| Designations: | HK-2 |
| Depositors: | RA Zager |
| Biosafety Level: | 2 [Cells Contain Papilloma viral DNA sequences ] |
| Shipped: | frozen |
| Medium & Serum: | See Propagation |
| Growth Properties: | adherent |
| Organism: | Homo sapiens （human） |
| Morphology: | epithelial
|
| Source: | Organ: kidney, cortex
Tissue: proximal tubule
Cell Type: human papillomavirus 16 （HPV-16） transformed |
| Cellular Products: | alkaline phosphatase; gamma glutamyltranspeptidase; leucine aminopeptidase; acid phosphatase; cytokeratin; alpha 3, beta 1 integrin; fibronectin |
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
| CRL-2190 HK-2 人肾近曲小管上皮细胞 |
| Receptors: | epidermal growth factor （EGF）, expressed |
| DNA Profile （STR）: | Amelogenin: X,Y
CSF1PO: 13
D13S317: 9
D16S539: 11,12
D5S818: 12
D7S820: 10,11
THO1: 9
TPOX: 8,9
vWA: 17,18 |
| Age: | adult |
| Gender: | male |
| Propagation: | ATCC complete growth medium: The base medium for this cell line is provided by Invitrogen （GIBCO） as part of a kit: Keratinocyte Serum Free Medium （K-SFM）, Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line （bovine pituitary extract （BPE） ａnd human recombinant epidermal growth factor （EGF）. To make the complete growth medium, you will need to add the following components to the base medium: - 0.05 mg/ml BPE - provided with the K-SFM kit
- 5 ng/ml EGF - provided with the K-SFM kit. NOTE: Do not filter complete medium.
Atmosphere: air, 95%; carbon dioxide （CO2）, 5%
Temperature: 37.0°C
Growth Conditions: Cell growth is dependent on epidermal growth factor. The cells should not be allowed to become confluent. Subculture at 80% of confluence. |
| Subculturing: | Protocol: - Remove ａnd discard culture medium.
- Briefly rinse the cell layer with 0.05% （w/v） Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
- Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask ａnd observe cells under an inverted microscope until cell layer is dispersed （usually within 5 to 15 minutes）.
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. - Add 6.0 to 8.0 ml of complete growth medium ａnd aspirate cells by gently pipetting.
- To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube ａnd spin at approximately 125 xg for 5 to 10 minutes.Discard supernatant ａnd resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.
- Incubate cultures at 37°C.
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