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| CBP60271 | |
| I. General information | |
| Synonyms: | KARPAS 299 |
| Background: | A human T cell lymphoma cell line established from the peripheral blood of a 25-year-old man with T cell non-Hodgkin's lymphoma in 1986; now classed as CD30+ anaplastic large cell lymphoma ALCL; cells carry the NPM-ALK fusion gene. |
| Species: | human Homo sapiens |
| Tissue: | Lymphoma |
| Disease: | Human Non-Hodgkin's Ki-positive Large Cell Lymphoma |
| Gender: | 25-year-old, male |
| Morphology: | Lymphoblastoid |
| Growth Mode: | Suspension |
| Doubling Time: | N/A |
| DNA Profile: | Amelogenin: X,Y CSF1PO: 10,11 D13S317: 8,12 D16S539: 12 D5S818: 11,12 D7S820: 10,11 THO1: 7,8.3 TPOX: 8 vWA: 17,19 Cobioer’s Cell Line Authentication Service |
| Culture Medium: | RPMI-1640+10%FBS We strongly suggest to purchase the complete medium from Cobioer. |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Karyotype: | Hypodiploid |
| Subculture Routine: | Maintain cultures between 0.5 - 2 x1000,000 cells/ml; 5% CO2; 37°C. Doubling time approximately 30 hours. Split saturated culture 1:3 every 2-3 days. Round to polygonal cells growing singly or in small clusters in suspension. |
| Comments: | For more information, please contact Cobioer 4008-750-250. |
