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兔纤连蛋白(FN1)ELISA试剂盒@2023更新
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详细内容
产品名称:兔纤连蛋白(FN1)ELISA试剂盒
特色服务:提供免费代测,技术一对一指导。
规格:48T/96T
价格:请客服
样本:血清、血浆、细胞上清液、尿液、体液、灌洗液、脑脊髓、心房水、胸房水、组织等。
保存条件:2~8℃,zui佳温度6摄氏度,有效期6个月。
检测波长:450nm
研究域:炎症研究、神经生物学、新陈代谢、信号通路、免疫学、传染病、其他研究。
货期:ELISA试剂盒现货供应
提示:我司ELISA检测试剂盒仅供科研实验,不得用于其他用途;使用仔细阅读ELISA试剂盒说明书。
兔纤连蛋白(FN1)ELISA试剂盒@2023更新实验原理:
将目标抗体包被于微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的目标连接于固相载体上的抗体结合,然后加入微生物化的目标抗体,将未结合的生物素抗体洗净后,加入HRP标记和亲和素,再次洗涤后加入TMB底物显色。TMB在化物酶的催化下转化成蓝色,并在酸的作用下转化成zui终的黄色。颜色的深浅和样品中的目标呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。
兔纤连蛋白(FN1)ELISA试剂盒@2023更新组成及试剂配制
1、微孔酶标板(Micropore plate):一块(96孔/48孔)
2、标准品(Standard):六瓶
3、样本稀释液(Sample dilution):一瓶
4、检测抗体-HRP(Detection of antibodies-HRP):一瓶
5、20×洗涤缓冲液(20×Washing buffer):一瓶
6、底物A(Substrate A):一瓶
7、底物B(Substrate B):一瓶
8、终止液(Termination liquid):一瓶
9、封板膜(Sealing film):二张
服务:
一、有问题免费包换;
二、提供免费代测服务(为客户提供来样检测服务,zui大限度实验结果的有效性);
三、提供全程技术指导。
Product name: Rat neural cell adhesion molecule-1 (NCAM-1; CD56) ELISA test kit
Featured services: free agent testing and one-to-one technical guidance.
Specification: 48T/96T
Price: please customer service
Samples: serum, plasma, cell supernatant, urine, body fluid, lage fluid, cerebrospinal cord, atrial fluid, pleural fluid, tissue, etc.
Storage conditions: 2~8 ℃, zui good temperature 6 ℃, valid for 6 months.
Detection welength: 450nm
Research fields: inflammation research, neurobiology, metabolism, signal pathway, immunology, infectious diseases, and other research.
Delivery date: ELISA kit is ailable from stock
Prompt: Our ELISA test kit is only for scientific research and experiment, and cannot be used for other purposes; Read the instructions of ELISA kit carefully before use.
[Title] Experimental principle:
The target antibody is coated in the micro-well plate to make a solid carrier, and the standard or sample is added to the micro-well respectively. The target is connected to the antibody on the solid carrier, and then the target antibody is added to the microbiotic. After the unbound biotin antibody is washed, the HRP label and idin are added, and the TMB substrate is added to develop color after thorough washing again. TMB is converted into blue under the catalysis of peroxidase, and into yellow at zui end under the action of acid. The depth of color is positively correlated with the target in the sample. Measure the absorbance (O.D. value) with an enzyme marker at the welength of 450 nm and calculate the sample concentration.
[Title] Composition and reagent preparation
1. Micropore plate: one (96 holes/48 holes)
2. Standard: six bottles
3. Sample dilution: one bottle
4. Detection of antibodys-HRP: one bottle
5、20 × Washer buffer (20 × Washing buffer): one bottle
6. Substrate A: one bottle
7. Substrate B: one bottle
8. Termination liquid: one bottle
9. Sealing film: two
Service commitment:
1、 Product quality assurance, free replacement in case of problems;
2、 Provide free agent testing service (provide customers with sample testing service, and the validity of Zui large-limit test results);
3、 Provide technical guidance throughout the process.
兔纤连蛋白(FN1)ELISA试剂盒@2023更新