文章关键词：113721-87-2，7-AMCA NHS ester，AMCA-OSu，7-AMCA NHS 活化酯，荧光染料

产品简介：7-AMCA NHS ester是一种广泛应用于蛋白质印迹、免疫测定和其他生物化学分析中的重要荧光染料。它的结构中包含氨基甲基香豆素和N-羟琥珀酰亚胺酯两个关键部分，这两个部分共同决定了其独特的性质和功能。

氨基甲基香豆素是一种具有良好灵敏度和低毒性的荧光染料，它能够与蛋白质的自由氨基基团特异性结合。这种结合是通过共价键的形成实现的，使得蛋白质被标记上荧光染料。这种标记方法具有很高的特异性和灵敏度，使得蛋白质的检测和定量变得非常准确和可靠。

N-羟琥珀酰亚胺酯是一种常用的活化剂，它能够将化学能转化为氨基基团的动能，从而增强其与目标物质的反应活性。在7-AMCA NHS ester中，N-羟琥珀酰亚胺酯的存在使得荧光染料能够更有效地与蛋白质结合，提高了标记效率。

除了在蛋白质印迹和免疫测定中的应用外，7-AMCA NHS ester还可以用于检测和定量生物样品中的其他生物分子，如核酸、糖等。此外，它还可以用于研究生物分子的结构和功能，以及用于制备荧光探针和荧光共振能量转移（FRET）等研究中。

总的来说，7-AMCA NHS ester是一种非常重要的荧光染料，在生物化学分析中发挥着重要的作用。其独特的特点和功能使得它在多个域中都有广泛的应用景。

7-AMCA NHS ester is an important fluorescent dye widely used in protein blotting, immunoassay, ａnd other biochemical analyses. Its structure contains two key components, amino methyl coumarin ａnd N-hydroxysuccinimide ester, which together determine its unique properties ａnd functions.

Aminomethylcoumarin is a fluorescent dye with good sensitivity ａnd low toxicity, which can specifically bind to the free amino groups of proteins. This binding is achieved through the formation of covalent bonds, allowing proteins to be labeled with fluorescent dyes. This labeling method has high specificity ａnd sensitivity, making protein detection ａnd quantification very accurate ａnd reliable.

N-hydroxysuccinimide ester is a commonly used activator that can convert chemical energy into the kinetic energy of amino groups, thereby enhancing its reaction activity with the target substance. In the 7-AMCA NHS ester, the presence of N-hydroxysuccinimide ester enables fluorescent dyes to bind more effectively to proteins, improving labeling efficiency.

In addition to its application in protein blotting ａnd immunoassay, 7-AMCA NHS ester can also be used to detect ａnd quantify other biomolecules in biological samples, such as nucleic acids, sugars, etc. In addition, it can also be used to study the structure ａnd function of biomolecules, as well as in the preparation of fluorescent probes ａnd fluorescence resonance energy transfer (FRET) research.

Overall, 7-AMCA NHS ester is a very important fluorescent dye that plays an important role in biochemical analysis. Its unique characteristics ａnd functions make it widely applicable in multiple fields.

中文名：7-AMCA NHS 活化酯

英文名：7-AMCA NHS ester，AMCA-OSu

CAS号：113721-87-2

化学式：C16H14N2O6

分子量：330.3

结构式：

外观：固体/粉末

规格：10mg、25mg、50mg等（接受各种规格的定制服务，具体可以线上咨询）

供应商：陕西新研博美生物科技有限公司

纯度：95%+

储存条件：本品应密封避光，储存于阴凉、干燥、通风处，避免反复冻融，储液应该立即使用

注意事项：试剂仅用于科学研究或者工业应用等非医疗目的，非药用，非食用！

试剂相关系列产品：

Acid-PEG9-NHS ester

Acid-PEG25-NHS ester

Acid-PEG13-NHS ester

endo-BCN-PEG12-NHS ester

Methyltetrazine-NHS ester

Bis-sulfone NHS Ester

DBCO-NHS ester 3

endo-BCN-PEG8-NHS ester

Bis-sulfone-PEG8-NHS Ester

Cyanine5.5 NHS ester

Cyanine7 NHS ester

Cy3.5 NHS ester

Cyanine7.5 NHS ester

Propargyl-NHS Ester

Bis-sulfone-PEG4-NHS Ester

endo-BCN-PEG4-NHS ester

endo-BCN-PEG3-NHS ester

t-Butoxycarbonyl-PEG2-NHS ester

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