for general use, Type I, 0.25-1.0 FALGPA units/mg solid, ≥125 CDU/mg solid

别名: 梭菌肽酶 A

• CAS号 9001-12-1

•  Enzyme Commission (EC) Number 3.4.24.3  ( BRENDA | IUBMB )

•  EINECS 编号 232-582-9

•  MDL number MFCD00130830
  
  

  性质

  Related Categories	3.4.x.x Peptidases, 3.x.x.x Hydrolases, Application Index, Cell Dissociation, Cell Dissociation ａnd Cell Lysis, Collagenases, Core Bioreagents, Core Bioreagents Enzymes, Crude Collagenase For General Use, Enzyme Class Index, Proteases, Proteases & Protein Sequencing, Proteolytic Enzymes, Proteolytic Enzymes ａnd Substrates, Research Essentials, 生化试剂, 酶、抑制剂和底物 更多...
  form	essentially salt-free, lyophilized powder
  mol wt	mol wt 68,000 to 125,000
  optimum pH	6.3-8.8
  solubility	TESCA buffer (50 mM TES, 0.36 mM Calcium chloride, pH 7.4): soluble 1-2 mg/mL at 37 °C
  storage temp.	−20°C

  产品描述

  Biochem/physiol Actions

  Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid ａnd 8-hydroxyquinoline.

  The collagenase product is a mixture of enzymes secreted by C. histolyticum, with different products differentiated by the relative ratios of the 10-18 components found in the secreted enzymes. The main components are two collagenases, clostripain, ａnd a neutral protease. The synergistic action of these enzymes degrade collagen ａnd other intracellular materialThe action of both collagenase enzymes ａnd the neutral protease is necessary for effective release of cells from tissue. Various types of collagen are the natural substrates for collagenase.

  Caution

  As supplied, this product is stable for one year at -20°C. There is no loss in FALGPA or protease activity in 30 days at 37°C, 50°C ａnd -20°C. Solutions of crude collagenase are stable if frozen quickly in aliquots (at 10 mg/mL) ａnd kept frozen at -20°C. Further freeze-thaw cycles will damage the solution. The product retains 100% activity over 7 hours when held on ice.

  包装

  1, 5 g in poly bottle

  100, 500 mg in poly bottle

  Preparation Note

  Equivalent to first 40% ammonium sulfate fraction of Mandl, I., et al., J. Clin. Invest., 32, 1323 (1953).

  Unit Definition

  One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.

  Application

  Collagenase from Clostridium histolyticum has extensive use in biological studies, where it is used to prepare isolated cell suspensions.^[3] This product is suitable for the disaggregation of human tumor, mouse kidney, human adult ａnd fetal brain, lung ａnd many other epithelia tissues. It has also been shown to be effective in liver ａnd kidney perfusion studies, digestion of pancreas, isolation of nonparenchymal rat liver cells ａnd hepatocyte preparation. Collagenase has also been used in the preparation of arterial tissue for the study of Advanced Glycosylation End Products. This enzyme has been tested for the release of heptatocytes at a concentration of approximately 1 mg/mL. Concentrations for digestion range from 0.1 to 5 mg/mL.

  General description

  Collagenase is a protease which cleaves the triple-helical protein called collagen. There are three types of tissue collagenases, ａnd these belong to the matrix metalloproteinases (MMP) family.^[1] Collagenase obtained from Clostridium histolyticum has a very strong activity, as it digests collagen from both ends, at temperatures as low as 4-10°C.^[2] Crude collagenase mixtures contain two major enzyme types namely, collagenase ａnd clostripain.^[3]