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教学型叶绿素荧光仪PAM-210
点击次数:405发布时间:2009/11/2 0:00:00
更新日期:1900/1/1 0:00:00
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详细内容
pam-210 —— 一台不仅仅是用于教学的荧光仪
schreiber教授因发明pam系列调制叶绿素荧光仪而获得首届光合作用协会(ispr)创新奖
调制叶绿素荧光技术尽管在科研上得到了广泛且成功的应用,但由于仪器昂贵,因此在很长一段时间里并没能用于教学实验。为此,schreiber教授专门设计了一台为本科生教学用的调制荧光仪——pam-210(也叫teaching-pam)。这台仪器的基本功能与pam-2100相同,其电脑操作软件也采用pamwin,同时还具有强大的单机操作功能。因此,pam-210不仅在教学应用上取得了成功,在科研领域也得到了成功应用。 schreiber教授专为利用pam-210进行教学实验写了一本《叶绿素荧光基础实验指南》,我司已将该书翻译成中文,供国内学者参考。 |
1)pam-2100的超浓缩版
2)可单机操作,可连电脑操作(操作软件与pam-2100相同)
3)可测荧光诱导曲线的快速上升动力学o-i-d-p相
4)可测荧光诱导曲线的慢速下降动力学并进行淬灭分析
5)可测光响应曲线和快速光曲线(rlc)
6)研究对象包括高等植物和藻类
7)专为针叶植物配了叶室
8)利用微光纤可检测超小样品的光合作用
测量参数
fo、fm、fv/fm、ft、fm’、fo’、δf/fm’、qp、qn、npq和retr等。
应用领域
为本科教学实验设计,但具备了pam的所有基本功能,可用于植物生理学、植物生态学、农学、园艺学、水生生物学等领域。
带磁性暗适应叶室 | 连接微光纤 | 带特制针叶叶室 | 带液体样品室 |
技术参数
测量光:红色发光二极管(led),650 nm,标准强度0.04 μmol m-2 s-1par;调制频率32hz或8 khz,自动转换。
光化光:红色led,665 nm,连续光强1800 μmol m-2 s-1par
饱和脉冲:红色led,665 nm,连续光强3500 μmol m-2 s-1par
远红光:led,730 nm,强度10 w m-2。
信号检测:pin-光电二极管,带短波截止滤光片(λ>710 nm);选择性锁相放大器(设计)。
环境温度:-5~+45℃,在极地研究中成功应用
部分文献
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2. pieruschka r, schurr u, jensen m, wolff wf, jahnke s, lateral diffusion of co2 from shaded to illuminated leaf parts affects photosynthesis inside homobaric leaves. new phytologist, 2006. 169: 779-788.
3. shalygo nv, kozel nv, effect of bengal rose sensitizer on activity of the photosynthetic apparatus of green barley leaves journal of applied spectroscopy, 2006. 73(2): 301-304.
4. 肖月娥, 陈开宁, 戴新宾, 陈小峰, 许晓明, 太湖中2种大型沉水被子植物适应低光能力的比较. 植物生理学通讯, 2006. 42(3): 421-425.
5. bannister p, maegli t, dickinson kjm, halloy srp, knight a, lord jm, mark af, spencer kl, will loss of snow cover during climatic warming expose new zealand alpine plants to increased frost damage? . oecologia, 2005. 144(2): 245-256.
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12. kudoh s, kashino y, imura s, ecological studies of aquatic moss pillars in antarctic lakes 3. light response and chilling and heat sensitivity of photosynthesis. polar bioscience, 2003. 16: 33-42.
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14. pshibytko nl, kalitukho ln, kabashnikova lf, effects of high temperature and water deficit on photosystem ii in hordeum vulgare leaves of various ages russian journal of plant physiology, 2003. 50(1): 44-51.
15. beer dd, larkum awd, photosynthesis and calcification in the calcifying algae halimeda discoidea studied with microsensors. plant cell and environment, 2001. 24(11): 1209-1217.
16. eckert m, kaldenhoff r, light-induced stomatal movement of se-lected arabidopsis thaliana mutants. journal of experimental botany, 2000. 51(349): 1435-1442.
17. shi l-x, lorkovi zj, oelmüller r, schröder wp, the low molecular mass psbw protein is involved in the stabilization of the dimeric photosystem ii complex in arabidopsis thaliana. the journal of biological chemistry, 2000. 275(48): 37945-37950.
18. christen g, stevens g, lukins pb, renger g, larkum awd, isolation and characterisation of oxygen evolving thylakoids from the marine prokaryote prochloron didemni febs letters, 1999. 449(2-3): 264-268.
19. jones rj, hoegh-guldberg o, larkum awd, schreiber u, temperature-induced bleaching of corals begins with impairment of the co2 fixation mechanism in zooxanthellae. plant cell and environment, 1998. 21: 1219-1230.
20. salih a, hoegh-guldberg o, cox g. bleaching responses of symbiotic dinoflagellates in corals: the effects of light and elevated temperature on their morphology and physiology. in proceedings of the australian coral reef society 75th anniversary conference. 1997. heron island: the university of queensland, brisbane.
21. schreiber u, chlorophyll fluorescence and photosynthetic energy conversion: simple introductory experiments with the teaching-pam chlorophyll fluorometer. 1997, effeltrich, germany: heinz walz gmbh.