mol wt 150,000-300,000

• CAS号 25988-63-0

•  MDL number MFCD00237230

•  eCl@ss 32160406

•  PubChem Substance ID 24898197
  
  

  性质

  Related Categories	Adhesives, Amino Acids, Homopolymers, Polyamino Acids, Polylysine ａnd Polyornithine Attachment Factors, 生化试剂, 细胞生物学, 血液学和组织学 更多...
  form	solid
  mol wt	mol wt 150,000-300,000
  storage temp.	−20°C

  产品描述

  Components

  Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino ａnd either the carboxyl groups or N or O containing moieties.

  Analysis Note

  分子量基于粘度。同样通过 MALLS 分析。

  Application

  Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, ａnd chromosomal preparations. Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule.

  Caution

  无菌溶液可在2-8℃条件下稳定保存2年。脱水保存在-20°C条件下。

  包装

  1 g in poly bottle

  25, 100, 500 mg in poly bottle

  Preparation Note

  This product has a molecular weight of 150,000-300,000. To remove the HBr, dissolve this product in a neutral buffer ａnd dialyze to remove the salts. None of the poly-L-lysine products have been exposed to trifluoroacetic acid ａnd are dialyzed to remove any monomers, dimmers, or trimers, confirmed by thin layer chromatography. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, ａnd aseptically coat the surface with 1 mL per 25 cm² of solution. After 5 minutes, remove the solution through aspiration ａnd thoroughly rinse the surface. Let dry for two hours before introducing cells ａnd medium.

  Biochem/physiol Actions

  Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane ａnd the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.