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CBP60721 HEL人红白血病细胞
点击次数：8发布时间：2023/7/6 15:00:32
更新日期：2023/7/6 15:00:32
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产品型号：CBP60721
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详细内容
HEL人红白血病细胞
| CBP60721 | |
| I. General information | |
| Synonyms: | HEL |
| Background: | established from the peripheral blood of a 30-year-old man with erythroleukemia AML M6 in relapse after treatment for Hodgkin lymphoma in 1980; cells were described in the literature to be capable of spontaneous and induced globin synthesis and to carry the JAK2 V617F mutation |
| Species: | human Homo sapiens |
| Tissue: | N/A |
| Disease: | erythroleukemia |
| Gender: | N/A |
| Morphology: | round, large to occasionally giant polynucleated, single cells in suspension; few cells adherent; |
| Growth Mode: | N/A |
| Doubling Time: | ca. 36 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: | RPMI-1640+10%FBS We strongly suggest to purchase the complete medium from Cobioer. |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | contamination was eliminated with Ciprobay ciprofloxacin, then negative in DAPI, microbiological culture, PCR assays |
| Immunology: | CD3 -, CD13 +, CD14 -, CD19 -, CD33 +, CD41a +, CD71 +, CD235a +; |
| Fingerprint: | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Species: | confirmed as human with IEF of AST, MDH, NP |
| Cytogenetics: | human hypotriploid karyotype with 2.3% polyploidy - 6360-64<3n>XYY, -2, -9, -10, -10, -11, -14, -16, -16, -17, -19, +20, +21, +2mar, del2q32, t3;6p13;q16, der5t5;17q10;q10, der6t1;6p13;p21, der7add7p14;q32, add8p21, der9t9;??;11p24;??;q13, del11q13, add15p11, del20q13, r20p11q11, dup21q11q22.3-qter, psu dic22;9t9;??;22p24;??;p11-13 - resembles published karyotype - carries also masked 5q- and 20q- consistent with AML M6/MDS |
| Molec. Genetics: | expression of mutated JAK2 confirmed by RT-PCR and sequencing |
| Virus | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer 4008-750-250. |
