| Designations: | MIA PaCa-2 |
| Depositors: | A Yunis |
| Biosafety Level: | 1 |
| Shipped: | frozen |
| Medium & Serum: | See Propagation |
| Growth Properties: | adherent, single cells ａnd loosely attached clusters |
| Organism: | Homo sapiens （human） |
| Morphology: | attached epithelial with floating rounded cells
|
| Source: | Organ: pancreas
Disease: carcinoma |
| Cellular Products: | human colony stimulating factor, subclass I （CSF-I）; plasminogen activator |
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
| CRL-1420 MIAPaCa-2 胰腺癌细胞系 |
| Isolation: | Isolation date: 1975 |
| DNA Profile （STR）: | Amelogenin: X
CSF1PO: 10
D13S317: 12,13
D16S539: 10,13
D5S818: 12,13
D7S820: 12,13
THO1: 9,10
TPOX: 9
vWA: 15 |
| Cytogenetic Analysis: | This is a hypotriploid human cell line. The modal chromosome number is 61. Sixteen to 20 marker chromosomes are commonly found in a cell. A few normal chromosomes are absent. |
| Isoenzymes: | G6PD, B |
| Age: | 65 years |
| Gender: | male |
| Ethnicity: | Caucasian |
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco＇s Modified Eagle＇s Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%; horse serum to a final concentration of 2.5%.
Temperature: 37.0°C |
| Subculturing: | Protocol: Remove medium, ａnd rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution ａnd add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature （or at 37C） until the cells detach. Add fresh culture medium, aspirate ａnd dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week |
| Preservation: | Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase |
| Related Products: | Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2002
recommended serum:ATCC 30-2020
recommended serum:ATCC 30-2040 |
| References: | 1057: Wu M, et al. Purification ａnd characterization of a plasminogen activator secreted by cultured human pancreatic carcinoma cells. Biochemistry 16: 1908-1913, 1977. PubMed: 15590
22291: Fountzilas G, et al. Comparative effects of selected drug combinations on the growth of a human pancreatic carcinoma cell line （MIA PaCa-2）. J. Natl. Cancer Inst. 76: 37-43, 1986. PubMed: 3455740
49623: Robertson JF, et al. Effect of gastrointestinal hormones ａnd synthetic analogues on the growth of pancreatic cancer. Int. J. Cancer 63: 69-75, 1995. PubMed: 7558455
58034: Yunis AA, et al. Human pancreatic carcinoma （MIA PaCa-2） in continuous culture: sensitivity to asparaginase. Int. J. Cancer 19: 128-135, 1977. PubMed: 832918 |
