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CBP60995 32D小鼠IL-3依赖性细胞
点击次数：5发布时间：2023/7/10 10:15:27
更新日期：2023/7/10 10:15:27
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产品型号：CBP60995
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详细内容
32D小鼠IL-3依赖性细胞
| CBP60995 | |
| I. General information | |
| Synonyms: | 32D |
| Background: | established from long-term bone marrow cultures of C3H/HeJ mice infected with the Friend murine leukemia virus; cells are constitutively growth factor-dependent |
| Species: | murine mouse Mus musculus |
| Tissue: | bone |
| Disease: | bone marrow |
| Gender: | N/A |
| Morphology: | mostly round cells growing singly or in small clusters in suspension, some cells are loosely adherent; |
| Growth Mode: | N/A |
| Doubling Time: | ca. 15 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: | IMDM + 10% FBS + 10% vol conditioned medium of cell line WEHI-3 OR +100ng/ml IL-3(R&D) We strongly suggest to purchase the complete medium from Cobioer. |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD11b +, F4/80 +; |
| Fingerprint: | N/A |
| Species: | originally confirmed as mouse with IEF of LDH, NP and with species PCR and recently as mus musculus by COI DNA Barcoding |
| Cytogenetics: | murine hypodiploid karyotype - 3534-35<2n>- carries 5 centric fusion markers |
| Virus | ELISA: reverse transcriptase negative; PCR: SMRV - |
| Comments: | For more information, please contact Cobioer 4008-750-250. |
