企业档案
易推广认证，请放心拨打
15000266580
上海复祥生物科技有限公司
会员类型：会员
已获得易推广信誉 等级评定
0成长值
(0 -40)基础信誉积累，可浏览访问
(41-90)良好信誉积累，可接洽商谈
(91+ )优质信誉积累，可持续信赖
易推广会员：13年
工商认证 【已认证】
最后认证时间：
注册号：**** 【已认证】
法人代表：【已认证】
企业类型：生产商【已认证】
注册资金：人民币万【已认证】
产品数：1133
参观次数：768372
手机商铺二维码
产品展示更多>>
当前位置： 易推广 > 生物试剂/抗体/细胞 > 生物制剂 > 其他 > 上海复祥生物科技有限公司 > 产品展示 > 细胞库 > 人正常细胞 > HBE135-E6E7人支气管上皮细胞
HBE135-E6E7人支气管上皮细胞
点击次数：277 发布时间：2018/4/3[更新时间:2024/11/14 14:28:50]
- 型 号：
- 价 格：面议
- 所在地点：中国大陆
- 产品完善度：
简单介绍： CRL-2959ATCC细胞|细胞系|细胞株|肿瘤细胞；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和*优培养条件！
| 产品简介
HBE135-E6E7人支气管上皮细胞细胞货期8-10个工作日
上海复祥生物提供 ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|,细胞库管理规范,提供的细胞株背景清楚,提供参考文献和*优培养条件,网站上有细胞照片,欢迎各位老师来电咨询!联系电话手机15000266580邮箱xiangfbio@163.com.
详情请登陆http://www.xiangbio.com/
上海复祥生物提供 ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|,细胞库管理规范,提供的细胞株背景清楚,提供参考文献和*优培养条件,网站上有细胞照片,欢迎各位老师来电咨询!联系电话手机15000266580邮箱xiangfbio@163.com.
详情请登陆http://www.xiangbio.com/
说明书：Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Remove ａnd discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask ａnd observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping, do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium ａnd aspirate cells by pipetting gently.
To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube ａnd spin at approximately 125 x g for 5 to 10 minutes.
Discard supernatant ａnd resuspend cells in fresh serum-free growth medium. Add appropriate aliquots of cell suspension to new culture vessels.
Place culture vessels in incubators at 37°C.
Subcultivation Ratio: 1:3 to 1:4
Medium Renewal: Every 2 to 3 days.
Note: For more information on enzymatic dissociation ａnd subculturing of cell lines consult Chapter 10 in Culture of Animal Cells: a Manual of Basic Technique by R. Ian Freshney, 3th edition, published by Alan R. Liss, N.Y., 1994.
培养条件：Keratinocyte-Serum Free medium with 5 ng/ml human recombinant EGF (do not filter) and 0.05 mg/ml bovine pituitary extract (Invitrogen, formerly GIBCO-BRL, Cat. No. 17005-042) and supplemented with 0.005 mg/ml insulin and 500 ng/ml hydrocortisone.
