| Designations: | SK-OV-3 [SKOV-3] | | Depositors: | G Trempe, LJ Old | | Biosafety Level: | 1 | | Shipped: | frozen | | Medium & Serum: | See Propagation | | Growth Properties: | adherent | | Organism: | Homo sapiens （human） | | Morphology: | epithelial
| | Source: | Organ: ovary
Disease: adenocarcinoma
Derived from metastatic site: ascites | | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | | HTB-77 SK-OV-3 人卵巢癌细胞 | | Restrictions: | The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.） The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.） Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone （212） 639-6181; FAX （212） 717-3439. | | Isolation: | Isolation date: 1973 | | Applications: | transfection host （Nucleofection technology from Lonza
Roche FuGENE® Transfection Reagents） | | Tumorigenic: | Yes | | Antigen Expression: | Blood Type B; Rh+ | | DNA Profile （STR）: | Amelogenin: X
CSF1PO: 11
D13S317: 8,11
D16S539: 12
D5S818: 11
D7S820: 13,14
THO1: 9,9.3
TPOX: 8,11
vWA: 17,18 | | Cytogenetic Analysis: | This is a hypodiploid human cell line. The modal chromosome number was 43, occurring in 63.3% of cells. The range was 42 to 45. The rate of higher ploidies was 32%. The del（1）（q21）, der（13）t（1;?;13） （q11;?;q34）, der（11）t（11;?） （q12）, del（10）（q22） ａnd 3 other marker chromosomes were common to most cells, ａnd 3 others were found only in some cells. One N11 had the HSR segment from p11 to the distal end. The normal N10, N12, N15, N17 ａnd N19 were absent. Others were either single or paired. There were from 1 to 6 rearranged ａnd unassignable chromosomes. The X chromosome was either single or paired. | | Isoenzymes: | AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1-2
Me-2, 1
PGM1, 1-2
PGM3, 1 | | Age: | 64 years | | Gender: | female | | Ethnicity: | Caucasian | | Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy＇s 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide （CO2）, 5%
Temperature: 37.0°C | | Subculturing: | Protocol: - Remove ａnd discard culture medium.
- Briefly rinse the cell layer with 0.25% （w/v） Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
- Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask ａnd observe cells under an inverted microscope until cell layer is dispersed （usually within 5 to 15 minutes）.
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. - Add 6.0 to 8.0 ml of complete growth medium ａnd aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week | | Preservation: | Freeze medium: Complete growth medium supplemented with 5% （v/v） DMSO
Storage temperature: liquid nitrogen vapor phase | | Related Products: | Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2007
recommended serum:ATCC 30-2020 | | References: | 21869: . Human tumor cells in vitro. New York: Plenum Press; 1975.
22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
22539: Fogh J, et al. One hundred ａnd twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
23103: Morimoto H, et al. Overcoming tumor necrosis factor ａnd drug resistance of human tumor cell lines by combination treatment with anti-Fas antibody ａnd drugs or toxins. Cancer Res. 53: 2591-2596, 1993. PubMed: 7684321
23478: Morimoto H, et al. Synergistic effect of tumor necrosis factor-alpha- ａnd diphtheria toxin- mediated cytotoxicity in sensitive ａnd resistant human ovarian tumor cell lines. J. Immunol. 147: 2609-2616, 1991. PubMed: 1918981
32281: Zhang X, et al. Microfilament depletion ａnd circumvention of multiple drug resistance by sphinxolides. Cancer Res. 57: 3751-3758, 1997. PubMed: 9288783
32456: Clinton GM, et al. Estrogens increase the expression of fibulin-1, an extracellular matrix protein secreted by human ovarian cancer cells. Proc. Natl. Acad. Sci. USA 93: 316-320, 1996. PubMed: 8552629
32530: Zhang X, Smith CD. Microtubule effects of welwistatin, a cyanobacterial indoli that circumvents multiple drug resistance. Mol. Pharmacol. 49: 288-294, 1996. PubMed: 8632761
90272: Wiechen K, et al. Suppression of the c-erbB-2 gene product decreases transformation abilities but not the proliferation ａnd secretion of proteases of SK-OV-3 ovarian cancer cells. Br. J. Cancer 81: 790-795, 1999. PubMed: 10555747
90273: Yu D, et al. Enhanced c-erbB-2/neu expression in human ovarian cancer cells correlates with more severe malignancy that can be suppressed by E1A. Cancer Res. 53: 891-898, 1993. PubMed: 8094034
90274: Karlan BY, et al. Glucocorticoids stabilize HER-2/neu messenger RNA in human epithelial ovarian carcinoma cells. Gyn. Onc. 53: 70-77, 1994. PubMed: 7909787 | HTB-77 SK-OV-3 人卵巢癌细胞 |
