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当前位置: 易推广 > 生物试剂/抗体/细胞 > 生物制剂 > 其他 > 上海复祥生物科技有限公司 > 产品展示 > 细胞库 > 人肿瘤、癌细胞 > 人神经母细胞瘤细胞

SK-N-BE(2)人神经母细胞瘤细胞

人神经母细胞瘤细胞

点击次数:1230  发布时间:2011/11/25[更新时间:2024/11/14 14:28:39]

  • 型 号: SK-N-BE(2)
  • 价 格:面议
  • 所在地点:美洲
  • 产品完善度:

简单介绍: CRL-2271 SK-N-BE(2) 人神经母细胞瘤细胞,ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和培养条件!
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CRL-2271 SK-N-BE(2) 人神经母细胞瘤细胞
ATCC® Number: CRL-2271™    Price:  
Designations: SK-N-BE(2)
Depositors:  JL Biedler
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: mixed, adherent and suspension
Organism: Homo sapiens (human)
Morphology: neuroblast

Source: Organ: brain
Disease: neuroblastoma
Derived from metastatic site: bone marrow
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 CRL-2271 SK-N-BE(2) 人神经母细胞瘤细胞
Restrictions: NOTE: SK-N-BE(2) was deposited at the ATCC by June L. Biedler, Memorial Sloan-Kettering Cancer Center. SK-N-BE(2) is distributed for academic research purposes only. Memorial Sloan-Kettering releases the line subject to the following: 1.)SK-N-BE(2) or its products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of SK-N-BE(2) including any use by a for-profit entity must first be negotiated with Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.
Isolation: Isolation date: November, 1972
Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10
D13S317: 11
D16S539: 9,11
D5S818: 12
D7S820: 9,10
THO1: 6,7
TPOX: 8,11
vWA: 18
Cytogenetic Analysis: modal number = 44; one or more HSR bearing chromosomes
Age: 2 years
Gender: male
Comments: The SK-N-BE(2) neuroblastoma cell line was established in November of 1972 from a bone marrow biopsy taken from child with disseminated neuroblastoma after repeated courses of chemotherapy and radiotherapy.
The cells exhibit moderate levels of dopamine beta hydroxylase activity.
SK-N-BE(2) cells have a reported saturation density greater than 1 X 10 exp6 cells/sq cm.
The morphology of the cells varies with some cells having long processes and others that are epithelioid like.
The cells will aggregate, form clumps and float.
Propagation: ATCC complete growth medium: The base medium for this cell line is a 1:1 mixture of ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003, and F12 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10.
Temperature: 37.0°C
Subculturing: Protocol: These cells grow as a mixture of floating and adherent cells. Remove the medium with the floating cells, and recover the cells by centrifugation. Rinse the adherent cells with fresh 0.25 trypsin, 0.53 mM EDTA solution, add an additional 1 to 2 ml of trypsin solution, and let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate, combine with the floating cells recovered above and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:12 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 95; DMSO, 5
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 30 hrs
Related Products: recommended serum:ATCC 30-2020
References:

 

 

CRL-2271 SK-N-BE(2) 人神经母细胞瘤细胞

上海复祥生物提供 ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|,细胞库管理规范,提供的细胞株背景清楚,提供参考文献和培养条件,网站上有细胞照片,欢迎各位老师来电咨询!
iec-6 大鼠小肠隐窝上皮细胞
马-达二氏犬肾细胞系 MDCK (NBL-2) CRL-34
鸭子胚胎成纤维细胞  Duck embryo  CCL-141
小鼠胚胎成纤维细胞 3T3-L1 CCL-173
人结肠直肠腺癌 COLO 320DM CCL-220
人结肠直肠腺癌 NCI-H508 CRL-253
人结肠直肠腺癌 COLO 320HSR CCL-220.1
人B淋巴细胞 Ramos(RA1) CRL-1596
人淋巴母细胞 T2(174×CEM.T2) CRL-1992
大鼠心肌细胞 H9C2(2-1) CRL-1446
LM-3 人肝癌细胞系
猪鼻甲黏膜成纤维细胞 PT-K75 CRL-2528
小鼠胰腺癌细胞 LTPA CRL-2389
大鼠胰腺癌细胞 DSL-6A/C1  CRL-2132
小鼠骨髓瘤B淋巴细胞 SP2/MIL-6 CRL-2016
大鼠脑间质细胞 DI TNC1 CRL-2005
小鼠肌原细胞 C2C12 CRL-1772
鹌鹑纤维肉瘤细胞 QT6 CRL-1708
大鼠回肠细胞 IEC-18 CRL-1589
非洲绿猴肾细胞 VERO C1008 [Vero 76, clone E6, Vero E6]  CRL-1586
人成纤维细胞 Hs 738.St/Int CRL-7869
人正常前列腺上皮细胞 WPE-int CRL-2888
人正常前列腺成肌纤维细胞 WPMY-1 CRL-2854
人胰腺导管腺癌细胞 PL45 CRL-2558
人胰腺癌细胞 Panc 05.04 CRL-2557
人胰腺癌细胞 Panc 04.03  CRL-2555
人胰腺癌细胞 Panc 02.03 CRL-2553
人胰腺癌细胞 Panc 08.13 CRL-2551
人胰腺癌细胞 Panc 03.27 CRL-2549
人胰腺癌细胞 Panc 10.05 CRL-2547
人肝癌细胞 SNU-398  CRL-2233
人成神经瘤细胞-骨髓 SK-N-DZ CRL-2149
人胰腺癌细胞 HPAC CRL-2119
人胰腺癌细胞 HPAF-II CRL-1997
人黑色素瘤细胞 A375.S2 CRL-1872
人脐静脉内皮细胞 HUV-EC-C CRL-1730
人黑素瘤细胞 WM-266-4 CRL-1676
人膀胱癌细胞 HT-1376  CRL-1472
人正常前列腺上皮细胞 RWPE-2 CRL-11610
人正常前列腺上皮细胞 RWPE-1 CRL-11609
鸡胚胎成纤维细胞 UMNSAH/DF-1 CRL-12203
人乳腺癌细胞 MDA-MB-231 HTB-26
小鼠巨噬细胞 RAW 264.7 TIB-71
人横纹肌肉瘤细胞 A-204 HTB-82
人胰腺癌细胞 Capan-2  HTB-80
人胰腺癌细胞 Capan-1 HTB-79
人乳腺导管癌细胞 MDA-MB-435S HTB-129
人胃癌细胞 Hs 746T HTB-135
人肺腺癌细胞 NCI-H441 [H441] HTB-174
人骨髓瘤细胞 U266  TIB-196
人结肠癌细胞 NCI-H716 [H716] CCL-251
人多形性恶性胶质瘤 T98G [T98-G] CRL-1690
人神经母细胞瘤细胞 BE(2)-C CRL-2268
人肾透明细胞癌细胞 Caki-2 HTB-47
人皮肤成纤维细胞 Hs 865.Sk CRL-7601
LM-3 人肝癌细胞系CA46 人淋巴瘤细胞系
CRL-5826 NCI-H226 人肺鳞癌细胞系
Tca-8113 人舌鳞癌细胞
MA-891 小鼠乳腺癌高转移细胞
U14 小鼠宫颈癌细胞
CRL-9518 L5178Y 小鼠淋巴瘤细胞
HTB-112 HEC-1-A 人子宫内膜腺癌细胞
HTB-113 HEC-1-B 人子宫内膜腺癌细胞
AN3 CA 人子宫内膜腺癌细胞
ROS1728 大鼠成骨细胞
CRL-6323 B16F1 小鼠黑色素瘤细胞
HTB-58 SK-MES-1 人肺鳞癌细胞
CCL-127 IMR-32 人神经母细胞瘤细胞
HTB-79 Capan-2 人胰腺癌细胞
BALL-1 人B淋巴细胞急性白血病细胞
CRL-2922 Eayh926 人脐静脉细胞融合细胞
WB-F344  大鼠肝干细胞
CRL-1715 TM4  正常小鼠睾丸细胞
CRL-2539 4T1 小鼠乳腺癌细胞
CRL-1442 BRL-3A 大鼠肝细胞 
BRL 大鼠肝细胞
RSC96大鼠雪旺细胞
Isk 人子宫内膜癌细胞
HTB-113 hec-1-b 人子宫内膜腺癌细胞
PLA-802?人恶性横纹肌肉瘤
Pt-K2 袋鼠肾细胞
BS-C-1 非洲绿猴肾细胞
A498人肾癌细胞
3AO人卵巢癌细胞
CRL-2271 SK-N-BE(2) 人神经母细胞瘤细胞

 

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