Grade VIII, lyophilized powder, ≥500 units/mg protein

别名: β-D-Galactoside galactohydrolase, Lactase

• CAS号 9031-11-2

•  Enzyme Commission (EC) Number 3.2.1.23  ( BRENDA | IUBMB )

•  EINECS 编号 232-864-1

•  MDL number MFCD00130623
  
  

  性质

  Related Categories	3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Application Index, Carbohydrate Hydrolysis, Carbohydrate ａnd PTM Analysis, Carbohydrate hydrolysis & PTM analysis, Carbohydrate-active Enzymes, Enzyme Class Index, Exoglycosidases, Reporter Gene Analysis ａnd Detection, 分子生物学, 生化试剂, 糖生物学, 纯化和检测, 翻译后修饰, 蛋白质组学, 酶、抑制剂和底物, 重组蛋白表达和分析 更多...
  Quality Level	PREMIUM
  type	Grade VIII
  form	lyophilized powder
  does not contain	BSA as extender
  composition	Protein, ≥60% biuret
  storage temp.	−20°C

  产品描述

  包装

  1000, 3000, 5000, 15000 units in serum bottle

  Unit Definition

  One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol ａnd D-galactose per min at pH 7.3 at 37 °C.

  Physical form

  Contains Tris buffer salts ａnd magnesium chloride

  Application

  β-Galactosidase is conjugated to an antibody which specifically recognizes a target molecule (enzyme immunoassay or EIA).^[1] β-Galactosidase is also used as a reporter enzyme to monitor the level of gene expression of a promoter.^[2] It may be used as a positive control protein with anti-β-galactosidase antibodies.

  Biochem/physiol Actions

  β-galactosidase cleaves lactose into its monosaccharide components, glucose ａnd galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

  General description

  β-Galactosidase is a tetramer consisting of four equal subunits of 135,000 each. It is a sulfhydryl containing enzyme, with 19 cysteine residues per subunit.^[3]

  Physical properties

  Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)