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CBP73175 EGFR D770_N771insSVD/BaF3激酶细胞
点击次数：15发布时间：2023/4/30 17:07:46
更新日期：2023/4/30 17:07:46
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产品型号：CBP73175
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详细内容
| CBP73175 | |
| I. Introduction | |
Cell Line Name： | EGFR D770_N771insSVD/BaF3 |
Host Cell: | Ba/F3 |
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Application: | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
Freeze Medium: | 90% FBS+10% DMSO |
Complete Culture Medium: | RPMI-1640+10%FBS |
Mycoplasma Status: | Negative |
| II.Background | |
EGFR is the receptor for members of the EGF family and is a transmembrane glycoprotein that has tyrosine kinase activity. Binding of epidermal growth factor to EGFR induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation, differentiation, motility, and cell survival (1). Activation of EGFR triggers mitogenic signaling in gastrointestinal mucosa, and its expression is upregulated in colon cancers and most neoplasms (2). Activation of EGFR triggers activation of the ERK-signaling pathway in normal gastric epithelial and colon cancer cell lines. Inactivation of EGFR with selective inhibitors significantly reduces ERK2 activation, c-fos mRNA expression and cell proliferation. | |
| III. Representative Data | |
1.WB of EGFR D770_N771insSVD/BaF3
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2.Sanger of EGFR D770_N771insSVD/BaF3
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3. Anti-proliferation assay | |
Figure 3. Anti-proliferation assay of three reference compounds on the Ba/F3_EGFR D770_N771insSVD Stable Cell Line | |
