ATCC® Number:  HTB-113™      
Designations:  HEC-1-B 
Depositors:   H Kuramoto 
Biosafety Level: 1 
Shipped:  frozen 
Medium & Serum:  See Propagation 
Growth Properties: adherent
Organism: Homo sapiens （human） 
Morphology: epithelial

 
Source: Organ: uterus
Tissue: endometrium
Disease: adenocarcinoma
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 HTB-113 HEC-1-B 人子宫内膜腺癌细胞
Applications: transfection host （Roche FuGENE® Transfection Reagents）
Tumorigenic: Yes 
Antigen Expression: Blood Type B; Rh+
DNA Profile （STR）: Amelogenin: X
CSF1PO: 10,12
D13S317: 11,16
D16S539: 11,12
D5S818: 11,13
D7S820: 9,11
THO1: 6,7
TPOX: 8,11
vWA: 18
Cytogenetic Analysis: diploid to tetraploid with large submetacentric marker
Isoenzymes:  AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 2
PGM1, 1
PGM3, 1-2
Age:  71 years 
Gender:  female 
Comments: This is a substrain of HEC-1-A （see ATCC HTB-112） isolated in 1968 by H. Kuramoto.
Unlike HEC-1-A, this substrain exhibited a stationary growth period between the 135th ａnd 190th days in culture ａnd appeared on recovery to be flattened ａnd more pavement patterned than the parent line.
Futhermore, the predominant complement of chromosomes was double that observed for the parent line.
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle＇s Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing:  Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, ａnd rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution ａnd add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature （or at 37C） until the cells detach.
Add fresh culture medium, aspirate ａnd dispense into new culture flasks.
Preservation:  Culture medium, 95%; DMSO, 5%
Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2003
recommended serum:ATCC 30-2020
References: 22449: Kuramoto H. Studies of the growth ａnd cytogenetic properties of human endometrial adenocarcinoma in culture ａnd its development into an established line. Acta Obstet. Gynaecol. Jpn. 19: 47-58, 1972. PubMed: 4678779
22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
22539: Fogh J, et al. One hundred ａnd twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
23069: Presta M, et al. Modulation of plasminogen activator activity in human endometrial adenocarcinoma cells by basic fibroblast growth factor ａnd transforming growth factor beta. Cancer Res. 48: 6384-6389, 1988. PubMed: 3263185
23541: Kuramoto H, et al. Establishment of a cell line of human endometrial adenocarcinoma in vitro. Am. J. Obstet. Gynecol. 114: 1012-1019, 1972. PubMed: 4673779
32299: St. Geme JW, et al. Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J. Bacteriol. 178: 6281-6287, 1996. PubMed: 8892830
33112: Schramm N, et al. Vesicles containing Chlamydia trachomatis serovar L2 remain above pH 6 within HEC-1B cells. Infect. Immun. 64: 1208-1214, 1996. PubMed: 8606080
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HTB-113 HEC-1-B 人子宫内膜腺癌细胞