RAW 264.7小鼠单核巨噬细胞白血病细胞
ATCC® Number:  TIB-71™       
Designations:  RAW 264.7  
Depositors:   WC Raschke  
Biosafety Level: 2  
Shipped:  frozen  
Medium & Serum:  See Propagation  
Growth Properties: adherent 
Organism: Mus musculus (mouse)  
Morphology: monocyte/macrophage

 
Source: Tissue: ascites 
Strain: BALB/c 
Disease: Abelson murine leukemia virus-induced tumor 
Cell Type: macrophage; Abelson murine leukemia virus transformed 
Cellular Products: lysozyme [1207]  
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Applications: Biological response [92560] 
transfection host (Roche FuGENE® Transfection Reagents) 
Receptors: complement (C3) [1207] 
Antigen Expression: H-2d 
Age:  *     RAW 264.7小鼠单核巨噬细胞白血病细胞 
Gender:  male  
Comments: This line was established from a tumor induced by Abelson murine leukemia virus. They are negative for surface immunoglobulin (sIg-), Ia (Ia-) ａnd Thy-1.2 (Thy-1.2)This line does not secrete detectable virus particles ａnd is negative in the XC plaque formation assay. The cells will pinocytose neutral red ａnd will phagocytose latex beads ａnd zymosan. They are capable of antibody dependent lysis of sheep erythrocytes ａnd tumor cell targets. LPS or PPD treatment for 2 days stimulates lysis of erythrocytes but not tumor cell targets.Data communicated in Feb. 2007 by Dr Janet W. Hartley, indicates the expression of infectious ecotropic MuLV closely related, if not identical, to the Moloney MuLV helper virus used in the original virus inoculum. The cells also express polytropic MuLV, unsurprisingly based on the mouse passage history of the virus stocks [ PubMed 18177500]. 
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco＇s Modified Eagle＇s Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
Temperature: 37.0°C 
Subculturing:  Protocol: Subcultures are prepared by scraping.
For a 75 cm2 flask, remove all but 10 ml culture medium (adjust amount accordingly for other culture vessels). Dislodge cells from the flask substrate with a cell scraper; aspirate ａnd add appropriate aliquots of the cell suspension into new culture vessels. 
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended 
Medium Renewal: Replace or add medium every 2 to 3 days. 
Preservation:  Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO 
Storage temperature: liquid nitrogen vapor phase 
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020 
References: 1135: Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte ａnd tumor targets by macrophage-related cell lines: enhancement by PPD ａnd LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031
1207: Raschke WC, et al. Functional macrophage cell lines transformed by Abelson leukemia virus. Cell 15: 261-267, 1978. PubMed: 212198
32443: Denlinger LC, et al. Regulation of inducible nitric oxide synthase expression by macrophage purinoreceptors ａnd calcium. J. Biol. Chem. 271: 337-342, 1996. PubMed: 8550583
32466: Hambleton J, et al. Activation of c-Jun N-terminal kinase in bacterial lipopolysaccharide-stimulated macrophages. Proc. Natl. Acad. Sci. USA 93: 2774-2778, 1996. PubMed: 8610116
32553: Taylor GA, et al. Identification of anovel GTPase, the inducibly expresed GTPase, that accumulates in response to interferon gamma. J. Biol. Chem. 271: 20399-20405, 1996. PubMed: 8702776
32901: Li YM, et al. Molecular identity ａnd cellular distribution of advanced glycation endproduct receptors: relationship of p60 to OST-48 ａnd p90 to 80K-H membrane proteins. Proc. Natl. Acad. Sci. USA 93: 11047-11052, 1996. PubMed: 8855306    RAW 264.7小鼠单核巨噬细胞白血病细胞 
33046: Panneerselvam K, Freeze HH. Mannose enters mammalian cells using a specific transporter that is insensitive to glucose. J. Biol. Chem. 271: 9417-9421, 1996. PubMed: 8621609
33076: Lokuta MA, et al. Mechanisms of murine RANTES chemokine gene induction by newcatle disease virus. J. Biol. Chem. 271: 13731-13738, 1996. PubMed: 8662857
33162: Taylor MF, et al. In vitro efficacy of morpholino-modified antisense oligomers directed against tumor necrosis factor-alpha mRNA. J. Biol. Chem. 271: 17445-17452, 1996. PubMed: 8663413
92560: Standard Practice for Testing for Biological Responses to Particles in Vitro. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 1903-98R03.
16173094: Hartley JW, et al. Expression of infectious murine leukemia viruses by RAW264.7 cells, a potential complication for studies with a widely used mouse macrophage cell line. Retrovirology. 4: 5:1, 2008. PubMed 18177500. 
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