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菌种库: 微生物菌种; CICC菌种; CMCC菌种; ATCC菌种

细胞库: 自然杀伤细胞; 其它动物细胞; 大/小鼠肿瘤、癌细胞; 大/小鼠正常细胞; 人肿瘤、癌细胞; 人正常细胞

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C2C12小鼠成肌细胞

小鼠成肌细胞

点击次数：502 发布时间：2011/11/9[更新时间:2024/11/14 14:28:39]

型号： C2C12
价格：面议
所在地点：美洲
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简单介绍： CRL-1772 C2C12 小鼠成肌细胞，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和培养条件！

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CRL-1772 C2C12 小鼠成肌细胞

ATCC^® Number:

CRL-1772™

Price:

$256.00

Designations:	C2C12
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	adherent
Organism:	Mus musculus (mouse)
Morphology:	myoblast
Source:	Strain: C3H Tissue: muscle Cell Type: myoblast;
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications:	transfection host (Nucleofection technology from Lonza Roche FuGENE® Transfection Reagents)
Comments:	This is a subclone (produced by H. Blau, et al) of the mouse myoblast cell line established by D. Yaffe ａnd O. Saxel. [22903] The C2C12 cell line differentiates rapidly, forming contractile myotubes ａnd producing characteristic muscle proteins. [22953] Treatment with bone morphogenic protein 2 (BMP-2) cause a shift in the differentiation pathway from myoblastic to osteoblastic. [23427] Tested ａnd found negative for ectromelia virus (mousepox).
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco＇s Modified Eagle＇s Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C
Subculturing:	Protocol: IMPORTANT - DO NOT ALLOW CULTURES TO BECOME CONFLUENT. Cultures must not be allowed to become confluent as this will deplete the myoblastic population in the culture. Myotube formation is enhanced when the medium is supplemented with 10% horse serum instead of fetal bovine serum. Remove ａnd discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask ａnd observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium ａnd aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Inoculate at a cell concentration between 1.5 X 10 exp5 ａnd 1.0 X 10 exp6 viable cells/75 cm2. Incubate cultures at 37°C.

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