gland is an important organ for milk synthesis ａnd secretion, ａnd the regulation of mammary gland development ａnd lactation function is closely related to cell connection ａnd E-cadherin is an important factor in cell connection ａnd signal transduction. It plays a certain role in the process of breast development ａnd lactation. However, at present, the research on the formation of early breast development structure ａnd related signal transduction pathway has not been fully carried out. The purpose of this study is to reveal the molecular mechanism ａnd signal transduction mechanism of E-cadherin in the process of breast development, lactation ａnd degradation by molecular biological means on the basis of a correct understanding of breast structure ａnd function. To provide useful information for the construction of mammary gland development ａnd lactation regulation network, in this study, Holstein cows were used as experimental objects, ａnd immunofluorescence triple labeling staining, fluorescence quantitative RT-PCR ａnd Western blotting techniques were used to detect the localization ａnd quantitative expression of E-cadherin,Beta-catenin ａnd Cyclin D1 in breast tissues of dairy cows at different developmental stages. The effects of cowherb seed ａnd dandelion on the expression of adhesion molecules in mammary epithelial cells were detected by fluorescence quantitative RT-PCR ａnd Western blotting. the Beta-catenin gene was silenced by RNA interference technology, ａnd the expression changes of E-cadherin ａnd Cyclin D1 in Wnt signaling pathway ａnd their effects on cell proliferation ａnd growth were detected.
样品的处理及保存方法
犬I型胶原蛋白（ColⅠ）elisa试剂盒
1、 血清－－－－-操作过程中避免任何细胞刺激。使用不含热原和内毒素的试管。收集血液后，1000×g离心10分钟将血清和红细胞迅速小心地分离。
2、 血浆－－－－-EDTA、柠檬酸盐、肝素血浆可用于检测。1000×g离心30分钟去除颗粒。
3、 细胞上清液－－-1000×g离心10分钟除颗粒和聚合物。
4、 组织匀浆－－－－-将组织加入适量生理盐水捣碎。1000×g离心10分钟，取上清液
5、 保存－－－－－－如果样品不立即使用，应将其分成小部分-70 ℃保存，避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒，检测先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。
公司介绍
犬I型胶原蛋白（ColⅠ）elisa试剂盒
厦门仑昌硕生物科技有限公司 本公司集研发、生产、销售、服务及品牌代理为体的生物技术公司，目可提供各种抗体、免疫学相关的检测试剂盒、胎牛血清、动物抗血清等高品质产品及服务，涵盖分子生物学、细胞生物学、免疫学等生命科学域。公司非常注重企业信息化的建设及企业平台的建设，我们内部采用了的信息处理平台，保证客户的需求可以准确、高效处理。公司成立之初，立足外贸，和部分欧美及亚非国家的客户建立了良好的合作，随着国内生物域的蓬勃发展，逐步把重心转移到国内来，为每位科研人员献上高品质的产品及服务是我们的使命。
AMOY LUNCHANGSHUO BIOTECH CO.,LTD.
