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CBP74061 OX40/NFkB-Luc/Jurkat细胞
点击次数：8发布时间：2023/8/16 10:23:33
更新日期：2023/8/16 10:23:33
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产品型号：CBP74061
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详细内容
OX40/NFkB-Luc/Jurkat
| CBP74061 | |
| I. Background | |
Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40, is a member of the TNFR-superfamily of receptors. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation. | |
| II. Description | |
| Recombinant Jurkat cell expressing firefly luciferase gene under the control of NF-κB response elements with constitutive expression of human OX40 (Tumor Necrosis Factor Receptor Superfamily Member 4, TNFRSF4, and CD134, GenBank Accession No. NM_003327) | |
| III. Introduction | |
| Host Cell: | Jurkat |
| Expressed gene: | OX40-NFkB-Luciferase |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | OX40, OX40 cell Line, CD134 Cell Line, ox40 nfkb |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+400ug/ml hygromycin+1ug/ml puromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Description of Host Cell Line | |
| Organism: | Human |
| Tissue: | Peripheral blood |
| Disease: | Childhood T acute lymphoblastic leukemia |
| Morphology: | Lymphoblast |
| Growth Properties: | Suspension |
| Ⅴ. Representative Data | |
| |
Figure 1. Recombinant Jurkat stably expressing human OX40. | |
Figure 2. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. Jurkat-OX40-NFkB-Luciferase Reporter cells were stimulated by OX40L,the S/B was 5.8-fold. |
