© Chondrex, Inc. 2008 All Rights Reserved, 1.0, Page 1
Immunization Grade Chick Type II Collagen, Lyophilized
Catalog # 20011
For Research Use Only - Not Human or Therapeutic Use
DESCRIPTION:
Highly purified type II collagen (treated with 3M guanidine, DEAE-cellulose and Na2 HPO4 )
APPLICATION:
Can be used for various purposes, such as a standard for collagen analysis by SDS-gel, substrate
for MMP-1, MMP-8 and MMP-13, and as an antigen for immunizing animals for the induction of
collagen-induced arthritis (CIA) in certain species and strains of experimental animals.
QUANTITY:
10 mg
FORM:
Lyophilized powder
SOURCE:
Chick sternal cartilage
MOLECULAR WEIGHT: 300 Kd
PURITY:
>99% by SDS-PAGE, free of pepsin and proteoglycans
SOLUBILITY:
Soluble in acidic buffer (maximum4 mg/mL), but difficult to dissolve in neutral buffer. In order to dissolve
this product in a neutral buffer, use 0.01Mor 0.05Macetic acid to dissolve the collagen at 1-4 mg/mL and
then add this collagen solution to a desired neutral buffer such as 2-10X Tris-NaCl (the final concentration
of NaCl: 0.15-0.2M) or dialyze against 1X neutral buffer at 4°C.
Note 1: To avoid fibril formation under neutral conditions, keep solution on ice.
Note 2: The physicochemical property of type II collagen differs fromtype I collagen, and type II collagen
dissolved in a neutral buffer does not formstable gel with incubation at 37°C. In order to prepare
a type II collagen gel, additional components such as type I collagen or other substances might
be required.
STORAGE TEMPERATURE: 4°C in the dark
STABILITY:
2 years
REFERENCES:
Trelstad R. et al. Biochemistry 9: 4993-4998 (1970)
Miller EJ. Biochemistry 10: 1652-1659 (1971)
Trentham DE. et al. J. Exp. Med. 146: 857-868 (1977)
Wooley PH. et al. J. Immunology 135: 2443-2451 (1985)
原创作者:上海瓦兰生物科技有限公司