Mousealpha-enolase(ENO1)antibody(IgG)ELISAkit@2023资料已更新

ELISA（酶联免疫吸附测定）是一种基于多孔板的免疫测定法，将通常作为检测成分的抗体或样品吸附在固体表面（此方法采多孔板）。ELISA以相对低廉的成本，快速、定量且灵敏地检测分析物，是简单的分析方法。此外，ELISA还可轻松改造为更高通量筛选方法，帮助研究人员通过单次运行检测大量样品。夹心法、直接法和竞争法ELISAs

我们的ELISA采用简单易用的方法，可用于研究血清、血浆、细胞培养上清液或裂解液等各种基质中的可溶性蛋白标志物。包括1000多种“仅供研究使用”的检测试剂盒，适用的样本类型包括人、小鼠、大鼠等各种动物模型（如农业和伴生动物）。每份试剂盒都提供特定实验方案及96孔板所需的试剂。ELISA试剂盒主要为96孔规格，目标物检测和定量所需试剂都由厂商提供。采用夹心法、直接法和竞争法等各种ELISA方法。

夹心法使用针对同一目标抗原不同表位的一对单克隆抗体(mAb)。固定在微孔板中的一抗，用于将蛋白从溶液中提取出来。二抗则用于完成“夹心”，提供表示目标抗原存在的信号。试剂盒提供重组形式的已知含量的抗原蛋白，方便用户建立标准曲线解析样品信号。我们大部分产品采用的都是这种夹心法ELISA。

直接法和竞争法比较少见。直接法先用一种单抗检测固定在微孔板上的样品。然后一抗会与的酶标二抗结合提供信号。

竞争法在微孔板上预包被已知含量的生物标志物。酶标抗体与样品共同孵育，并与预包被抗体进行“竞争性”结合反应，具体结果取决于样品中目标物的浓度。于是游离抗体能与微孔板上的抗原结合，并在样品洗去后呈现信号。该信号与目标标志物的浓度成反比。

ELISA (enzyme-linked immunosorbent assay) is an immunoassay method based on porous plate, which adsorbs the antibody or sample that is usually one of the detection components on the solid surface (this method adopts porous plate). ELISA is one of the simplest analytical methods to detect analytes quickly, quantitatively ａnd sensitively at relatively low cost. In addition, ELISA can be easily transformed into a higher throughput screening method to help researchers detect a large number of samples through a single run. Sandwich method, direct method ａnd competition law ELISAs

Our ELISA adopts a simple ａnd easy method, which can be used to study soluble protein markers in various matrices such as serum, plasma, cell culture supernatant or lysate. It includes more than 1000 "research only" test kits, ａnd the applicable sample types include human, mouse, rat ａnd other animal models (such as agriculture ａnd associated animals). Each kit provides the reagents required for the specific experimental protocol ａnd 96-well plate. The ELISA kit is mainly of 96-well specification, ａnd the reagents required for target detection ａnd quantification are provided by the manufacturer. Various ELISA methods such as sandwich method, direct method ａnd competitive method were used.

Sandwich method uses a pair of monoclonal antibodies (mAb) targeting different epies of the same target antigen. The first antibody fixed in the microplate is used to extract the protein from the solution. The second antibody is used to complete the "sandwich" ａnd provide signals indicating the presence of the target antigen. The kit provides recombinant antigen protein with known content, which is convenient for users to establish standard curve to analyze sample signal. Most of our products use this sandwich ELISA.

Direct law ａnd competition law are relatively rare. The direct method first uses a monoclonal antibody to detect the sample fixed on the microplate. Then the first antibody will combine with the enzyme-labelled second antibody of to provide signals.

The competitive method pre-coated the known amount of biomarkers on the microplate. The enzyme-labeled antibody is incubated with the sample, ａnd carries out a "competitive" binding reaction with the pre-coated antibody. The specific result depends on the concentration of the target in the sample. The free antibody can then bind to the antigen on the microplate ａnd present a signal after the sample is washed. The signal is inversely proportional to the concentration of the target marker.

Mousealpha-enolase(ENO1)antibody(IgG)ELISAkit@2023资料已更新样本处理及要求：

1. 血清：将收集于血清分离管的全血标本在室温放置2小时或4℃过夜，然后1000×g离心20分钟，取上清即可，或将上清置于-20℃或-80℃保存，但应避免反复冻融。

2. 血浆：用EDTA或肝素作为抗凝剂采集标本，并将标本在采集后的30分钟内于2-8℃1000×g离心15分钟，取上清即可检测，或将上清置于-20℃或-80℃保存，但应避免反复冻融。

3. 组织匀浆：用预冷的PBS (0.01M, pH=7.4)冲洗组织，去除残留血液（匀浆中裂解的红细胞会影响测量结果），称重后将组织剪碎。将剪碎的组织与对应体积的PBS（一般按1:9的重量体积比，比如1g的组织样品对应9mL的PBS，具体体积可根据实验需要适当调整，并做好记录。推荐在PBS中加入蛋白酶抑制剂）加入玻璃匀浆器中，于冰上充分研磨。为了进一步裂解组织细胞，可以对匀浆液进行超声破碎，或反复冻融。将匀浆液于5000×g离心5~10分钟，取上清检测。

4. 细胞培养物上清：请1000×g离心20分钟，取上清即可检测，或将上清置于-20℃或-80℃保存，但应避免反复冻融。

5. 其它生物标本：1000×g离心20分钟，取上清即可检测。

6. 样品外观：样品应清澈透明，悬浮物应离心去除。

7. 样品保存：样品收集后若在1周内进行检测的可保存于4℃，若不能及时检测，请按一次使用量分装，冻存于-20℃（1个月内检测），或-80℃（6个月内检测），避免反复冻融，标本溶血会影响检测结果，因此溶血标本不宜进行此项检测。

Sensitivity: see the instructions

Test scope: see the instructions for details

The coefficient of variation within the board is less than 10%, ａnd the coefficient of variation between boards is less than 10%.

Equipment ａnd reagents to be prepared by oneself:

1. 450 ± 10nm filter enzyme marker

2. High-precision sampler ａnd gun head: 0.5-10uL, 2-20uL, 20-200uL, 200-1000uL

3. Eppendorf pipette

4. Distilled water or deionized water

5. absorbent cotton paper

6. 37 ℃ thermostat

8. Prepare several standard dilution tubes

ELISA (enzyme-linked immunosorbent assay) is an immunoassay method based on porous plate, which adsorbs the antibody or sample that is usually one of the detection components on the solid surface (this method adopts porous plate). ELISA is one of the simplest analytical methods to detect analytes quickly, quantitatively ａnd sensitively at relatively low cost. In addition, ELISA can be easily transformed into a higher throughput screening method to help researchers detect a large number of samples through a single run. Sandwich method, direct method ａnd competition law ELISAs

Our ELISA adopts a simple ａnd easy method, which can be used to study soluble protein markers in various matrices such as serum, plasma, cell culture supernatant or lysate. It includes more than 1000 "research only" test kits, ａnd the applicable sample types include human, mouse, rat ａnd other animal models (such as agriculture ａnd associated animals). Each kit provides the reagents required for the specific experimental protocol ａnd 96-well plate. The ELISA kit is mainly of 96-well specification, ａnd the reagents required for target detection ａnd quantification are provided by the manufacturer. Various ELISA methods such as sandwich method, direct method ａnd competitive method were used.

Sandwich method uses a pair of monoclonal antibodies (mAb) targeting different epies of the same target antigen. The first antibody fixed in the microplate is used to extract the protein from the solution. The second antibody is used to complete the "sandwich" ａnd provide signals indicating the presence of the target antigen. The kit provides recombinant antigen protein with known content, which is convenient for users to establish standard curve to analyze sample signal. Most of our products use this sandwich ELISA.

Direct law ａnd competition law are relatively rare. The direct method first uses a monoclonal antibody to detect the sample fixed on the microplate. Then the first antibody will combine with the enzyme-labelled second antibody of to provide signals.

The competitive method pre-coated the known amount of biomarkers on the microplate. The enzyme-labeled antibody is incubated with the sample, ａnd carries out a "competitive" binding reaction with the pre-coated antibody. The specific result depends on the concentration of the target in the sample. The free antibody can then bind to the antigen on the microplate ａnd present a signal after the sample is washed. The signal is inversely proportional to the concentration of the target marker.

Mousealpha-enolase(ENO1)antibody(IgG)ELISAkit@2023资料已更新需自备的设备及试剂：

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（本公司产品仅供体外研究使用，不用于临床诊断）

Mousealpha-enolase(ENO1)antibody(IgG)ELISAkit@2023资料已更新