WEHI-231 (ATCC® CRL-1702™) 
Organism  Mus musculus, mouse  
Cell Type  B lymphocyte, immature  
Product Format  frozen  
Morphology  lymphoblast  
Culture Properties  suspension, multicell aggregates  
Biosafety Level  1  
Disease  B cell lymphoma  
Strain  (BALB/c x NZB)F1  
Applications  This cell line is a suitable transfection host.  
Storage Conditions  liquid nitrogen vapor phase  
Images    
Derivation  Mineral oil induced tumor in (BALB/c x NZB)F1 mice  
Genes Expressed  immunoglobulin 
Comments  WEHI-231 cells do not secrete IgM into medium unless stimulated with lipopolysaccharide (0.01 to 3 μg/mL). Tested ａnd found negative for ectromelia virus (mousepox).

Complete Growth Medium  The base medium for this cell line is ATCC-formulated Dulbecco＇s Modified Eagle＇s Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%. 
 
Subculturing  Optimum culture recovery from a frozen vial is in a T25 flask at a density between 2 to 5 X 105 viable cells/mL. If recovered at 5 X 105 viable cells/mL, media will need to be added to the culture within the first 3 days, before the density reaches 1 X 106 viable cells/mL, at which point the viability drops drastically. 
After cells recover, expansions can be done at 1 X 105 viable cells/mL by adding media to decrease the cell concentration.
This cell line forms tight clusters of viable cells accompanied by some single non-viable cells plus debris in suspension. With increased growth the clusters become larger. As the cell density nears 1 X 106 cells/mL, the single (non-clustered) ａnd non-viable cells increase along with the amount of debris.
At subculture, break up clusters by gentle pipetting.
Established cultures can be grown in T75 flasks.
Subcultivation Ratio: Add fresh medium every 2 to 3 days (depending on cell density)
Medium Renewal: Every 2 to 3 days 
Cryopreservation  Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase 
Culture Conditions  Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C

Isotype  IgM , IgM (surface)  
Population Doubling Time  about 20 hours  
Name of Depositor  NL Warner, LL Lanier
References  Boyd AW, et al. The regulation of growth ａnd differentiation of a murine B cell lymphoma. I. Lipopolysaccharide induced differentiation. J. Immunol. 126: 2461-2465, 1981. PubMed: 6785355

Lanier LL, Warner NL. Cell cycle related heterogeneity of Ia antigen expression on a murine B lymphoma cell line:analysis by flow cytometry. J. Immunol. 126: 626-631, 1981. PubMed: 6969756

Gutman GA, et al. Immunoglobulin production by murine B-lymphoma cells. Clin. Immunol. Immunopathol. 18: 230-244, 1981. PubMed: 6781803

mineral oil induced tumor in (BALB/c x NZB)F1 mice

To secrete IgM into the medium, these cells need to be stimulated with lipopolysaccharide (0.01 to 3 mcg/ml). 
 

References  Boyd AW, et al. The regulation of growth ａnd differentiation of a murine B cell lymphoma. I. Lipopolysaccharide induced differentiation. J. Immunol. 126: 2461-2465, 1981. PubMed: 6785355

Lanier LL, Warner NL. Cell cycle related heterogeneity of Ia antigen expression on a murine B lymphoma cell line:analysis by flow cytometry. J. Immunol. 126: 626-631, 1981. PubMed: 6969756

Gutman GA, et al. Immunoglobulin production by murine B-lymphoma cells. Clin. Immunol. Immunopathol. 18: 230-244, 1981. PubMed: 6781803

mineral oil induced tumor in (BALB/c x NZB)F1 mice